Maresin 1 improves the Treg/Th17 imbalance in rheumatoid arthritis through miR-21

Jin, Shengwei; Chen, Huaijun; Li, Yongsheng; Zhong, Hao; Sun, Weiwei; Wang, Jianmin; Zhang, Tingting; Ma, Jinglan; Yan, Songfan; Zhang, Jiangang; Tian, Qingqing; Yang, Xinyu; Wang, Jianguang

doi:10.1136/annrheumdis-2018-213511

Cited by 140 publications

(87 citation statements)

References 37 publications

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“…In FLS of RA patients, miRNA-23b can regulate arthritic inflammation by inhibiting the NF-κB signal pathway [16]. Furthermore, SPMs can regulate the Treg/Th17 imbalance in CIA mice by upregulating miR-21 [17]. These studies indicate that RvD1 is apt to exert its biological functions through microRNA.…”

Section: Introductionmentioning

confidence: 85%

Resolvin D1 suppresses pannus formation via decreasing connective tissue growth factor caused by upregulation of miRNA-146a-5p in rheumatoid arthritis

Sun

Han

et al. 2020

Arthritis Res Ther

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Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by inflammation and joint stiffness, finally leading to tissue destruction. Connective tissue growth factor (CTGF) is a critical factor in RA progression, which promotes fibroblast-like synoviocyte (FLS) proliferation, pannus formation, and the damage of cartilage as well as bone. Resolvin D1 (RvD1) can promote inflammation resolution in acute inflammatory diseases, and recently, effects of RvD1 on chronic inflammatory diseases also attracted attention. This study aimed to examine the effect of RvD1 on pannus formation in RA and the underlying mechanism. Methods: Serum levels of RvD1 and CTGF were determined in RA patients and healthy persons by UPLC-MS/MS and ELISA respectively. The levels of CTGF and inflammatory factors were assessed by qRT-PCR and ELISA. MicroRNA expression profile was determined by miRNA microarray. The effects of CTGF, RvD1, and miR-146a-5p on angiogenesis were evaluated with tube formation and chick chorioallantoic membrane (CAM) assays. Collagen-induced arthritis (CIA) mice were constructed to detect the effects of RvD1 and miR146a-5p on RA. STAT3 activation was determined by Western blotting. Results: RvD1 levels decreased while CTGF levels increased in RA patients' serum, and an inverse correlation of the concentrations of RvD1 and CTGF in the serum of RA patients was synchronously observed. In CIA mice, RvD1 suppressed angiopoiesis and decreased the expression of CTGF. Simultaneously, RvD1 significantly decreased CTGF and proinflammation cytokines levels in RA FLS. Furthermore, CTGF suppressed angiopoiesis and RvD1 inhibited the proliferation and migration of RA FLS and angiopoiesis. MiRNA microarray and qRT-PCR results showed that RvD1 upregulated miRNA-146a-5p. The transfection experiments demonstrated that miRNA-146a-5p could decrease inflammatory factors and CTGF levels. Moreover, miRNA-146a-5p decreased the proliferation of FLS and angiogenesis in vivo. MiRNA-146a-5p also suppressed angiogenesis and downregulated the expression of CTGF in CIA mice. Finally, Western blot results revealed that miRNA-146a-5p inhibited the activation of STAT3.

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Section: Introductionmentioning

confidence: 85%

Resolvin D1 suppresses pannus formation via decreasing connective tissue growth factor caused by upregulation of miRNA-146a-5p in rheumatoid arthritis

Sun

Han

et al. 2020

Arthritis Res Ther

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“…In T lymphocyte progenitors, such mechanism would drive differentiation of T lymphocytes towards the anti‐inflammatory regulatory T cells phenotype (Melnik & Schmitz, ). This would, therefore, explain the beneficial role of milk EVs observed on rheumatoid arthritis (Arntz et al., ) for which a lymphocyte Th17/Treg balance is of prime importance and can be modulated by microRNAs (Jin et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 97%

Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

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MicroRNAs are small noncoding RNAs responsible for regulating 40% to 60% of gene expression at the posttranscriptional level. The discovery of circulating microRNAs in several biological fluids opened the path for their study as biomarkers and long‐range cell‐to‐cell communication mediators. Their transfer between individuals in the case of blood transfusion, for example, and their high enrichment in milk have sparked the interest for microRNA transfer through diet, especially from mothers to infants during breastfeeding. The extension of such paradigm led to the study of milk microRNAs in the case of cow or goat milk consumption in adults. Here we provide a comprehensive critical review of the key findings surrounding milk microRNAs in human, cow, and goat milk among other species. We discuss the data on their biological properties, their use as disease biomarkers, their transfer between individuals or species, and their putative or verified functions in health and disease of infants and adult consumers. This work is based on all the literature available and integrates all the results, theories, debates, and validation studies available so far on milk microRNAs and related areas of investigations. We critically discuss the limitations and outline future aspects and avenues to explore in this rapidly growing field of research that could impact public health through infant milk formulations or new therapies. We hope that this comprehensive review of the literature will provide insight for all teams investigating milk RNAs’ biological activities and help ensure the quality of future reports.

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“…Maresin (MaR) family is a newly described macrophagederived mediator of inflammation resolution [7]. MaR1 is the first reported member of this family and shows broad antiinflammatory actions under physiologic conditions in endothelial cells and vascular smooth muscle cells [8], human periodontal ligament cells [9], and has been reported to have benefits on multiple diseases, such as acute pancreatitis [10], neurocognitive disorders [11], and inflammatory arthritis [12]. More important, recent studies also revealed that MaR1 play roles in obesity [13] and nonalcoholic steatohepatitis [14], indicating a strong association between MaR1 and metabolic diseases.…”

Section: Introductionmentioning

confidence: 99%

Decreased Plasma Maresin 1 Concentration Is Associated with Diabetic Foot Ulcer

Tian

Huang

et al. 2020

Mediators of Inflammation

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Aims. To assess the maresin 1 (MaR1) contents in type 2 diabetic patients with or without diabetic foot ulcer and to analyze the association of MaR1 concentrations with several metabolism-related parameters. Methods. Plasma MaR1 concentrations were analyzed in 96 subjects with normal glucose tolerant (NC, n=43), type 2 diabetes (T2DM, n=40), or diabetic foot ulcer (DFU, n=13). The intravenous glucose tolerance test (IVGTT) and biochemical parameters were measured in all participants. Results. Plasma MaR1 concentrations were significant decreased in type 2 diabetes patient with or without DFU compared with NC (both P<0.001) and were lowest in DFU patients among these 3 groups. (DFU vs. T2DM, P<0.05). Plasma MaR1 concentrations were negatively correlated with BMI, waist circumference (Wc), waist hip ratio (WHR), systolic blood pressure (SBP), diastolic blood pressure (DBP), LDL-c, FPG, 2hPG, HbA1c, and homeostasis model assessment for insulin resistance (HOMA-IR) (all P<0.05) and were positively correlated with HDL-c, acute insulin response (AIR), area under the curve of the first-phase (0-10 min) insulin secretion (AUC), and homeostasis model assessment for beta-cell function (HOMA-β) (all P<0.05). After adjusting for age and sex, Wc, WHR, TG, FPG, 2hPG, HbA1c, HOMA-IR, AIR, AUC, and HOMA-β remain statistically significant (all P<0.05). Conclusions. Plasma MaR1 concentration were decreased in T2DM with or without DFUs and were the lowest in DFU patients. The decreased plasma MaR1 strongly associated with obesity, impaired glucose and lipid metabolism, reduced first-phase of glucose-stimulated insulin secretion, and enhanced insulin resistance.

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Maresin 1 improves the Treg/Th17 imbalance in rheumatoid arthritis through miR-21

Abstract: MaR1 is a therapeutic target for RA, likely operating through effects on the imbalanced Treg/Th17 ratio found in the disease.

Cited by 140 publications

References 37 publications

Resolvin D1 suppresses pannus formation via decreasing connective tissue growth factor caused by upregulation of miRNA-146a-5p in rheumatoid arthritis

Resolvin D1 suppresses pannus formation via decreasing connective tissue growth factor caused by upregulation of miRNA-146a-5p in rheumatoid arthritis

Milk MicroRNAs in Health and Disease

Decreased Plasma Maresin 1 Concentration Is Associated with Diabetic Foot Ulcer

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