Markers for the population genetics studies of Triatoma sordida (Hemiptera: Reduviidae)

Belisário, Carlota Josefovicz; Pessoa, Grasielle Caldas D’Ávila; Santos, Paula Fernandes dos; Dias, Letícia Sena; Rosa, Aline Cristine Luiz; Diotaiuti, Liléia

doi:10.1186/s13071-015-0879-1

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…fluviatilis in this study was similar to that of other Aedes species [ 60 , 61 ], indicating that the results represent the genuine allelic richness and are not the result of limitations of the loci. The private allelic richness found in the populations was moderate, suggesting some degree of isolation among the populations, although this may be related to the fact that the microsatellite primers used were originally designed for other species of Aedes , which could also explain the presence of null alleles [ 31 , 62 , 63 ].…”

Section: Discussionmentioning

confidence: 99%

Population Genetic Structure of Aedes fluviatilis (Diptera: Culicidae)

et al. 2016

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Although Aedes fluviatilis is an anthropophilic mosquito found abundantly in urban environments, its biology, epidemiological potential and genetic characteristics are poorly understood. Climate change and urbanization processes that result in environmental modifications benefit certain anthropophilic mosquito species such as Ae. fluviatilis, greatly increasing their abundance in urban areas. To gain a better understanding of whether urbanization processes modulate the genetic structure of this species in the city of São Paulo, we used eight microsatellite loci to genetically characterize Ae. fluviatilis populations collected in nine urban parks in the city of São Paulo. Our results show that there is high gene flow among the populations of this species, heterozygosity deficiency and low genetic structure and that the species may have undergone a recent population expansion. There are two main hypotheses to explain these findings: (i) Ae. fluviatilis populations have undergone a population expansion as a result of urbanization; and (ii) as urbanization of the city of São Paulo occurred recently and was quite intense, the structuring of these populations cannot be observed yet, apart from in the populations of Ibirapuera and Piqueri parks, where the first signs of structuring have appeared. We believe that the expansion found in Ae. fluviatilis populations is probably correlated with the unplanned urbanization of the city of São Paulo, which transformed green areas into urbanized areas, as well as the increasing population density in the city.

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Section: Discussionmentioning

confidence: 99%

Population Genetic Structure of Aedes fluviatilis (Diptera: Culicidae)

et al. 2016

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“…Transferability of microsatellite loci between close taxa have been successfully performed before [ 12 , 13 ], reducing primer development cost encouraging studies of population genetic structure in species with no specific developed microsatellite loci. Our objective was to survey the transferability to Ae.…”

Section: Resultsmentioning

confidence: 99%

“…Although caution is recommended regarding the use of microsatellite loci that may have null alleles, as they are less informative and may be more ambiguous, they still could be included in the analysis if their bias do not influence the results significantly. [ 13 , 23 , 25 ]. Also, cross-species microsatellite loci transfer might result in an ascertainment bias, which means that rates of variability may be consistently lower in the new species than in the species for which the markers were developed.…”

Section: Resultsmentioning

confidence: 99%

Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies

2015

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BackgroundAedes fluviatilis is a neotropical mosquito species thought to be a potential vector of Yellow Fever viruses and can be infected with Plasmodium gallinaceum in laboratory. A better understanding of its genetic structure is very important to understand its epidemiologic potential and how it is responding to urbanization. The objective of this study was to survey the transferability of microsatellites loci developed for other Aedes to Ae. fluviatilis.FindingsWe tested in Ae. fluviatilis 40 pairs of primers known to flank microsatellite regions in Aedes aegypti, Aedes albopictus and Aedes caspius, and found eight loci that amplified consistently. The number of alleles per locus ranged from 2 to 15, and the expected heterozygosity ranged from 0.09 to 0.85.ConclusionsWe found that several microsatellite primers successfully transferred to Ae. fluviatilis. This finding opens avenues for cost-effective optimization of high-resolution population genetic tools.

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“…pipens; Wilke et al 88 com Cx. quinquefasciatus, Angêlla et al 89 com Anopheles darlingi e Belisário et al 90 com Triatoma sordida.…”

Section: Morfometria Geométrica Alarunclassified

Análise da estrutura populacional de mosquitos <i>Aedes aegypti</i>(Diptera: Culicidae) em diferentes estratos urbanos na cidade de São Paulo, utilizando morfometria geométrica alar e marcadores microssatélites

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Markers for the population genetics studies of Triatoma sordida (Hemiptera: Reduviidae)

Cited by 6 publications

References 16 publications

Population Genetic Structure of Aedes fluviatilis (Diptera: Culicidae)

Population Genetic Structure of Aedes fluviatilis (Diptera: Culicidae)

Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies

Análise da estrutura populacional de mosquitos <i>Aedes aegypti</i>(Diptera: Culicidae) em diferentes estratos urbanos na cidade de São Paulo, utilizando morfometria geométrica alar e marcadores microssatélites

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