Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology

Munro, Barbara Hazard

doi:10.1016/s0031-3025(16)39410-7

Cited by 239 publications

(271 citation statements)

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“…Following fixation, tissue samples were processed according to Luna (1968) as it is the best method for treating small biopsies of endocrine organs. 8,9 Then, samples were labelled and embedded in paraffin as blocks. Paraffin blocks were sectioned into 4-6 μm thick slices using a disposable blade for each block.…”

Section: Methodsmentioning

confidence: 99%

The significance of hypoxia as a molecular and cellular event in patients with toxic and non-toxic goitre: A statistical inference based on cross-sectional analytic of Iraqi patients

Assi

Elewi

Al-Imam³

et al. 2018

Asian J Med Sci

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Background: Disorders of the thyroid gland, including toxic and non-toxic goitre, are more common in adult female patients. Hypoxia-inducible factors, including HIF-1 and HIF-2, represent transcriptional activators that function as regulators of oxygen homeostasis. Rapid progress is being made in clarifying the homeostatic functions of HIFs in several physiological systems. However, there are much to be learned in connection with the thyroid gland and its pathologies.Aims and Objectives: To assess the biochemical and clinical significance of hypoxia-inducible factors in patients with multinodular goitre.Materials and Methods: The study is observational and cross-sectional analytic conducted among a population of individuals with diffuse and nodular thyroid goitre including clinically toxic and non-toxic patients. It will attempt to answer the research question concerning the significance of HIFs, via applying techniques of immunohistochemistry to histological samples of resected thyroid tissue, and in pertinence with the demographic and clinical parameters of patients.Results: The total number of patients was forty-three,and most was in their fifth decades of life. The percentile contribution of males and females was 11.63% and 88.37% respectively. Individuals with toxic goitres accounted for 13.95%, and those had significantly higher levels of both HIF-1 and HIF-2 than non-toxic patients (p=0.019, p=0.072). Clinically-toxic patients also had notably more elevated levels of HIF-1 as they grew older when compared to non-toxic patients.Conclusion: There is an evident inadequacy of published literature on hypoxia-inducible factors in patients with goitre. There are no studies whatsoever in correspondence to the research questions explored in this study. Future attempts should explore experimental designs while using human as well as animal models and from an interdisciplinary perspective.Asian Journal of Medical Sciences Vol.9(5) 2018 44-49

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Section: Methodsmentioning

confidence: 99%

The significance of hypoxia as a molecular and cellular event in patients with toxic and non-toxic goitre: A statistical inference based on cross-sectional analytic of Iraqi patients

Assi

Elewi

Al-Imam³

et al. 2018

Asian J Med Sci

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“…The tissues were processed, embedded in paraffin, and sectioned at 6 lm. Tissue sections were stained with either hematoxylin and eosin (H&E) or Masson's trichrome stain using standard protocols or immunostained using standard protocols (Carson and Hladik 2009;Munro 1971). KRT14 immunostaining was performed using the standard avidin-biotin-peroxidase technique previously reported .…”

Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

Uterine Patterning, Endometrial Gland Development, and Implantation Failure in Mice Exposed Neonatally to Genistein

Jefferson

Padilla-Banks

Suen

et al. 2020

Environ Health Perspect

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BACKGROUND: Embryo implantation relies on precise hormonal regulation, associated gene expression changes, and appropriate female reproductive tract tissue architecture. Female mice exposed neonatally to the phytoestrogen genistein (GEN) at doses similar to those in infants consuming soybased infant formulas are infertile due in part to uterine implantation defects. OBJECTIVES: Our goal was to determine the mechanisms by which neonatal GEN exposure causes implantation defects. METHODS: Female mice were exposed to GEN on postnatal days (PND)1-5 and uterine tissues collected on PND5, PND22-26, and during pregnancy. Analysis of tissue weights, morphology, and gene expression was performed using standard histology, confocal imaging with threedimensional analysis, real-time reverse transcription polymerase chain reaction (real-time RT-PCR), and microarrays. The response of ovariectomized adults to 17b-estradiol (E2) and artificial decidualization were measured. Leukemia inhibitory factor (LIF) injections were given intraperitoneally and implantation sites visualized. Gene expression patterns were compared with curated data sets to identify upstream regulators. RESULTS: GEN-exposed mice exhibited reduced uterine weight gain in response to E2 treatment or artificial decidualization compared with controls; however, expression of select hormone responsive genes remained similar between the two groups. Uteri from pregnant GEN-exposed mice were posteriorized and had reduced glandular epithelium. Implantation failure was not rescued by LIF administration. Microarray analysis of GEN-exposed uteri during early pregnancy revealed significant overlap with several conditional uterine knockout mouse models, including Foxa2, Wnt4, and Sox17. These models exhibit reduced endometrial glands, features of posteriorization and implantation failure. Expression of Foxa2, Wnt4, and Sox17, as well as genes important for neonatal uterine differentiation (Wnt7a, Hoxa10, and Msx2), were severely disrupted on PND5 in GEN-exposed mice. DISCUSSION: Our findings suggest that neonatal GEN exposure in mice disrupts expression of genes important for uterine development, causing posteriorization and diminished gland function during pregnancy that contribute to implantation failure. These findings could have implications for women who consumed soy-based formulas as infants. https://doi.

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“…Necropsy was performed and gross pathological changes were recorded and photographed. The lungs samples were preserved in 10% neutral buffered formalin, then trimming, dehydration, clearing, paraffine embedding, sectioning, hematoxylin and eosin staining, and DPX mounting were performed (Luna, 1968). Lung sections were examined under light microscope (OPTIKA, Italy), photographed by a digital camera (Samsung/ South Korea) and the histopathological changes were recorded.…”

Section: Experimental Designmentioning

confidence: 99%

Detection of Lung Affections of Stray Cats in Mosul City, Iraq.

Al-Mallah¹,

Saeed²

2019

jwpr

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Stray cats are exposed to deleterious factors in the urban environment. The present study was aimed to describe the pathological features of lung lesions in stray cats in Mosul city, Iraq. From February to March 2013, 19 ailing cats were caught through animal control campaigns and euthanized. Necropsy and histopathologic findings were recorded for the collected lungs. The results indicated lesions in all the lung samples. Pathomorphogical characterization included emphysema (84%), atelectasis (63%), and bronchiectasis (26%), bronchopneumonia (63%), granulomatous pneumonia (15%), verminous pneumonia (15%), alveolitis (15%), proliferative pneumonia (10%), and pleuropneumonia (5%). In addition, cellular adaptation was characterized by hyperplasia of alveolar cells (52%), bronchial epithelium hyperplasia (31%) and fibroplasia (26%). Hemosiderosis and parasitic infestation were also detected. The study concluded that all lungs collected from stray cats showed pathological changes, reflecting the presence of the pathogen agents and pollution in the environment of this city.

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Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology

Cited by 239 publications

References 0 publications

The significance of hypoxia as a molecular and cellular event in patients with toxic and non-toxic goitre: A statistical inference based on cross-sectional analytic of Iraqi patients

The significance of hypoxia as a molecular and cellular event in patients with toxic and non-toxic goitre: A statistical inference based on cross-sectional analytic of Iraqi patients

Uterine Patterning, Endometrial Gland Development, and Implantation Failure in Mice Exposed Neonatally to Genistein

Detection of Lung Affections of Stray Cats in Mosul City, Iraq.

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