Marine Myxobacteria as a Source of Antibiotics—Comparison of Physiology, Polyketide-Type Genes and Antibiotic Production of Three New Isolates of Enhygromyxa salina

Schäberle, Till F.; Goralski, Emilie; Neu, Edith; Erol, Özlem; Hölzl, Georg; Dörmann, Peter; Bierbaum, Gabriele; König, Gabriele M.

doi:10.3390/md8092466

Cited by 50 publications

(51 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1). The predominance of SCFAs in both genera (19,20) (20,43), but here, a predominance of straight-chain C 16:17c (42%), C 18:19c (29%), and C 16:0 (11%) was found. Although this study reproduced these findings with regard to detection of these FAs, the absence of quantitative data in previous studies prevents a true comparison.…”

Section: Resultsmentioning

confidence: 93%

Fatty Acid-Related Phylogeny of Myxobacteria as an Approach to Discover Polyunsaturated Omega-3/6 Fatty Acids

Garcia

Pistorius

Stadler³

et al. 2011

J Bacteriol

View full text Add to dashboard Cite

In an analysis of 47 aerobic myxobacterial strains, representing 19 genera in suborders Cystobacterineae, Nannocystineae, Sorangiineae, and a novel isolate, "Aetherobacter" SBSr008, an enormously diverse array of fatty acids (FAs) was found. The distribution of straight-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) supports the reported clustering of strains in the phylogenetic tree based on 16S rRNA genes. This finding additionally allows the prediction and assignment of the novel isolate SBSr008 into its corresponding taxon. Sorangiineae predominantly contains larger amounts of SCFA (57 to 84%) than BCFA. On the other hand, Cystobacterineae exhibit significant BCFA content (53 to 90%), with the exception of the genus Stigmatella. In Nannocystineae, the ratio of BCFA and SCFA seems dependent on the taxonomic clade. Myxobacteria could also be identified and classified by using their specific and predominant FAs as biomarkers. Nannocystineae is remarkably unique among the suborders for its absence of hydroxy FAs. After the identification of arachidonic (AA) FA in Phaselicystidaceae, eight additional polyunsaturated fatty acids (PUFAs) belonging to the omega-6 and omega-3 families were discovered. Here we present a comprehensive report of FAs found in aerobic myxobacteria. Gliding bacteria belonging to Flexibacter and Herpetosiphon were chosen for comparative analysis to determine their FA profiles in relation to the myxobacteria.

show abstract

Section: Resultsmentioning

confidence: 93%

Fatty Acid-Related Phylogeny of Myxobacteria as an Approach to Discover Polyunsaturated Omega-3/6 Fatty Acids

Garcia

Pistorius

Stadler³

et al. 2011

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…The addition of sodium [1,[2][3][4][5][6][7][8][9][10][11][12][13] C2]acetate to the culture medium of H. ochraceum led to the enhancement of 13 C-NMR signals of C-4 (δC 126.8, d, J = 56 Hz), C-5 (δC 125.5, d, J = 56 Hz) and C-10 (δC 112.4, s), corresponding one intact acetate unit and one acetate-derived terminal methylene in the haliamide structure ( Figure 3). The second feeding experiment with sodium [1-13 C]propionate gave two isotope-labeled carbons of C-7 (δC 47.4) and C-9 (δC 144.2).…”

Section: Identification Of the Biosynthetic Precursorsmentioning

confidence: 99%

“…Although long been regarded as terrestrial microorganisms, several halophilic myxobacterial strains have been noticed recently [4][5][6][7][8] and found to be excellent producers of novel secondary metabolites, such as polyketides (PKs), non-ribosomal peptides (NRPs) and their hybrids. A recent report suggested that the polyketide synthase (PKS) gene sequences in marine-derived myxobacterial isolates are highly novel (all of the sequences were less than 70% similar to the known ones) [9].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid Metabolite from the Marine Myxobacterium Haliangium ochraceum

et al. 2016

View full text Add to dashboard Cite

Myxobacteria of marine origin are rare and hard-to-culture microorganisms, but they genetically harbor high potential to produce novel antibiotics. An extensive investigation on the secondary metabolome of the unique marine myxobacterium Haliangium ochraceum SMP-2 led to the isolation of a new polyketide-nonribosomal peptide hybrid product, haliamide (1). Its structure was elucidated by spectroscopic analyses including NMR and HR-MS. Haliamide (1) showed cytotoxicity against HeLa-S3 cells with IC 50 of 12 µM. Feeding experiments were performed to identify the biosynthetic building blocks of 1, revealing one benzoate, one alanine, two propionates, one acetate and one acetate-derived terminal methylene. The biosynthetic gene cluster of haliamide (hla, 21.7 kbp) was characterized through the genome mining of the producer, allowing us to establish a model for the haliamide biosynthesis. The sulfotransferase (ST)-thioesterase (TE) domains encoded in hlaB appears to be responsible for the terminal alkene formation via decarboxylation.

show abstract

“…They predominantly inhabit terrestrial but their presence is also reported in aquatic environment and they have been isolated from tree bark and animal dung. The genomes of the Myxobacteria have high GC content of ~70% [13,14]. Myxobacteria have unique characteristics among the bacteria as they show coordinated motility, predation and produce multicellular fruiting bodies under nutrient limited condition [15].…”

Section: Introductionmentioning

confidence: 99%

Prediction of Metal Ion Binding Site in Truncated Globin of Myxococcus xanthus DK1622 in Homologous Model

Kumar¹

2017

MOJPB

View full text Add to dashboard Cite

Globins are heme containing proteins with the capability to bind oxygen reversibly. All globins can be classified as M (myoglobin-like), S (sensor) and T (truncated) globins; among them, M and S globins have a typical 3/3 α-helical globin fold and T globins have a 2/2 α-helical fold. Till date, all the myxobacterial genomes available in NCBI data base harbor one or more genes for globins but none of the globins from this group of bacteria have been characterized as yet. In this study, in-silico analysis and homology modeling approach was exploited to explore the physiochemical properties and 3-D structure of trHbN (group I T-globin) of Myxococcus xanthus DK1622, a fruiting myxobacteria. After generating the 3-D model, assessment of the structure and stereo-chemical analysis was carried out using different evaluation and validation tools. As homologs of the trHbN exist in dimeric as well as monomeric form, dimerization studies were performed and it was observed that dimeric form of the protein is more stable as compared to the monomer. Further, heme group was appended to the model structure of trHbN of M. xanthus by Patchdock web server and model-heme complex was further refined by Firedock. The 3-D model structure will enhance the understanding for functional analysis of experimentally derived crystal structure.

show abstract

Marine Myxobacteria as a Source of Antibiotics—Comparison of Physiology, Polyketide-Type Genes and Antibiotic Production of Three New Isolates of Enhygromyxa salina

Cited by 50 publications

References 26 publications

Fatty Acid-Related Phylogeny of Myxobacteria as an Approach to Discover Polyunsaturated Omega-3/6 Fatty Acids

Fatty Acid-Related Phylogeny of Myxobacteria as an Approach to Discover Polyunsaturated Omega-3/6 Fatty Acids

Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid Metabolite from the Marine Myxobacterium Haliangium ochraceum

Prediction of Metal Ion Binding Site in Truncated Globin of Myxococcus xanthus DK1622 in Homologous Model

Contact Info

Product

Resources

About