Marine genomics goes viral

Koch, Linda

doi:10.1038/nrg.2016.130

Cited by 1 publication

(2 citation statements)

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“…This finding suggested the significance of the extracellular virus community and opened a new era in the field of environmental virology. Advances in high‐throughput sequencing technologies in the last decade have enabled us to identify the genetic diversity of environmental DNA virus particles (Breitbart et al, ; Edwards & Rohwer, ; Koch, ; Suttle, ) and have revealed that DNA viruses are the most abundant entities across all habitats and a major reservoir of genetic diversity (Paez‐Espino et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Unveiling the RNA virosphere associated with marine microorganisms

Urayama

Takaki

Nishi

et al. 2018

Molecular Ecology Resources

100

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The study of extracellular DNA viral particles in the ocean is currently one of the most advanced fields of research in viral metagenomic analysis. However, even though the intracellular viruses of marine microorganisms might be the major source of extracellular virus particles in the ocean, the diversity of these intracellular viruses is not well understood. Here, our newly developed method, referred to herein as fragmented and primer ligated dsRNA sequencing (flds) version 2, identified considerable genetic diversity of marine RNA viruses in cell fractions obtained from surface seawater. The RNA virus community appears to cover genome sequences related to more than half of the established positive-sense ssRNA and dsRNA virus families, in addition to a number of unidentified viral lineages, and such diversity had not been previously observed in floating viral particles. In this study, more dsRNA viral contigs were detected in host cells than in extracellular viral particles. This illustrates the magnitude of the previously unknown marine RNA virus population in cell fractions, which has only been partially assessed by cellular metatranscriptomics and not by contemporary viral metagenomic studies. These results reveal the importance of studying cell fractions to illuminate the full spectrum of viral diversity on Earth.

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Section: Introductionmentioning

confidence: 99%

“…in the last decade have enabled us to identify the genetic diversity of environmental DNA virus particles (Breitbart et al, 2002;Edwards & Rohwer, 2005;Koch, 2016;Suttle, 2016) and have revealed that DNA viruses are the most abundant entities across all habitats and a major reservoir of genetic diversity (Paez-Espino et al, 2016).…”

mentioning

confidence: 99%