Marker-assisted selection

Arús, Pere; Moreno-González, J.

doi:10.1007/978-94-011-1524-7_20

Cited by 44 publications

(21 citation statements)

References 82 publications

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“…Until recently, the genetic markers used to determine genetic diversity and to identify cultivars have been isozymes, other proteins, or those affecting morphological characters. Unfortu-nately, these markers are either limited in number and/or cause such large effects on phenotype that they are not suitable for breeding programs (ARUS and MORENO-GONZALES 1993;TANKSLEY et al 1989). On the other hand, DNA markers do not have such limitations.…”

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Genetic Characterization of two Eragrostis Species using AFLP and Morphological Traits

et al. 2004

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Ayele, M., Tefera, H., Assefa, K. and Nguyen, H. T. 1999. Genetic characterization of two Eragrostis species using AFLP and morphological traits.-HHereditu.r 130: 33-40. Lund, Sweden. lSSN 0018-0661. Received August 31, 1998. Accepted January 11, 1999 Tef [Eragrosris tef (Zucc.) Trotter] is the most important cereal crop in Ethiopia. An experiment was conducted to investigate genetic diversity among four cultivars of tef and 14 accessions of Eragrostis pifosa using radiobabelled and silver stained amplified fragment length polymorphism. Morphological traits were also evaluated. A total of 897 markers were obtained out of which 395 were polymorphic using 1 1 primer combinations. Cluster analysis revealed accessions of E. pilosa which are distantly related and others closely related to tef. Our previous experience also indicates that E. pilosa is crossable with tef. Those accessions distantly related to tef could be used in a crossing program to generate a population for selection and/or genetic mapping. Such genetic mapping populations will form an important entry point towards the molecular genetic dissection of the plant genus, Erqrostis, especially in the context of comparative mapping. Knowledge gained from such study, apart from tef improvement, will also be useful for many forage and turf grass species where little molecular genetic information is available. Nine cultivars or accessions had one or more unique fragments using one or more AFLP primers indicating the potential of the technology in fingerprinting tef in a breeding or seed multiplication program. The results also showed that clusters obtained using silver staining and y"P-ATP labeling were similar, suggesting that silver staining could be used as an alternative to radiolabeling at least in genetic diversity analysis. Significant genetic variation was obtained for morphological traits. Of particular interest to tef breeding was short plant stature in E. pi1o.w which could be transferred to tef to minimize the problem of lodging. Diversity revealed at the morphological trait level was not commensurate with that observed for AFLP. This was due to the small number of available morphological traits and their interaction with the environment. Henrj,

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Genetic Characterization of two Eragrostis Species using AFLP and Morphological Traits

et al. 2004

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“…The utility of MAS in these instances depends upon the ability to identify these blocks of genes (the effective factors of Mather, 1973) or as they are now generally known 'Quantitative Trait Loci' (QTL's). The development of the extensive range of polymorphic markers previous outlined provides a most powerful tool for locating QTLs by means of genetic mapping procedures (Soller & Beckmann, 1983;Stuber et al, 1987;Paterson et al, 1988;Lander & Botstein, 1989;Arus & Moreno-Gonzalez, 1993).…”

Section: Linkage Of the Marker To The Selected Trait Locusmentioning

confidence: 99%

Genetic markers and the selection of quantitative traits in forage grasses

et al. 1994

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“…Progeny of specific progenitors can be selected on the basis of molecular markers as long as these are associated with breeding values of the traits under consideration. This is one form of MAS (Dekkers and Dentine 1991;Arus and Moreno-Gonzalez 1993). Another form of MAS is based on the molecular selection index (MSI) proposed by Lande and Thompson (1990).…”

Section: Arker-assisted Selection (Mas) Is An Impor-mentioning

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A Molecular Selection Index Method Based on Eigenanalysis

et al. 2008

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The traditional molecular selection index (MSI) employed in marker-assisted selection maximizes the selection response by combining information on molecular markers linked to quantitative trait loci (QTL) and phenotypic values of the traits of the individuals of interest. This study proposes an MSI based on an eigenanalysis method (molecular eigen selection index method, MESIM), where the first eigenvector is used as a selection index criterion, and its elements determine the proportion of the trait's contribution to the selection index. This article develops the theoretical framework of MESIM. Simulation results show that the genotypic means and the expected selection response from MESIM for each trait are equal to or greater than those from the traditional MSI. When several traits are simultaneously selected, MESIM performs well for traits with relatively low heritability. The main advantages of MESIM over the traditional molecular selection index are that its statistical sampling properties are known and that it does not require economic weights and thus can be used in practical applications when all or some of the traits need to be improved simultaneously. M ARKER-ASSISTED selection (MAS) is an impor-tant breeding tool in which molecular marker alleles linked to quantitative trait loci (QTL) that control phenotypic variables of important traits are selected. Marker-assisted selection can be more efficient than selecting individuals on the basis of phenotypic trait values. Progeny of specific progenitors can be selected on the basis of molecular markers as long as these are associated with breeding values of the traits under consideration. This is one form of MAS (Dekkers and Dentine 1991; Arus and Moreno-Gonzalez 1993). Another form of MAS is based on the molecular selection index (MSI) proposed by Lande and Thompson (1990). In MSI the selection response is maximized by combining information on molecular markers linked to QTL and the phenotypic values of the traits of interest.To construct an MSI, it is necessary to identify the linkage between the molecular marker and the QTL, the estimated effect of the QTL linked to the molecular marker (MQTL effect), and the combination of MQTL effects and phenotypic information that allows genotypes to be classified and selected using a selection index. The MQTL effects can be identified and estimated through the linkage disequilibrium that arises when crossing inbred lines or divergent populations Smith 1992, 1993;Xie and Xu 1998). The MSI depends on various factors, such as number and density of molecular markers associated with QTL, population size, trait heritability, additive genetic variances that can be explained by molecular markers, and precision of the estimated effect of gene substitution (Dekkers and Dentine 1991;Moreau et al. 2000).The MSI is an application of the selection index methodology proposed by Smith (1936), in which MQTL effects are incorporated. As proposed by Lande and Thompson (1990), the MSI performs a linear regression of phenotypi...

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Marker-assisted selection

Cited by 44 publications

References 82 publications

Genetic Characterization of two Eragrostis Species using AFLP and Morphological Traits

Genetic Characterization of two Eragrostis Species using AFLP and Morphological Traits

Genetic markers and the selection of quantitative traits in forage grasses

A Molecular Selection Index Method Based on Eigenanalysis

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