Mass Drug Administration Trial to Eliminate Lymphatic Filariasis in Papua New Guinea: Changes in Microfilaremia, Filarial Antigen, and Bm14 Antibody after Cessation

Tisch, Daniel J.; Bockarie, Moses J.; Dimber, Zachary; Kiniboro, Benson; Tarongka, Nandao; Hazlett, Fred E.; Kastens, Will; Alpers, Michael P.; Kazura, James W.

doi:10.4269/ajtmh.2008.78.289

Cited by 42 publications

(48 citation statements)

References 21 publications

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“…20 In our study, the prevalence of MF and intensity of antigenemia either remained static or significantly decreased ( P < 0.001), respectively, among children who received DEC. For children who received placebo, the intensity of antigenemia significantly increased ( P = 0.039) at the end of the study. As in other studies that showed decreased antibody responses to Bm14 by ELISA after DEC treatment, 20,[25][26][27][28] we showed decreases in antibody responses to Bm14 by MBA among children who received drugs containing DEC ( Figure 1A and B). Interestingly, antibody responses to Bm14 and Bm33 decreased among children who received drugs containing DEC, even among children who were MF-/ Og4C3-( Figures 1A and 2A ).…”

Section: Discussionsupporting

confidence: 60%

Multiplex Bead Assay for Serum Samples from Children in Haiti Enrolled in a Drug Study for the Treatment of Lymphatic Filariasis

Moss¹,

Priest²,

Boyd³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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A multiplex bead assay (MBA) was used to analyze serum samples collected longitudinally from children enrolled in a drug trial for treatment of filariasis in Leogane, Haiti. Recombinant antigens Bm14 and Bm33 from Brugia malayi, third polar tube protein (PTP3) from Encephalitozoon cuniculi, and merozoite surface protein-119 (MSP-119) from Plasmodium falciparum were coupled to carboxylated polystyrene microspheres. IgG responses to PTP3 and MSP-119 were not affected by albendazole (ALB), diethylcarbamazine (DEC), or combination of diethylcarbamazine and albendazole (DEC/ALB). However, IgG and IgG4 responses to Bm14 and Bm33 were significantly decreased (P < 0.001) by DEC and DEC/ALB treatment. Antibody responses to Bm14 and Bm33 decreased after DEC treatment (but not placebo) among children who were negative for microfilaremia and antigenemia at baseline, suggesting that these children harbored early stages of infection. The MBA is an excellent serologic technique for multiple antigens that offers substantial advantages over single-antigen based enzyme-linked immunosorbent assay in mass drug administration studies for monitoring changes in antibody levels.

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Section: Discussionsupporting

confidence: 60%

Multiplex Bead Assay for Serum Samples from Children in Haiti Enrolled in a Drug Study for the Treatment of Lymphatic Filariasis

Moss¹,

Priest²,

Boyd³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…Several other publications have evaluated the IgG4 antibody detection to the rBm14 antigen as a monitoring tool post-MDA; however, most of them have obtained the rBm14 antigen directly from Dr Gary Weil's laboratory in Washington DC before Cellabs Pty Ltd of Australia developed the new kit (CELISA) in 2006 [6,18,19]. A study conducted by Ramzy just after the 5th round of MDA in 2 Egyptian villages reported that the antibody prevalence rates can take several years to fall to low levels after MDA [6].…”

Section: Discussionmentioning

confidence: 99%

Surveillance of lymphatic filariasis 5 years after stopping mass drug administration in Menoufiya governorate, Egypt

Ma¹,

Hs²,

Ga³

et al. 2014

East Mediterr Health J

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The World Health Organization recommends that before lymphatic filariasis elimination in an area can be confirmed, an additional survey should be performed at least 5 years after stopping mass drug administration. The current study aimed to determine the status of lymphatic filariasis 5 years after cessation of the mass drug administration in 3 sentinel Egyptian villages in Menoufiya Governorate. The rapid immunochromatographic card test (ICT) and a new commercial antibody detection kit (CELISA®) were used. All 1321 primary-school children aged 6-7 years old were ICT negative but 27 children were antibody positive. All households surveyed in one village with the highest antibody prevalence were ICT negative, indicating an absence of lymphatic filariasis. The CELISA antibody kit needs more standardization and development to be useful under field conditions. We conclude that lymphatic filariasis is no longer a public health problem in these villages and other villages with similar epidemiological conditions.

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“…24,[29][30][31] All sequences were 100% identical with the W. bancrofti sequence in GenBank (accession no. AY297458).…”

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confidence: 97%

“…32 Furthermore, we sequenced the PCR product from five pooled third-stage larvae dissected from mosquitoes that were collected in the Dreikikir District (East Sepik Province, Papua New Guinea), where malaria and lymphatic filariasis are endemic. 24,[29][30][31] All sequences were 100% identical with the W. bancrofti sequence in GenBank (accession no. AY297458).…”

mentioning

confidence: 97%

“…28 We have been using the density of microfilariae in blood and an enzyme-linked immunosorbent assay (detection of Og4C3 antigen and anti-Bm14 IgG4) as measures of W. bancrofti infection in our ongoing lymphatic filariasis-related epidemiologic studies. [29][30][31] However, with decreasing prevalence of W. bancrofti infections, lower microfilaremia, and increasing importance of xenodiagnosis of infection in mosquitoes because of the anticipated success of filariasis elimination programs, DNA-based methods may be more efficient for performing the population-level diagnostic surveillance. Expanding our existing post-PCR LDR-FMA assay, we report the development of a multiplex assay that has the capability to simultaneously detect P. falciparum , P. vivax , P. malariae , and P. ovale , and W. bancrofti infections with high sensitivity and specificity in blood samples.…”

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confidence: 99%

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Molecular-Based Assay for Simultaneous Detection of Four Plasmodium spp. and Wuchereria bancrofti Infections

Mehlotra

Gray

Blood-Zikursh

et al. 2010

The American Society of Tropical Medicine and Hygiene

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† These authors contributed equally to this study. MULTIPLEX DETECTION OF MALARIA AND FILARIASIS PARASITESP. vivax , P. malariae , and P. ovale , 25 and W. bancrofti in a sequence-specific manner. The PCR reagents and conditions for Plasmodium spp. amplification have been described. 24,25 For the multiplex PCR, we evaluated the dNTP concentrations (dATP, dTTP, dGTP, and dCTP) from 200 μM to 800 μM to ensure nucleotide availability for the amplification of both Plasmodium spp. and W. bancrofti genomic regions, and added 0.12 μM of each of W. bancrofti UP (5¢-GATGGTGTATAATAGCAGCA-3¢) and W. bancrofti DN (5¢-GTCATTTATTTCTCCGTCGACTGTC-3¢) amplification primers to the PCR master mixture. The dNTP concentration that performed with consistently high efficiency was 400 μM. The PCR products were subjected to electrophoresis on agarose gels to visualize distinct Plasmodium spp. (491-500 basepairs) 25 and W. bancrofti (174 basepairs) amplicons. The PCR products were then subjected to LDR-FMA as described, 25 with minor modifications that included use of LDR primers: a W. bancrofti -specific primer (tacactttatcaaatcttacaatcTATATCTGCCCATAGAAATAACTA [sequence in lower case letters represents a 24-basepair oligonucleotide tag]) and a W. bancrofti common primer (Phos 5¢-CGGTGGATCTCTGGTTATCACTCTG-3¢Biotin). In the LDR-fluorescent microsphere hybridization solution containing Plasmodium species-specific fluorescent microspheres, 25 we added W. bancrofti -specific fluorescent micro sphere #3. Our W. bancrofti PCR and LDR primer sequences are based on the W. bancrofti sequence in GenBank (accession no. AY297458). 18To confirm the specificity of our W. bancrofti PCR primers, we amplified an approximately 170-basepair genomic DNA region from one microfilaria isolated from a person in Papua New Guinea and sequenced it. We also sequenced the PCR products from two microfilaria-positive persons from Papua New Guinea whose blood samples were collected as a part of ongoing studies.32 Furthermore, we sequenced the PCR product from five pooled third-stage larvae dissected from mosquitoes that were collected in the Dreikikir District (East Sepik Province, Papua New Guinea), where malaria and lymphatic filariasis are endemic. 24,[29][30][31] All sequences were 100% identical with the W. bancrofti sequence in GenBank (accession no. AY297458).The specificity of the assay was further demonstrated by using P. falciparum , P. vivax , P. malariae , and P. ovale , and W. bancrofti genomic DNA samples ( Figure 1 ). Using these genomic DNA samples individually in LDR-FMA reactions containing primers and microspheres for all five species, we found that the assay detected only the parasite species DNA present, and background signals for all other species DNAs were below a median fluorescence intensity of 500. We then performed multiplex PCR LDR-FMA diagnosis to detect Plasmodium spp. and W. bancrofti infections in the blood samples from 517 persons living in the Dreikikir District (East Sepik Province, Papua New Guinea).Using this assay, we found that 44...

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Mass Drug Administration Trial to Eliminate Lymphatic Filariasis in Papua New Guinea: Changes in Microfilaremia, Filarial Antigen, and Bm14 Antibody after Cessation

Cited by 42 publications

References 21 publications

Multiplex Bead Assay for Serum Samples from Children in Haiti Enrolled in a Drug Study for the Treatment of Lymphatic Filariasis

Multiplex Bead Assay for Serum Samples from Children in Haiti Enrolled in a Drug Study for the Treatment of Lymphatic Filariasis

Surveillance of lymphatic filariasis 5 years after stopping mass drug administration in Menoufiya governorate, Egypt

Molecular-Based Assay for Simultaneous Detection of Four Plasmodium spp. and Wuchereria bancrofti Infections

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