Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy

Bukara, Katarina; Jovanić, Svetlana Z.; Drvenica, Ivana; Stančić, Ana; Ilić, Vesna; Rabasović, Mihailo D.; Pantelić, Dejan; Jelenković, Branislav; Bugarski, Branko; Krmpot, Aleksandar J.

doi:10.1117/1.jbo.22.2.026003

Cited by 21 publications

(20 citation statements)

References 46 publications

(59 reference statements)

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“…At the molecular level, field distortions surround the paramagnetic heme iron. Furthermore, distribution of Hb (and therefore deoxyhemoglobin) within the erythrocyte is not uniform, as the Hb protein occupies a ring at the periphery of the cell . Spin dephasing induced by these nonuniform field distortions increases the erythrocyte relaxation rate, R 2,ery .…”

Section: Discussionmentioning

confidence: 99%

Human umbilical cord blood relaxation times and susceptibility at 3 T

Portnoy

Milligan

Seed

et al. 2017

Magnetic Resonance in Med

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Section: Discussionmentioning

confidence: 99%

Human umbilical cord blood relaxation times and susceptibility at 3 T

Portnoy

Milligan

Seed

et al. 2017

Magnetic Resonance in Med

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“…Second harmonic generation imaging of colon tissue samples. The images of collagen fibers in the label-free human colon tissue samples were obtained using an original lab frame nonlinear laser-scanning microscope 47,48 . For second harmonic generation (SHG) imaging of collagen fibers following experimental setup for nonlinear laser scanning microscope (NLM) was used 22 : The tunable mode-locked Ti:sapphire laser (Coherent, Mira 900) has been source of the infrared femtosecond pulses.…”

Section: Methodsmentioning

confidence: 99%

Altered organization of collagen fibers in the uninvolved human colon mucosa 10 cm and 20 cm away from the malignant tumor

Despotović

Milićević

Krmpot

et al. 2020

Sci Rep

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Remodelling of collagen fibers has been described during every phase of cancer genesis and progression. Changes in morphology and organization of collagen fibers contribute to the formation of microenvironment that favors cancer progression and development of metastasis. However, there are only few data about remodelling of collagen fibers in healthy looking mucosa distant from the cancer. Using SHG imaging, electron microscopy and specialized softwares (CT-FIRE, CurveAlign and FiberFit), we objectively visualized and quantified changes in morphology and organization of collagen fibers and investigated possible causes of collagen remodelling (change in syntheses, degradation and collagen cross-linking) in the colon mucosa 10 cm and 20 cm away from the cancer in comparison with healthy mucosa. We showed that in the lamina propria this far from the colon cancer, there were changes in collagen architecture (width, straightness, alignment of collagen fibers and collagen molecules inside fibers), increased representation of myofibroblasts and increase expression of collagen-remodelling enzymes (LOX and MMP2). Thus, the changes in organization of collagen fibers, which were already described in the cancer microenvironment, also exist in the mucosa far from the cancer, but smaller in magnitude. Extracellular matrix (ECM) is no longer considered as an inert substrate, a three-dimensional network which only "fills the spaces" between cells and provide mechanical support 1,2. Today, ECM is known to be a complex and dynamic structure, whose chemical and biophysical properties affect cell adhesion 3 , proliferation 4 morphology 5 , migration 6 , regulate tissue morphogenesis 7,8 and fluid volume in tissues 9. The most abundant component of ECM in the lamina propria of the colon mucosa is type I collagen. Remodelling of collagen fibers has been described in almost every solid cancer, including colorectal cancer. During tumor formation and progression, collagen remodelling is constantly carried out: degradation, synthesis, cross-linking of fibers, change of fiber orientation, and interaction of cells of the innate and acquired immune system with collagen fibers 10,11. Changes in morphology, representation, and organization of collagen fibers contribute to the formation of the microenvironment that favors tumor progression, primarily through its effect on cell migration and polarization 12. Also, remodelling of collagen fibers on premetastatic sites is of great importance in determination of survival and growth of disseminated cancer cells, and thus, formation of metastasis 13,14. Remodelling of collagen fibers may be a result of changes in synthesis, degradation or cross-linking. Main cells responsible for synthesis of collagen in colon mucosa are fibroblasts and myofibroblasts. The most important enzymes for degradation of collagen fibers are matrix metalloproteinases (MMPs). It has been shown that expression of MMP2 and MMP9 is increased in colorectal cancer and influences its progression and

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“…The homemade nonlinear laser-scanning microscope used in this study has been described in detail elsewhere. [42,43] The SHG filter central wavelength was 420nm (FB420-10, Thorlabs) for Ti:Sapphire laser when tuned at 840nm. The SHG excitation wavelength and filter were selected so that fluorescence leakage to SHG channel is minimal.…”

Section: Nonlinear Laser Scanning Microscopymentioning

confidence: 99%

Micromechanical imaging of dentin with Brillouin microscopy

et al. 2020

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The physical structure of teeth can be altered by diet, age or diseases such as caries or sclerosis. It is of utmost importance to characterize the mechanical properties to predict and understand tooth decay, to design restorative dental procedure, and to investigate the tribological behavior of teeth. Yet, existing imaging techniques are unable to reveal the micromechanics of the tooth, in particular at tissue interfaces. Here we developed a microscope based on Brillouin light scattering (BLS) to probe mechanical changes in tooth tissues. BLS is an inelastic process that uses the scattering of light by acoustic waves in the GHz range. Our microscope thus reveals the mechanical properties at a submicrometer scale without contact to the sample. BLS signals show significant differences between healthy tissues and pathological lesions, and allow delineating precisely destructed dentin. We also show maps of the sagittal and transversal planes of healthy tubular dentin that reveal its anisotropic microstructure with a 1 µm resolution, several orders of magnitude below previous reports. Our observations indicate that the collagen-based matrix of dentine is the main load bearing structure, which can be thought of as a fiber-reinforced composite. In the vicinity of polymeric tooth-filling materials, we observed fingering of the adhesive complex into the opened tubules of healthy dentine. The ability to probe the quality of this interfacial layer could lead to innovative designs of biomaterials used for dental restorations in contemporary adhesive dentistry, and would have direct repercussions on the decision-making during clinical work.

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Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy

Cited by 21 publications

References 46 publications

Human umbilical cord blood relaxation times and susceptibility at 3 T

Human umbilical cord blood relaxation times and susceptibility at 3 T

Altered organization of collagen fibers in the uninvolved human colon mucosa 10 cm and 20 cm away from the malignant tumor

Micromechanical imaging of dentin with Brillouin microscopy

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