Marker-Assisted Breeding for Disease Resistance in Crop Plants

Collins, Paul J.; Zhang, Wen; Zhang, Shichen

doi:10.1007/978-3-319-94746-4_3

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…An economical approach to control clubroot disease is utilizing resistant materials and breeding resistant cultivars (Pang et al, 2018 ). With the development of molecular marker technology, MAS is an effective tool for screening for disease resistance (Collins et al, 2018 ). To date, a series of molecular markers have been developed and applied to select the new resistant germplasm resources (Kamei et al, 2010 ; Ueno et al, 2012 ; Wang et al, 2012 ; Hatakeyama et al, 2013 ; Zhang et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Locus RsCr6 Related to Clubroot Resistance in Radish (Raphanus sativus L.)

et al. 2022

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Clubroot is a devastating disease that causes substantial yield loss worldwide. However, the inheritance and molecular mechanisms of clubroot resistance during pathogen infection in radish remain largely unclear. In this study, we investigated the inheritance of clubroot resistance in the F2 population derived from crossing clubroot-resistant (CR) and clubroot-susceptible inbred lines “GLX” and “XNQ,” respectively. Genetic analysis revealed that a single dominant gene controlled the clubroot resistance of “GLX” with a Mendelian ratio of resistance and susceptibility of nearly 3:1. Bulked segregant analysis combined with whole-genome resequencing (BSA-seq) was performed to detect the target region of RsCr6 on chromosome Rs8. Linkage analysis revealed that the RsCr6 locus was located between two markers, HB321 and HB331, with an interval of approximately 92 kb. Based on the outcomes of transcriptome analysis, in the RsCr6 locus, the R120263140 and R120263070 genes with a possible relation to clubroot resistance were considered candidate genes. In addition, three core breeding materials containing the two reported quantitative trait loci (QTLs) and our novel locus RsCr6 targeting clubroot resistance were obtained using marker-assisted selection (MAS) technology. This study reveals a novel locus responsible for clubroot resistance in radishes. Further analysis of new genes may reveal the molecular mechanisms underlying the clubroot resistance of plants and provide a theoretical basis for radish resistance breeding.

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Section: Discussionmentioning

confidence: 99%

Identification of Novel Locus RsCr6 Related to Clubroot Resistance in Radish (Raphanus sativus L.)

et al. 2022

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“…Using molecular markers is most likely to increase the efficiency of the breeding process in cases where disease resistance is controlled by one or few genes, oligogenic traits, and those genes having a large effect on the resistance phenotype. In cases where disease resistance is controlled by many genes of small effect, genomic selection may be more efficient than MAS or phenotypic selection (Collins et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Single nucleotide polymorphism‐based haplotypes associated with Meloidogyne javanica resistance in Brazilian soybean germplasm

Vinholes¹,

Rosado

Borém

et al. 2021

Agronomy Journal

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The root-knot nematode (RKN) species Meloidogyne javanica (MJ) causes substantial root damage and yield loss in susceptible soybean [Glycine max (L.) Merr.] cultivars. In this study, a genome wide association study (GWAS) was undertaken to identify genomic regions controlling RKN resistance in a soybean associationmapping panel, using single nucleotide polymorphism (SNP) markers and haplotype information. This study was carried out with a sample (n = 124) from the Coodetec soybean gene bank, including some commercial Brazilian varieties.For the phenotypic evaluations MJ inoculum was obtained from roots of cultivar CD 206, a highly susceptible soybean used to maintain MJ for studies at Coodetec. The cultivars CD 208 and BRS CONQUISTA were used as resistant checks while CD 206, R7, and Nidera NA 5909 RG were used as susceptible checks and all 124 lines were genotyped with 6,000 SNP markers. MJ resistance determinants in the soybean panel were linked to SNPs with significant effects, with nine SNPs distributed along chromosomes 7, 13, and 20. The observed average MJ resistance in the group of soybean entries with haplotype GCT, ACC, ACT, and GCC in the markers Gm20_44446670_G_A, Gm13_29739984_C_A and Gm07_8166605_T_C, respectively, represents moderately resistant genotypes. Gm13_29739984_C_A and Gm07_8166605_T_C discriminated resistant and susceptible cultivars better than the marker Gm20_44446670_G_A. These results are particularly promising for soybeanbreeding programs, by not only identifying new resistance-associated haplotypes, but also suggesting new genetic mechanisms in RKN-resistance sources.

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“…Breeding activities are based on the collection and evaluation of genetic variation within a crop species, identification of superior alleles with a beneficial effect on the trait, promoting sexual recombination with elite genotypes, and selecting the individuals with the best phenotypic performance. In the last decades, selection assisted by molecular markers has moved selection from phenotype to DNA, with great advantages, depending on the trait, in terms of costs, time saving, and efficacy of selection [ 95 ]. Moreover, molecular selection can be done on seedling-stage materials, and it permits the enrichment of populations with heterozygous individuals by using codominant markers [ 96 ].…”

Section: Marker-assisted Selectionmentioning

confidence: 99%

Genomic Approaches to Identify Molecular Bases of Crop Resistance to Diseases and to Develop Future Breeding Strategies

Borrelli

Laidò

et al. 2021

IJMS

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Plant diseases are responsible for substantial crop losses each year and affect food security and agricultural sustainability. The improvement of crop resistance to pathogens through breeding represents an environmentally sound method for managing disease and minimizing these losses. The challenge is to breed varieties with a stable and broad-spectrum resistance. Different approaches, from markers to recent genomic and ‘post-genomic era’ technologies, will be reviewed in order to contribute to a better understanding of the complexity of host–pathogen interactions and genes, including those with small phenotypic effects and mechanisms that underlie resistance. An efficient combination of these approaches is herein proposed as the basis to develop a successful breeding strategy to obtain resistant crop varieties that yield higher in increasing disease scenarios.

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Marker-Assisted Breeding for Disease Resistance in Crop Plants

Cited by 5 publications

References 52 publications

Identification of Novel Locus RsCr6 Related to Clubroot Resistance in Radish (Raphanus sativus L.)

Identification of Novel Locus RsCr6 Related to Clubroot Resistance in Radish (Raphanus sativus L.)

Single nucleotide polymorphism‐based haplotypes associated with Meloidogyne javanica resistance in Brazilian soybean germplasm

Genomic Approaches to Identify Molecular Bases of Crop Resistance to Diseases and to Develop Future Breeding Strategies

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