Marek's disease virus EcoRI-Q gene (meq) and a small RNA antisense to ICP4 are abundantly expressed in CD4+ cells and cells carrying a novel lymphoid marker, AV37, in Marek's disease lymphomas.

Ross, Norman; O’Sullivan, Gerald C.; Rothwell, Christopher; Smith, G.; Burgess, Shane C.; Rennie, M.; Lee, L F; Davison, T.F.

doi:10.1099/0022-1317-78-9-2191

Cited by 69 publications

(42 citation statements)

References 33 publications

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“…The Meq protein is a transactivating bZIP protein homologous to fos and jun oncogene products, and has an anti-apoptotic activity [12,14,15]. The Meq protein is consistently expressed in the vast majority of MD-transformed cell lines and CD4+ T cells obtained from lymphomas [24]. Oligonucleotides and RNA complementary to the meq gene can prevent the growth of MDV cell lines [29], and overexpression of Meq in rat-2 fibroblasts by recombinant murine retrovirus leads to transformation of the cells [12,15].…”

Section: Discussionmentioning

confidence: 99%

The Detection of the meq Gene in Chicken Infected with Marek's Disease Virus Serotype 1.

et al. 2002

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ABSTRACT. In the genome of strains of very virulent Marek's disease virus serotype 1(vvMDV1), such as Md5 and RB1B, the meq open reading frame (ORF) encoding a 339-amino-acid bZIP protein, is present, while a slightly longer meq ORF, termed as L-meq, in which a 180-bp sequence is inserted into the meq ORF is found in other strains of MDV1, such as CVI988/R6 and attenuated JM. When chickens were infected with vvMDV1 strains and the meq gene was amplified by nested polymerase chain reaction (PCR), the meq gene was detected throughout the experimental period for 7 weeks post inoculation (pi). However, the L-meq gene was also detected at 3 to 5 weeks and 3 to 4 weeks pi. in Md5-infected and RB1B-infected chickens, respectively. In the case of chickens infected with an attenuated MDV1, the JM strain, the L-meq gene was detected at 2 to 7 weeks pi., and the meq gene was also detected at 2 to 6 weeks pi. Both L-meq and meq genes were detected in chickens infected with an attenuated nononcogenic vaccine strain of MDV1 (CVI988/R6), throughout the experimental period. Though quantitative PCR was not performed, a larger amount of the PCR products corresponding to the L-meq than the meq gene was amplified from chickens infected with JM or CVI988/R6. These results suggest that a dynamic population shift between the MDV subpopulations displaying meq and L-meq genes occurs in chickens during the course of MDV infection. Since the MDV subpopulation that displays the L-meq gene only displays it during the latent phase, the L-meq and its gene product, if any, might contribute to the maintenance of the MDV latency.

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Section: Discussionmentioning

confidence: 99%

The Detection of the meq Gene in Chicken Infected with Marek's Disease Virus Serotype 1.

et al. 2002

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“…The meq antigen has been reported to be expressed in a variable proportion of MDV-transformed lymphoblasts in both cell lines and natural tumors. 31 The CD4ϩ CD8Ϫ lymphoblast is a principal target cell population for MDV transformation. 32 The present results suggest that late proliferative lesions in the CNS contain at least some MDV-transformed cells.…”

Section: Discussionmentioning

confidence: 99%

Marek's Disease Virus Infection in the Brain: Virus Replication, Cellular Infiltration, and Major Histocompatibility Complex Antigen Expression

Gimeno¹,

Witter²,

Hunt³

et al. 2001

Vet Pathol

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Abstract. Marek's disease virus (MDV) infection in the brain was studied chronologically after inoculating 3-week-old chickens of two genetic lines with two strains of serotype 1 MDV representing two pathotypes (v and vvϩ). Viral replication in the brain was strongly associated with the development of lesions. Three viral antigens (pp38, gB, and meq) were detected in the brain of infected chickens. Marked differences between v and vvϩ pathotypes of MDV were identified for level of virus replication, time course of brain lesions, and expression of major histocompatibility complex (MHC) antigens. Two pathologic phenomena (inflammatory and proliferative) were detected in the brain of chickens inoculated with vvϩMDV, but only inflammatory lesions were observed in those inoculated with vMDV. Inflammatory lesions, mainly composed of macrophages, CD4ϩ T cells, and CD8ϩ T cells, started at 6-10 days postinoculation (dpi) and were transient. Proliferative lesions, characterized by severe infiltrates of CD4ϩCD8Ϫ T cells (blasts), started at 19-26 dpi and persisted. Expression of MHC antigens in endothelial cells and infiltrating cells within the brain was influenced by MDV infection. Upregulation of MHC class II antigen occurred in all treatment groups, although it was more severe in those inoculated with vvϩMDV. MHC class I antigen was downregulated only in those groups inoculated with vvϩMDV. These results enhance our understanding of the nature and pattern of MDV infection in the brain and help to explain the neurovirulence associated with highly virulent MDV.

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“…after MDV infection, and AV37 was thought to perhaps be associated with MHC class I and II expression, thus suggesting a possible role in activation of the cells. Ross et al (1997) found that expression of AV37 in CD4 + cells correlated with expression of a putative MDV oncogene, meq, (Jones et al, 1992) and small RNAs antisense to ICP4 (SAR) (Li et al, 1994;Xie et al, 1996;Cantello et al, 1997). T-cells do not enjoy an invariable monopoly on susceptibility to transformation since both B-and T-cell lymphoblastoid cell lines have been derived from MDV-induced lymphomas from turkeys (Nazerian & Sharma, 1985;Powell et al, 1984).…”

Section: Target Cellsmentioning

confidence: 99%

“…They include: a 1.8-kb family of RNAs which are present in oncogenic viruses, but are truncated in attenuated MDV variants (Maotani et al, 1986;Bradley et al, 1989;Peng et al, 1992); a 38-kDa phosphoprotein (pp38) found in cell lines and tumour cells (Cui-ef al, 1990); the MDV ICP4 homologue (Anderson et al, 1992), and a group of latency-related RNAs which map antisense to the ICP4 gene and are found in MD lymphoblastoid cell lines and in productively infected chicken embryo fibroblasts (Li et al, 1994;McKie et al, 1995;Cantello et al, 1997); a basic-leucine zipper gene, meq, which is expressed in all MD cell lines and MDV-induced tumours (Jones et al, 1992); and AV37 (Burgess et al, 1996;Kaiser et al, 1996;Ross et al, 1996Ross et al, , 1997, a novel antigen found on infected and transformed cells. The evidence that these various candidate genes and proteins are involved in oncogenesis with MDV is incomplete and, in some cases, very tenuous or completely absent.…”

Section: Differing Rates Of Integration Of Viral Dnamentioning

confidence: 99%

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Lymphomagenesis in Marek's disease

Calnek

1998

Avian Pathology

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Marek's disease virus EcoRI-Q gene (meq) and a small RNA antisense to ICP4 are abundantly expressed in CD4+ cells and cells carrying a novel lymphoid marker, AV37, in Marek's disease lymphomas.

Abstract: Mature lymphomas produced in Rhode Island

Cited by 69 publications

References 33 publications

The Detection of the meq Gene in Chicken Infected with Marek's Disease Virus Serotype 1.

The Detection of the meq Gene in Chicken Infected with Marek's Disease Virus Serotype 1.

Marek's Disease Virus Infection in the Brain: Virus Replication, Cellular Infiltration, and Major Histocompatibility Complex Antigen Expression

Lymphomagenesis in Marek's disease

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