Mapping and identification of essential gene functions on the X chromosome of Drosophila

Abstract

The Drosophila melanogaster genome consists of four chromosomes that contain 165 Mb of DNA, 120 Mb of which are euchromatic. The two Drosophila Genome Projects, in collaboration with Celera Genomics Systems, have sequenced the genome, complementing the previously established physical and genetic maps. In addition, the Berkeley Drosophila Genome Project has undertaken large-scale functional analysis based on mutagenesis by transposable P element insertions into autosomes. Here, we present a large-scale P element insertion screen for vital gene functions and a BAC tiling map for the X chromosome. A collection of 501 X-chromosomal P element insertion lines was used to map essential genes cytogenetically and to establish short sequence tags (STSs) linking the insertion sites to the genome. The distribution of the P element integration sites, the identified genes and transcription units as well as the expression patterns of the P-element-tagged enhancers is described and discussed.

Enhancer trap infidelity inDrosophila optomotor-blind

“…The polar pigmentation phenotype was also seen in omb insertion of white + -marked gene disruption elements such as P(XP) or piggyBacWH. 41 Several insertions from the screens of Peter et al (2002) 39 and Tang and Sun (2002), 40 reported to be independent, clustered to identical positions. Remarkably, the insertion omb P14 lies just 108 bp downstream of the upstream flanking gene CG3626 (which according to FlyAtlas is expressed in an unrelated pattern 42 ) but more than 40 kb upstream of the omb transcription start site (TSS).…”

Drosophila Mis12 Complex Acts as a Single Functional Unit Essential for Anaphase Chromosome Movement and a Robust Spindle Assembly Checkpoint

“…P{lacW}G0237 (Peter et al 2002), mis12 PBac{WH}f03756 (Thibault et al 2004), and the Df(3L)BSC27, Df(3L)BSC224 deficiencies were obtained from the Bloomington Stock Center. Stocks for Df(1)52 and Dp(1:Y)B s-v 1 y 1 (Meller and Rattner 2002) were kindly provided by Victoria Meller.…”

A Genetic Screen Identifies New Regulators of Steroid-Triggered Programmed Cell Death in Drosophila

“…P-element induced lethal mutations on the X and third chromosomes were obtained from the Bloomington Drosophila Stock Center. The X chromosome collection consisted of 398 stocks that included most lethal alleles generated in the original Pelement mutagenesis screen (Peter et al 2002). The third chromosome collection consisted of 467 lethal P-element insertions characterized and reduced to a unigene set by the Berkeley Drosophila Genome Project (Bellen et al 2004).…”