Mapping of somatostatin-28 (1–12) in the alpaca diencephalon

Coveñas, Rafael; Mangas, A.; Medina, L.E.; Sánchez, Manuel Lisardo; Aguilar, Luís Fuentes; Dı́az-Cabiale, Zaida; Narváez, José Ángel

doi:10.1016/j.jchemneu.2011.06.006

Cited by 15 publications

(77 citation statements)

References 47 publications

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“…In this sense, the distribution of CGRP has been reported in the alpaca brainstem (de Souza et al, 2008) and the colocalization of this neuropeptide with tyrosine hydroxylase has been also studied in the same central nervous system region of this ungulate (Marcos et al, 2011). Comparing the distribution of immunoreactive cell bodies containing the above-mentioned neuropeptides, it should be remarked that both the distribution and number of cell bodies containing CGRP are considerably higher than those observed for perikarya containing leucine-enkephalin or somatostatin-28 (1-12) (de Souza et al, 2007(de Souza et al, , 2008Coveñ as et al, 2011). In all cases, these studies were carried out in control animals; that is, in alpacas not treated with colchicine.…”

Section: Introductionmentioning

confidence: 91%

“…The perfusion of the animals, the tissue preparation and the histological and immunocytochemical procedures used in this work have been published previously (see de Souza et al, 2008;Coveñ as et al, 2011), as well as the characteristics of the polyclonal CGRP antiserum (obtained at the laboratory of Professor Gé rard Tramu, University of Bordeaux I, France) and the specificity of the immunostaning observed (see de Souza et al, 2008). In brief, the anti-CGRP was raised in rabbits against immunogens prepared by coupling the whole synthetic CGRP peptide to human serum albumin with glutaraldehyde.…”

Section: Tissue Preparation Immunocytochemistry and Specificity Of Tmentioning

confidence: 99%

“…Mapping was carried out following the frontal planes of the alpaca diencephalon that appear in a recently published article addressing the distribution of somatostatin-28 (1-12) in the diencephalon of the alpaca (see Coveñ as et al, 2011). Moreover, the brain atlas of Lama glama (available from the Mammalian Brain Collections of the University of WI, Madison, USA) was used.…”

Section: Mappingmentioning

confidence: 99%

“…This involved viewing the sections under illumination by light at constant magnification with reference to photographs of a defined series of densities for immunoreactive fibers (high, moderate, low) established previously (de Souza et al, 2007(de Souza et al, , 2008Coveñ as et al, 2011). Moreover, as previously published, immunoreactive fibers were considered short (<90 mm), medium (90-120 mm) or long in length (>120 mm) and cell bodies were considered small (diameter below 15 mm); medium-sized (15-25 mm), and large (above 25 mm) (see Coveñ as et al, 2011). The size of the cell body was measured when the nucleus was visible.…”

Section: Mappingmentioning

confidence: 99%

“…In South America camelid research has been focused in general on their reproductive mechanisms (Bravo et al, 1996;Correa et al, 1997;Ratto et al, 1997Ratto et al, , 2005Ratto et al, , 2006 due to the economic importance of their wool, although recently the distribution of three neuropeptides belonging to different families of peptides (leucine-enkephalin, calcitonin gene-related peptide (CGRP), somatostatin-28 (1-12)) and tyrosine hydroxylase has been reported in the alpaca central nervous system (brainstem and diencephalon) (de Souza et al, 2007(de Souza et al, , 2008Coveñ as et al, 2011;Marcos et al, 2011). In this sense, the distribution of CGRP has been reported in the alpaca brainstem (de Souza et al, 2008) and the colocalization of this neuropeptide with tyrosine hydroxylase has been also studied in the same central nervous system region of this ungulate (Marcos et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Mapping of CGRP in the alpaca diencephalon

Coveñas

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et al. 2012

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Section: Introductionmentioning

confidence: 91%

Section: Tissue Preparation Immunocytochemistry and Specificity Of Tmentioning

confidence: 99%

Section: Mappingmentioning

confidence: 99%

Section: Mappingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mapping of CGRP in the alpaca diencephalon

Coveñas

Sánchez

Mangas

et al. 2012

Journal of Chemical Neuroanatomy

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Immunohistochemical Localization of Somatostatin in the Brain of Chinese Alligator Alligator sinensis

Wang

Zhang

et al. 2016

The Anatomical Record

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In this study, the regional distribution and histological localization of somatostatin (SS) immunoreactive (IR) perikarya and fibers was investigated for the first time in the brain of adult Chinese alligator by immunohistochemical method. The results showed SS-IR perikarya and fibers were widely distributed in various parts of the brain except for olfactory bulbs. In the telencephalon, SS-IR perikarya were predominantly located in the cellular layer and deep plexiform layer of dorsomedial and medial cortex, less in the dorsal and lateral cortex, while SS-IR fibers were found in all layers of the cerebral cortex. SS-IR perikarya and fibers were also detected in the dorsal ventricular ridge, hippocampus cortex, accessory olfactory bulb nuclearus, lenticular nucleus, and caudate nucleus. In the diencephalon, SS-IR perikarya and fibers were mainly present in supraoptic nucleus, paraventricular nucleus of hypothalamus, recessus infundibular nucleus, median eminence, the pineal gland and pituitary gland, in which the IR-fibers were abundant, appearing dot-shaped and varicosity-like. In the mesencephalon, they were present in tectum cortex, ependyma of cerebral aqueduct and the periaqueductal grey matter. Additionally, they were also detected in Purkinje's cellular layer of cerebellum, in the reticularis nucleus and raphe nucleus of medulla oblongata. The distribution pattern of SS-IR perikarya and fibers in the brain of Chinese alligator is generally similar to that reported in other reptiles, but also has some specific features. The wide distribution indicated that SS might be a neurotransmitter or neuromodulator which acts on many kinds of target cells with a wide range of physiological functions. Anat Rec, 300:507-519, 2017. © 2016 Wiley Periodicals, Inc.

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Distribution of CGRP in the minipig brainstem

Sánchez

Vecino

Coveñas

2014

Microscopy Res & Technique

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For the first time, an in-depth study has been made of the distribution of fibers and cell bodies containing calcitonin gene-related peptide (CGRP) in the minipig brainstem using an indirect immunoperoxidase technique. The animals studied were not treated with colchicine. Cell bodies containing CGRP were found in 20 nuclei/regions of the brainstem. These perikarya were located in somatomotor, brachiomotor and raphae nuclei, nucleus ambiguus, substantia nigra, nucleus reticularis tegmenti pontis, nucleus prepositus hypoglossi, nuclei olivaris inferior and superior, nuclei pontis, formatio reticularis, nucleus dorsalis tegmenti of Gudden, and in the nucleus reticularis lateralis. Fourteen of the 20 brainstem nuclei showed a high density of immunoreactive cell bodies. In comparison with other species, the minipig, together with the rat, show the most widespread distribution of cell bodies containing CGRP in the mammalian brainstem. Immunoreactive fibers were also observed in the brainstem. However, in the minipig brainstem the density of these fibers is low, as in many brainstem nuclei only single immunoreactive fibers were observed. A high density of immunoreactive fibers was only observed in the pars caudalis of the nucleus tractus spinalis nervi trigemini and in the nucleus ventralis tegmenti of Gudden. According to the observed anatomical distribution of the immunoreactive structures containing CGRP, the peptide could be involved in motor, somatosensory, gustative, and autonomic mechanisms.

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Mapping of somatostatin-28 (1–12) in the alpaca diencephalon

Cited by 15 publications

References 47 publications

Mapping of CGRP in the alpaca diencephalon

Mapping of CGRP in the alpaca diencephalon

Immunohistochemical Localization of Somatostatin in the Brain of Chinese Alligator Alligator sinensis

Distribution of CGRP in the minipig brainstem

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