Mass spectral analysis of urine proteomic profiles of dairy cows suffering from clinical ketosis

Xu, Chuang; Sheng, Shi; Xia, Cheng; PengXian, Wang; Sun, Yuhang; Li, Changsheng

doi:10.1080/01652176.2015.1055352

Cited by 20 publications

(5 citation statements)

References 62 publications

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“…A comparative proteomic assessment of liver proteins and 5 enzymes (i.e., acetyl-coenzyme A acetyltransferase 2, 3-hydroxyacyl-CoA dehydrogenase type-2, elongation factor Tu, alpha-enolase and creatine kinase) revealed that they were differentially expressed in ketotic cows compared with healthy controls [54]. Moreover, urine concentrations of 11 proteins including amyloid precursor protein, apolipoprotein (Apo) C-III, cystatin C, fibrinogen, hepcidin, human neutrophil peptides, osteopontin, VGF (non-acronymic) protein, serum amyloid A (SAA), the C1 inhibitor (C1INH), and transthyretin were decreased in ketotic cows [55]. The same authors reported that serum fibroblast growth factor-21 might be a promising biomarker for detecting ketotic cows [56].…”

Section: Some Newly Identified Diagnostic Biomarkersmentioning

confidence: 99%

Ketosis an Old Story Under a New Approach

Zhang

Ametaj

2020

Dairy

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Ketosis, characterized by high concentrations of ketone bodies in the blood, urine, and milk, affects a considerable number of cows immediately after calving. Although much is known about ketosis, dairy cows continue to be affected in every herd world-wide. Cows affected by ketosis are treated with palliative treatments after the disease is diagnosed. This is a very expensive approach and costs the dairy industry extra expenses, contributing to lower profitability of dairy herds. In this review article, we summarize the mainstream view on ketosis, classification of ketosis into three types, current diagnostic approaches to ketosis, and the economic impact of ketosis on dairy farms. Additionally, we discuss the most recent applications of the new ‘omics’ science of metabolomics in studying the etiopathology of ketosis as well as its contribution in identification of novel screening or diagnostic biomarkers of ketosis.

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Section: Some Newly Identified Diagnostic Biomarkersmentioning

confidence: 99%

Ketosis an Old Story Under a New Approach

Zhang

Ametaj

2020

Dairy

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“…Meanwhile, a link between clinical ketosis in dairy cows and decreased milk production, rumen stagnation, constipation, decreased feed intake, as well as loss of body condition, and an association between hyperketosis and increased levels of oxidative stress has also been revealed [3]. Ketogenic cows have a lower milk yield, lower milk fat content, and higher concentrations of BHBA, NEFAs, interleukin (IL)-6, tumor necrosis factor (TNF), and lactic acid in serum compared with healthy cows [4,5,6], and the antioxidant capacity of ketone-causing bovine bodies is also greatly reduced [7]. High levels of NEFAs and BHBA are also associated with inflammatory responses during diseases such as cow mastitis and metritis.…”

Section: Introductionmentioning

confidence: 99%

Palmitic Acid and β-Hydroxybutyrate Induce Inflammatory Responses in Bovine Endometrial Cells by Activating Oxidative Stress-Mediated NF-κB Signaling

Cai

et al. 2019

Molecules

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Ketosis is a nutritional metabolic disease in dairy cows, and researches indicated that ketonic cows always accompany reproductive problems. When ketosis occurs, the levels of non-esterified fatty acids (NEFAs) and β-hydroxybutyrate (BHBA) in the blood increase significantly. Palmitic acid (PA) is a main component of saturated fatty acids composing NEFA. The aim of this study was to investigate whether high levels of PA and BHBA induce inflammatory responses and regulatory mechanisms in bovine endometrial cells (BEND). Using an enzyme-linked immunosorbent assay, quantitative real-time PCR, and western blotting, we evaluated oxidative stress, pro-inflammatory factors, and the nuclear factor (NF)-κB pathway in cultured BEND cells treated with different concentrations of PA, BHBA, pyrrolidinedithiocarbamate (PDTC, an NF-κB pathway inhibitor), and N-acetylcysteine (NAC, an antioxidant). The content of malondialdehyde was significantly higher, the content of glutathione was lower, and antioxidant activity—glutathione peroxidase, superoxide dismutase, catalase, and total antioxidant capacity—was lower in treated cells compared with control cells. PA- and BHBA-induced oxidative stress activated the NF-κB signaling pathway and upregulated the release of pro-inflammatory factors. Moreover, PA- and BHBA-induced activation of NF-κB-mediated inflammatory responses was inhibited by PDTC and NAC. High concentrations of PA and BHBA induce inflammatory responses in BEND cells by activating oxidative stress-mediated NF-κB signaling.

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“…One estrogen metabolite, not further specified, was found to discriminate between mild or strong and weak fatty liver in the same study. Beside liver and plasma, the difference of proteomic profiles in animals with ketosis were also explored in urine using SELDI-TOF techniques [91]. Samples were collected 7 -28 DIM from two groups of animals, one affected by clinical ketosis and the other from healthy cows, with a milk production of 9625 kg/year.…”

Section: Fatty Livermentioning

confidence: 99%

Proteomics and metabolomics characterizing the pathophysiology of adaptive reactions to the metabolic challenges during the transition from late pregnancy to early lactation in dairy cows

Ceciliani

Lecchi

Urh

et al. 2018

Journal of Proteomics

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For high-yielding dairy cows there are several "occupational diseases" that occur mainly during the metabolic challenges related to the transition from pregnancy to lactation. Such diseases and their sequelae form a major concern for dairy production, and often lead to early culling of animals. Beside the economical perspective, metabolic stress may severely influence animal welfare. There is a multitude of studies about the metabolic backgrounds of such so called production diseases like ketosis, fatty liver, or hypocalcaemia, although the investigations aiming to assess the complexity of the pathophysiological reactions are largely focused on gene expression, i.e. transcriptomics. For extending the knowledge towards the proteome and the metabolome, the respective technologies are of increasing importance and can provide an overall view of how dairy cows react to metabolic stress, which is needed for an in-depth understanding of the molecular mechanisms of the related diseases. We herein review the current findings from studies applying proteomics and metabolomics to transition-related diseases, including fatty liver, ketosis, endometritis, hypocalcaemia and laminitis. For each disease, a brief overview of the up to date knowledge about its pathogenesis is provided, followed by an insight into the most recent achievements on the proteome and metabolome of tissues and biological fluids, such as blood serum and urine, highlighting potential biomarkers. We believe that this review would help readers to be become more familiar with the recent progresses of molecular background of transition-related diseases thus encouraging research in this field.

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Mass spectral analysis of urine proteomic profiles of dairy cows suffering from clinical ketosis

Abstract: (2015) Mass spectral analysis of urine proteomic profiles of dairy cows suffering from clinical ketosis, Veterinary Quarterly, 35:3,[133][134][135][136][137][138][139][140][141]

Cited by 20 publications

References 62 publications

Ketosis an Old Story Under a New Approach

Ketosis an Old Story Under a New Approach

Palmitic Acid and β-Hydroxybutyrate Induce Inflammatory Responses in Bovine Endometrial Cells by Activating Oxidative Stress-Mediated NF-κB Signaling

Proteomics and metabolomics characterizing the pathophysiology of adaptive reactions to the metabolic challenges during the transition from late pregnancy to early lactation in dairy cows

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