Maspin is expressed in the nuclei of breast myoepithelial cells

Reis‐Filho, Jorge S.; Milanezi, Fernanda; Schmitt, Fernando

doi:10.1002/path.1104

Cited by 21 publications

(22 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies describe maspin as a soluble cytoplasmic protein that associates with secretory vesicles and is also present on the cell surface [21,27]. We [23,24,25] and others [12,17,20] have demonstrated that maspin is also expressed in the nuclei of myoepihelial cells, myoepithelial cell tumors, and in some breast carcinomas [12,20,23,24,25]. However, in the present study we report for the first time, maspin expression in the nuclei of scattered neoplastic cells in SCCs and BCCs.…”

Section: Discussionsupporting

confidence: 38%

“…Maspin is one of the serpins that have received great attention by basic researchers and pathologists during the past few years [9,5,6,11,12,14,15,16,17,18,20,21,22,23,24,25,27,28,31,35,36,37]. Since its characterization several biological properties have been attributed to maspin, including tumor suppressor and antiangiogenic activities [9,14,15,22,27,28,36,37].…”

Section: Discussionmentioning

confidence: 99%

“…Since its characterization several biological properties have been attributed to maspin, including tumor suppressor and antiangiogenic activities [9,14,15,22,27,28,36,37]. It is also considered as a suppressor of invasion, motility, proliferation, and metastatic potential of neoplastic cells [9,5,6,11,12,14,15,16,17,18,20,21,22,23,24,25,27,28,31,35,36,37]. Moreover, there are controversial data on the role of maspin as a downstream factor of p53 pathway [9,14,18,33,37,38].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it has been reported that maspin is also expressed in the nuclei of myoepithelial [23,25], pancreatic [17], and breast secretory cells [20]. These findings prompted us to evaluate the hypothesis that maspin expression is also associated with specific differentiations in some cell types.…”

Section: Introductionmentioning

confidence: 98%

“…Recently our group [23,25] and others [12,17,20] demonstrated that maspin is consistently expressed in normal and hyperplastic myoepithelial cells and in myoepithelial cell tumors. Moreover, we also observed that peculiarly aggressive types of breast neoplasms do express maspin [24] despite their very aggressive behavior.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Maspin expression in normal skin and usual cutaneous carcinomas

et al. 2002

View full text Add to dashboard Cite

Maspin is a serine protease inhibitor whose gene is located on 3q27. Several lines of evidence point towards its putative role as tumor suppressor gene and angiogenesis inhibitor; however, there are compelling data showing that maspin is also expressed in the nuclear compartment and might be associated with the differentiation of specific cell lineages. No systematic study of maspin expression in normal skin and usual skin carcinomas have been published so far. We semiquantitatively analyzed the distribution and immunoreactivity pattern of maspin in 14 squamous cell carcinomas (SCCs) and 16 basal cell carcinomas (BCCs) and in the adjacent normal epidermis of all cases. We also examined the correlation of maspin expression with histological type, grade, vascular invasion, perineural infiltration, and mitotic counting. Cytoplasmic expression of maspin was observed in suprabasal, prickle, and granular cell layers of normal epidermis; cells of the germinative hair matrix, Henle's and Huxley's layers, and cuticle of hair follicles; mature sebaceous cells and sweat gland's secretory cells. Nuclear expression was detected in some basal/myoepithelial cells of the sweat glands and scattered mature sebaceous cells. All SCCs but one grade IV SCC showed maspin expression, and it was correlated with the differentiation of these neoplasms. BCCs presented variable maspin expression, while metatypical carcinomas showed moderate to intense maspin expression, nodular BCCs variable contents of maspin and displayed a peculiar distribution, confined to the center of the neoplastic nodules. Two BCCs and one SCC showed maspin nuclear expression. No correlation with other clinical pathological features was observed. Our findings do not support the role of maspin as a tumor suppressor gene and suggest that this serpin is probably associated with specific lines of differentiation.

show abstract

Section: Discussionsupporting

confidence: 38%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Maspin expression in normal skin and usual cutaneous carcinomas

et al. 2002

View full text Add to dashboard Cite

show abstract

Serpin B5 is a CEA‐interacting biomarker for colorectal cancer

Baek

Yeo

Chang

et al. 2013

Intl Journal of Cancer

View full text Add to dashboard Cite

Serpin B5 is a candidate tumour suppressor, but its oncogenic activity has also been reported. Its function may be affected by protein interactions. The aim of this study was to assess the relationship between serpin B5 and carcinoembryonic antigen (CEA) expression in colorectal cancer (CRC). We also analysed the clinicopathological significance of serpin B5 expression in patients with CRC. Downregulation of serpin B5 was identified in a CEA-suppressed LoVo cell line using two-dimensional gel electrophoresis (2-DE) and matrix-associated laser desorption ionisation-mass spectrometry (MALDI-MS). The specific interaction and co-localisation of serpin B5 with CEA were confirmed by co-immunoprecipitation and confocal microscopy. Western blot analysis and ELISAs revealed significant positive correlations between levels of serpin B5 and CEA in human colon cancer cell lines and in the blood of patients with CRC. Tissue expression of serpin B5 in 377 patients with CRC was significantly associated with serum CEA, histological grade, stage, lymph node metastasis, lymphatic and perineural invasion, and infiltrative border. Strong expression of serpin B5 was also associated with a reduced DFS (p 5 0.001) and OS (p 5 0.017). Together, these findings describe a relationship between serpin B5 and CEA expression in CRC. Strong expression of serpin B5 was associated with a worse prognosis in patients with CRC and its expression may correlate with CEA levels in CRC.

show abstract

The Opportunity of Precision Medicine for Breast Cancer With Context‐Sensitive Tumor Suppressor Maspin

Bernardo

Dzinic

Matta

et al. 2017

J of Cellular Biochemistry

View full text Add to dashboard Cite

To improve the precision of molecular diagnosis and to develop and guide targeted therapies of breast cancer, it is essential to determine mechanisms that underlie the specific tumor phenotypes. To this end, the application of a snapshot of gene expression profile for breast cancer diagnosis and prognosis is fundamentally challenged since the tissue-based data are derived from heterogonous cell types and are not likely to reflect the dynamics of context-dependent tumor progression and drug sensitivity. The intricate network of epithelial differentiation program can be concertedly controlled by tumor suppressor maspin, a homologue of clade B serine protease inhibitors (serpin), through its multifaceted molecular interactions in multiple subcellular localizations. Unlike most other serpins that are expressed in multiple and cell types, maspin is epithelial specific and has distinct roles in luminal and myoepithelial cells. Endogenously expressed maspin has been found in the nucleus and cytoplasm, and detected on the surface of cell membrane. It is also secreted free and as an exosomal cargo protein. Research in the field has led to the identification of the maspin targets and maspin-associated molecules, as well as the structural determinants of its suppressive functions. The current review discusses the possibility for maspin to serve as a cell type-specific and context-sensitive marker to improve the precision of breast cancer diagnosis and prognosis. These advancements further suggest a new window of opportunity for designing novel maspin-based chemotherapeutic agents with improved anti-cancer potency.

show abstract

Maspin is expressed in the nuclei of breast myoepithelial cells

Cited by 21 publications

References 5 publications

Maspin expression in normal skin and usual cutaneous carcinomas

Maspin expression in normal skin and usual cutaneous carcinomas

Serpin B5 is a CEA‐interacting biomarker for colorectal cancer

The Opportunity of Precision Medicine for Breast Cancer With Context‐Sensitive Tumor Suppressor Maspin

Contact Info

Product

Resources

About