Marker assisted selection for resistance to Striga gesnerioides in Cowpea (Vigna unguiculata L. Walp)

Larweh, Victoria; Akromah, R.; Amoah, Stephen; Asibuo, James Yaw; Prempeh, Ruth; Kusi, Francis

doi:10.31248/jbbd2017.047

Cited by 3 publications

(2 citation statements)

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“…The PCR was carried out in a reaction volume of 25 μL, containing 12.5 μL of commercial Mix PCR (OneTaq Quick‐Load 2X Master Mix with Standard Buffer, Biolab Inc), 3 μL of genomic DNA (100 ng/μl), 0.5 μL of each primer (10 μM) (Table 1) and 8.5 μL of distilled autoclaved water. The following cycles were performed: ‘initial denaturation (95°C, 1 min); 35 denaturation cycles at 94°C, for 1 min; annealing (55 or 60°C, 1 min); 1 cycle of 72°C for 1 min for elongation and one 72°C cycle for 10 min for final elongation’ (Asare et al, 2013; Larweh et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Identification of sources of resistance to Striga gesnerioides Willd. Vatke among cowpea (Vigna unguiculata [L.] Walp) germplasm from Togo

Kondi,

Mawuli,

Timko

et al. 2023

Plant Breeding

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Striga gesnerioides is one of the major biotic constraints to cowpea (Vigna unguiculata [L.] Walp) production throughout West Africa. The best way to control and limit the spread of this weed remains genetic control, but recent works have shown that all cowpea varieties commonly grown in Togo are susceptible to S. gesnerioides. This study aims to investigate the resistance response of cowpea germplasm from Togo to S. gesnerioides. A field test was performed under natural infestation followed by a pots test under artificial infestation. Genotyping was carried out with three molecular markers SSR1, 61RM2 and C42‐2B, known for their linkage to S. gesnerioides resistance genes. The results have revealed three cowpea accessions, TG20_66, TG20_82 and TG20_108, phenotypically resistant to S. gesnerioides. All three resistant accessions have shown the RSG3‐301 resistance gene presence. Potential resistance gene sources to S. gesnerioides exist among cowpea accessions from Togo.

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Section: Methodsmentioning

confidence: 99%

Identification of sources of resistance to Striga gesnerioides Willd. Vatke among cowpea (Vigna unguiculata [L.] Walp) germplasm from Togo

Kondi,

Mawuli,

Timko

et al. 2023

Plant Breeding

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“…e study in [30] produced a PIC valve ranging from 0.08 to 0.60 with a mean valve of 0.34 from a total of 4.47. Also, Larweh et al [32] noted that polymorphism information content (PIC) values ranged from 0.32 to 0.36 with a mean of 0.34 in cowpea breeding lines. Ali et al [33] reported that a mean PIC value ≥ 0.5 is highly informative, 0.25∼0.50 reasonably informative, and <0.25 slightly informative, and loci (marker) with many alleles and a PIC value near 1 are most desirable.…”

Section: Genotypementioning

confidence: 99%

Assessment of Striga gesnerioides (Willd.) Resistance and Genetic Characterization of Forty-Six Cowpea (Vigna unguiculata (L.) Walp.) Genotypes in Ghana

Haruna

Asare

Kusi³

2020

International Journal of Agronomy

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The parasitic weed, Striga gesnerioides, imposes physiological stress on cowpea (Vigna unguiculata (L.) Walp.) resulting in significant yield loss in the regions of northern Ghana. This warranted identification of resistant cowpeas for sustainable production. The current work aim was to identify Striga-resistant cowpea genotypes and assess their genetic relatedness. Forty-six (46) cowpea genotypes were screened in pots for their reaction to Striga samples obtained from the upper east, upper west, and northern regions of Ghana and validated with C42-2B and 61R-M2 markers involving DNA amplification by PCR assay. Sixteen polymorphic SSR primer pairs were used to assess genetic relatedness among 46 cowpea genotypes. Data were analyzed with PowerMarker V. 3.25 and a dendrogram was generated with MEGA 4. On the whole, 65.2% of the cowpea genotypes had stable resistance to S. gesnerioides from the regions of northern Ghana and 34.8% were susceptible. The C42-2B marker resolved as a single DNA band of 280 bp with segregation efficiency of 80% and 61R-M2 marker as double DNA bands of 320 bp and 380 bp with segregation efficiency of 60% associated with Striga resistance. Sixteen (16) polymorphic SSR primers distinguished all 46 cowpea genotypes into three clusters. Gene diversity ranged from 0.04 to 0.49 with an average of 0.29. The average allele frequency is 0.78, with a mean genetic diversity of 0.29. Polymorphism information content (PIC) varied from 0.08 to 1.00 with an average of 0.55. Therefore, cowpeas with Striga resistance and other desirable traits can be evaluated and released as varieties for farmers to cultivate.

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Farmers and Agricultural Extension Officers Perception ofStriga gesnerioides(Willd.) Vatke Parasitism on Cowpea in the Upper East Region of Ghana

Haruna

Asare

Asare‐Bediako

et al. 2018

Advances in Agriculture

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Cowpea [Vigna unguiculata(L.) Walp] is an important food security crop in Sub-Saharan Africa. However, cowpea production in some parts of this region is constrained byStriga gesnerioides(Willd.) Vatke infestation resulting in yield losses. This study assessed the farmers and agricultural extension officers (AEOs) perception ofS. gesnerioidesparasitism on cowpea in the Upper East Region of Ghana. In all, 200 respondents—179 cowpea farmers and 21 AEOs—in five cowpea growing districts were randomly sampled to answer closed- and opened-ended questions coupled with visit to cowpea farms. Majority of the farmer respondents were males between the ages of 21 and 60 with very little or no formal education. Almost all of the respondents could identify the parasitic weed,S. gesnerioides,but they lacked adequate knowledge of the parasitic effects on the host, cowpea plant.S. gesnerioidesinfestation was widespread and devastating in almost all the fields surveyed. Its effects on the host plants (cowpea) included stunted growth, leaf senescence, chlorosis, death of the crop, and reduced yield. Different management strategies employed by the farmers to contain the spread of the parasite include weeding (90%), application of manure (5.5%), and crop rotation and fallowing (4.0%). Both farmers and extension officers had very little knowledge on existence ofS. gesnerioidesresistant cowpea genotypes for cultivation. Farmers expressed the desire to cultivateStriga-resistant cowpea varieties when made accessible, since it is the most reliable and environmentally friendly method of controlling the parasitic weed.

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Marker assisted selection for resistance to Striga gesnerioides in Cowpea (Vigna unguiculata L. Walp)

Cited by 3 publications

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Identification of sources of resistance to Striga gesnerioides Willd. Vatke among cowpea (Vigna unguiculata [L.] Walp) germplasm from Togo

Identification of sources of resistance to Striga gesnerioides Willd. Vatke among cowpea (Vigna unguiculata [L.] Walp) germplasm from Togo

Assessment of Striga gesnerioides (Willd.) Resistance and Genetic Characterization of Forty-Six Cowpea (Vigna unguiculata (L.) Walp.) Genotypes in Ghana

Farmers and Agricultural Extension Officers Perception ofStriga gesnerioides(Willd.) Vatke Parasitism on Cowpea in the Upper East Region of Ghana

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