Manufacture of Fermentable Sugar Solutions from Sugar Cane Bagasse Hydrolyzed with Phosphoric Acid at Atmospheric Pressure

Abstract

Sugar cane bagasse, a renewable and cheap bioresource, was hydrolyzed at 100 degrees C using phosphoric acid at different concentrations (2, 4, or 6%) and reaction times (0-300 min) to obtain fermentable sugar solutions, which have a high concentration of sugars (carbon source for microorganism growth) and a low concentration of growth inhibitors (acetic acid and furfural). Xylose, glucose, arabinose, acetic acid, and furfural were determined following the hydrolysis. Kinetic parameters of mathematical models for predicting these compounds in the hydrolysates were obtained. Derived parameters such as efficiency of hydrolysis or purity of hydrolysates were considered to select as optimal conditions 6% phosphoric acid at 100 degrees C for 300 min. Using these conditions, 21.4 g of sugars L(-)(1) and <4 g of inhibitors L(-)(1) were obtained from the hydrolysis with a water/solid ratio of 8 g of water g(-)(1) of sugar cane bagasse on a dry basis.

Sugar cane bagasse prehydrolysis using hot water

“…The results in Table 2 shows that a xylose extraction yield (24.5-42.5 g/L) between 46 and 81% was achieved. Other authors (Gámez et al, 2004(Gámez et al, , 2006, using 4% H 3 PO 4 and 300 min at 122°C in the prehydrolysis of sugar cane bagasse reported obtaining 17.6 g of xylose/L; 2.6 g of arabinose/L; 3.0 g of glucose/L and 1.2 g furfural/L. Rodríguez-Chong et al (2004) performed the hydrolysis with 6% HNO 3 and 9.3 min at 122°C.…”

“…Several studies of sugar cane bagasse hydrolysis with H 3 PO 4 , HNO 3 and other dilute acids are presented (Gámez et al, 2004(Gámez et al, , 2006Rodríguez-Chong et al, 2004;Lavarack et al, 2002;Martín, 2002;Laopaiboon et al, 2010 ;Canilha et al, 2008).…”

Kinetic Modelling of Dilute Acid Hydrolysis of Lignocellulosic Biomass

“…The reaction rate of hydrolysis depends on a number of variables, such as: temperature, acid concentration, time, substrate concentration and substrate composition. The practical objective of studying the kinetic model is, on a first level, to optimize the process and, on a second level, to obtain Equations useful for economical estimations 4 . The models usually associated with dilute acid hydrolysis were first proposed by Saeman 7 , for the hydrolysis of Douglas fir wood using www.intechopen.com Kinetic Modelling of Dilute Acid Hydrolysis of Lignocellulosic Biomass 295 sulphuric acid.…”

“…The use of both models has been demonstrated by Gámez et al 4 in their efforts to hydrolyze sugar cane bagasse into fermentable sugars. The hemicelluloses of sugar cane are primarily xylan.…”

Hidrólise de polpa de sisal como via de produção de etanol e materiais

“…Neste caso, diversos estudos foram realizados envolvendo diferentes tipos de ácidos como H2SO4, HCl, H3PO4, ácido fórmico entre outros (LENIHAN et al, 2010;TIAN et al, 2010;SUN et al, 2008;GÁMEZ et al, 2004; SIVERS e ZACCHI, 1995 Com relação à celulose, os fatores importantes são: (1) se a reação ocorre em fase homogênea, ou em fase heterogênea, e (2) a acessibilidade, no caso de hidrólise heterogênea (GURGEL, 2010).…”