Mass spectrometry of triacylglycerols

Identification of very long chain fatty acids from sugar cane wax by atmospheric pressure chemical ionization liquid chromatography–mass spectroscopy

“…Still another vulnerable point is the entry into the MS and the mass spectrometer itself. Probably for these reasons the GC-MS has seldom been used for the analysis of triacylglycerols -see, e.g., Buchgraber et al, 2004;Laakso, 2002. As mentioned by Christie (2003), ''although useful separations of intact triacylglycerols by high-temperature GC can be achieved routinely, the technique can still be fraught with difficulties.…”

Identification of very long chain fatty acids from sugar cane wax by atmospheric pressure chemical ionization liquid chromatography–mass spectroscopy

“…Still another vulnerable point is the entry into the MS and the mass spectrometer itself. Probably for these reasons the GC-MS has seldom been used for the analysis of triacylglycerols -see, e.g., Buchgraber et al, 2004;Laakso, 2002. As mentioned by Christie (2003), ''although useful separations of intact triacylglycerols by high-temperature GC can be achieved routinely, the technique can still be fraught with difficulties.…”

Triacylglycerol accumulation and profiling in the model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum (Baccilariophyceae) during starvation

“…For GC-MS analysis, a high-temperature column (DB-5ht; 30 m×0.25 mm id×0.1 μm film thickness) was purchased from Agilent Technologies. By employing a high-temperature column, we eliminated the need for derivatization of each sample, which can result in artifacts due to overlapping peaks and cause inaccurate identification of the TAGs (Laakso 2002;Malavolta et al 2004).…”

Changes in the Composition of Triacylglycerols in the Fat Bodies of Bumblebee Males During Their Lifetime

“…The freshly emerged males were removed and kept according to age. Animals of the following age were studied: Bombus terrestris: shortly after eclosion, 1, 2, 3, 4, 5, 7, 10, 12, and 24 days after eclosion (5 individuals of each age); B. lucorum: shortly after eclosion, 1,2,3,4,5,6,7,8,9,10,15,20,25, and 30 days after eclosion (5 individuals of each age). The males were killed by freezing (-18°C) and kept frozen prior to dissection.…”

“…However, the intensity ratio depends also on the number and position of the double bonds in a particular FA. Therefore, if the TAG standards are not available, the positions of the FA on the glycerol backbone may be interpreted incorrectly [20]. The peak areas were calculated from reconstructed chromatograms for the [M ?…”

Identification of very long chain fatty acids from sugar cane wax by atmospheric pressure chemical ionization liquid chromatography–mass spectroscopy

“…Still another vulnerable point is the entry into the MS and the mass spectrometer itself. Probably for these reasons the GC-MS has seldom been used for the analysis of triacylglycerols -see, e.g., Buchgraber et al, 2004;Laakso, 2002. As mentioned by Christie (2003), ''although useful separations of intact triacylglycerols by high-temperature GC can be achieved routinely, the technique can still be fraught with difficulties.…”

Triacylglycerol accumulation and profiling in the model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum (Baccilariophyceae) during starvation

“…For GC-MS analysis, a high-temperature column (DB-5ht; 30 m×0.25 mm id×0.1 μm film thickness) was purchased from Agilent Technologies. By employing a high-temperature column, we eliminated the need for derivatization of each sample, which can result in artifacts due to overlapping peaks and cause inaccurate identification of the TAGs (Laakso 2002;Malavolta et al 2004).…”

Changes in the Composition of Triacylglycerols in the Fat Bodies of Bumblebee Males During Their Lifetime

“…The freshly emerged males were removed and kept according to age. Animals of the following age were studied: Bombus terrestris: shortly after eclosion, 1, 2, 3, 4, 5, 7, 10, 12, and 24 days after eclosion (5 individuals of each age); B. lucorum: shortly after eclosion, 1,2,3,4,5,6,7,8,9,10,15,20,25, and 30 days after eclosion (5 individuals of each age). The males were killed by freezing (-18°C) and kept frozen prior to dissection.…”

“…However, the intensity ratio depends also on the number and position of the double bonds in a particular FA. Therefore, if the TAG standards are not available, the positions of the FA on the glycerol backbone may be interpreted incorrectly [20]. The peak areas were calculated from reconstructed chromatograms for the [M ?…”

Identification of very long chain fatty acids from sugar cane wax by atmospheric pressure chemical ionization liquid chromatography–mass spectroscopy

“…Still another vulnerable point is the entry into the MS and the mass spectrometer itself. Probably for these reasons the GC-MS has seldom been used for the analysis of triacylglycerols -see, e.g., Buchgraber et al, 2004;Laakso, 2002. As mentioned by Christie (2003), ''although useful separations of intact triacylglycerols by high-temperature GC can be achieved routinely, the technique can still be fraught with difficulties.…”

Triacylglycerol accumulation and profiling in the model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum (Baccilariophyceae) during starvation

“…For GC-MS analysis, a high-temperature column (DB-5ht; 30 m×0.25 mm id×0.1 μm film thickness) was purchased from Agilent Technologies. By employing a high-temperature column, we eliminated the need for derivatization of each sample, which can result in artifacts due to overlapping peaks and cause inaccurate identification of the TAGs (Laakso 2002;Malavolta et al 2004).…”

Changes in the Composition of Triacylglycerols in the Fat Bodies of Bumblebee Males During Their Lifetime

“…The freshly emerged males were removed and kept according to age. Animals of the following age were studied: Bombus terrestris: shortly after eclosion, 1, 2, 3, 4, 5, 7, 10, 12, and 24 days after eclosion (5 individuals of each age); B. lucorum: shortly after eclosion, 1,2,3,4,5,6,7,8,9,10,15,20,25, and 30 days after eclosion (5 individuals of each age). The males were killed by freezing (-18°C) and kept frozen prior to dissection.…”

“…However, the intensity ratio depends also on the number and position of the double bonds in a particular FA. Therefore, if the TAG standards are not available, the positions of the FA on the glycerol backbone may be interpreted incorrectly [20]. The peak areas were calculated from reconstructed chromatograms for the [M ?…”