Markers of adventitious root formation

Klerk, GJ De

doi:10.1051/agro:19961003

Cited by 27 publications

(18 citation statements)

References 26 publications

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“…Plant peroxidases are known to be involved in auxin metabolism as well as lignification processes in the cell wall in the presence of phenol, and the present results showed that peroxidase activity increased later than IAA-oxidase activity which indicates that peroxidase activity is more involved in cell wall synthesis at the later phase and obligatory step in root formation (Sato et al 1993). Auxin-induced changes in peroxidase and IAA-oxidase during the rooting process has also been reported (Fett-Neto et al 1992;DeKlerk 1996;Liu et al 1996;Mato et al 1988). Polyphenoloxidase activity increased both in IBA-treated and control-treated cuttings during induction and initiation phase but declined slowly during expression phase (Figure 4).…”

Section: Biochemical Changes During Rootingsupporting

confidence: 75%

Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes

Rout

2006

Plant Growth Regul

119

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An attempt was made to induce rooting from single node cuttings of Camellia sinensis var. TV-20 under controlled conditions and study its biochemical changes during rooting. The nodal cuttings were pretreated with different concentrations of IAA, NAA and IBA and kept in a growth chamber (25 ±2°C, 16 h photoperiod (55 lmol m À2 s À1 ) with cool, white fluorescent lamps and 65% relative humidity) for 12 h. Among the three auxins used for pretreatment, IBA showed more positive response on rooting as compared to IAA and NAA within 2 weeks of transfer to potting medium. Among four concentrations of IBA tested, 75 ppm gave maximum percentage of rooting, number of roots and root length. Therefore, IBA was used further in experiments for biochemical investigation. The adventitious rooting was obtained in three distinct phases i.e. induction (0-12 days), initiation (12-14 days) and expression (14-18 days). IAA-oxidase activity of IBAtreated cuttings increased slightly as compared to control. The activity was found to decrease during induction and initiation phases and increase during expression phase. The peroxidase activity in IBA-treated cuttings increased up to initiation phase and declined at the expression phase. Polyphenoloxidase activity increased both in IBA-treated and control cuttings during induction and initiation phase but declined slowly during expression phase. Total phenolic content was higher in IBA-treated cuttings, particularly in initiation and expression phases and it also correlated with peroxidase activity. Phenolics might be playing key role for induction of adventitious rooting, and phenolic compounds can be used as rooting enhancer in tea plant.Abbreviations: IAA -indole-3-acetic acid; IBA -indole-3-butyric acid; NAA -1-naphthaleneacetic acid

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Section: Biochemical Changes During Rootingsupporting

confidence: 75%

Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes

Rout

2006

Plant Growth Regul

119

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“…At day 10, when root primordia were well formed, starch levels in the pith and the periphery of root primordia appeared to decrease further, although some starch grains were still associated with the primordia ( Figure 5G). These starch grains are expected to be consumed as energy and carbon skeletons for the further development of adventitious roots (De Klerk 1996;Haissig 1974;Haissig 1986;Já sik and De Klerk 1997). Very few starch grains were observed in the section from the hypocotyl cuttings of nonrooting controls ( Figure 5H).…”

Section: Changes In Starchmentioning

confidence: 99%

“…A better understanding of the internal physiology of the rooting process and knowledge of potential biochemical markers of this process in radiata pine would be advantageous for further development of clonal forestry based on radiata pine shoot cuttings. Research on other species suggests that biochemical changes, such as protein, enzyme, and carbohydrate changes, are involved in the rooting process (Bhattacharya 1988;De Klerk 1996;Gonzá lez and others 1991;Haissig 1986;Hand 1994;Jarvis 1986). To date reports on radiata pine in this area, however, are lacking.…”

Section: Introductionmentioning

confidence: 99%

Starch Accumulation Is Associated with Adventitious Root Formation in Hypocotyl Cuttings of Pinus radiata

Leung

2000

J Plant Growth Regul

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This study of the internal physiology of adventitious root formation in Pinus radiata was performed without the potential complications from microbial contamination and nutritional stress. Hypocotyl cuttings of radiata pine were cultured in half strength MS nutrient medium supplemented with IBA (indole-3-butyric acid), IBA + kinetin, kinetin, or without phytohormones (control). Averages of 8.35 and 0.08 roots per cutting were formed in IBA and in growth regulator-free treatments, respectively. No roots were formed in IBA + kinetin, kinetin, or sucrose-free treatments at day 30 after excision of hypocotyls. Changes in fresh weight, sugar, and starch content were measured at established developmental stages associated with adventitious root formation. Sucrose-supplemented medium was required for higher levels of sugar, starch, and root formation in rooting region of IBA-treated hypocotyls. Starch accumulation, in particular, seems to have potential as a biochemical marker before root primordium emergence in light of the following observations in the IBA treatment. Starch began to build up preferentially in cells involved in or in close proximity to potential sites of new root primordium formation (that is, the cells on the inside of the cortex and the pith) before any visible organized root primordia and then began to disappear during root primordium formation. The substantial starch accumulation associated with IBA treatment was not observed in the IBA + kinetin, kinetin alone, growth regulator-free, sucrose-free treatments (nonrooting treatments) or in the nonrooting region of hypocotyls treated with IBA.

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“…Micropropagation also provides the possibility of controlling growth by modifying the biochemical and physical environment, which in turn could influence secondary metabolism, i.e., the adjustment of culture conditions could lead to optimised alkaloid production. Rooting differentiation and growth are often critical and difficult to perform during in vitro propagation of woody species (De Klerk 1996;. To overcome this difficulty, a rooting culture technique using successive media was successfully employed (Martínez ).…”

Section: Introductionmentioning

confidence: 99%

Polyamines and inhibitors used in successive culture media forin vitrorooting inBerberis buxifolia

Arena¹,

Pastur

Benavides

et al. 2005

New Zealand Journal of Botany

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Although Berberis buxifolia has been reported to propagate in vitro, rooting is difficult to achieve during subcultures, with reduced quantity and quality of roots, as is the case with many other woody species. Several cofactors of rhizogenesis have been proposed, and the role of polyamines in rooting has recently acquired relevance. Polyamines can stimulate or inhibit microshoot rooting depending on the type and concentration of polyamine and rooting phase, while polyamine inhibitors can improve rooting in some cases. A study of the in vitro rooting of B. buxifolia using polyamines and a mix of polyamine inhibitors in a two-step culture medium is described here, in which a new successive rooting medium was successfully implemented. While polyamine inhibitors sometimes improved rooting, nutrient medium containing a low polyamine concentration (i.e., 1 µM) enhanced rooting compared with the control medium. The best microshoot rooting response resulted from the addition of 1 µM spermidine during the expression phase in the absence of inhibitors in both rooting phases. Overall, incorporating polyamine in the successive media enhances the quality and quantity of roots in B. buxifolia, thus confirming their role during the induction and expression of the radicle primordia differentiation.

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Markers of adventitious root formation

Cited by 27 publications

References 26 publications

Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes

Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes

Starch Accumulation Is Associated with Adventitious Root Formation in Hypocotyl Cuttings of Pinus radiata

Polyamines and inhibitors used in successive culture media forin vitrorooting inBerberis buxifolia

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