Mass spectral analyses of microcystins from toxic cyanobacteria using on-line chromatographic and electrophoretic separations

Disposable Emitters for On-line Capillary Zone Electrophoresis/Nanoelectrospray Mass Spectrometry

“…The use of dynamic capillary derivatization with Polybrene for CZE/ES-MS has also been reported previously, 24,25 and is especially well suited to the butted-tip version of the nanospray interface, since the column and the tip can be coated relatively quickly and at the same time. The shortcomings of this method are the requirement for recoating between analyses, and the higher incidence of tip blockages from the multiple rinsing steps.…”

Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography

“…Biochemical methods such as enzyme-linked immunosorbent assay (ELISA) [18][19][20] and the protein phosphatase inhibition assay [5,21] are useful as screening methods due to their high sensitivity and high throughput, but they often have poor identification ability and the potential for false-positives. Reversedphase high-performance liquid chromatography (HPLC) with UV detection [20], diode array detection [22][23][24][25][26][27] or electrochemical detection [28], LC-mass spectrometry (LC-MS) [29][30][31], capillary electrophoresis (CE) [30], and CE-MS [30] have also been used for the identification and quantification of MCs. The LC method can be much more definitive than bioassays and biochemical methods 0003 since it can provide information for the identification of individual MCs.…”

Rapid discrimination between methicillin-sensitive and methicillin-resistant Staphylococcus aureus by intact cell mass spectrometry

“…Early work involved solvent extraction and chemical modification before analysis, but some methods use simple specimen preparation and direct analysis via appropriate ionisation methods [10]. Fast atom bombardment, electron impact and electrospray ionisation are extensively used for analysis of solvent extracts or volatile derivatives [11,12]. Prior separation of components of mixtures by chromatographic procedures, sometimes on-line by direct coupling to the mass spectrometer, has also been employed [13].…”