Mannose-sensitive interaction of Escherichia coli with human peripheral leukocytes in vitro

Abstract

The ability of Escherichia coli which possess or lack mannose-sensitive adherence factors (adhesins) to associate with human peripheral leukocytes in vitro in the absence of serum was studied. E. coli 19+, which have mannose-sensitive adhesins, were derived from E. coli strain 19 by culturing in static Trypticase soy broth at 37 degrees C. E. coli 19-, which lack mannose-sensitive adhesins, were derived from E. coli 19 by culturing in agitated Trypticase soy broth at 30 degrees C. E. coli 19+ attached to leukocytes and stimulated the release of lysozyme but not beta-glucuronidase or lactate dehydrogenase. In contrast, E. coli 19- showed poor attachment to the leukocytes and failed to stimulate lysosomal enzyme release. During a 60-min incubation with the leukocytes, the number of viable 19+ organisms decreased, whereas the number of viable 19- remained constant. Purified type 1 pili from E. coli 19+ agglutinated the leukocytes but did not stimulate lysosomal enzyme release. Pretreatment of leukocytes with type 1 pili failed to prevent the adherence of E. coli 19+. The association of 19+ with leukocytes and subsequent release of lysozyme could be blocked by alpha-methyl-D-mannoside but not by equivalent concentrations of dextrose and sucrose. These results show that mannose-sensitive adhesins on E. coli mediate association of the organisms with leukocytes in the absence of serum components. The identity of the adhesins involved in leukocyte association has yet to be determined.

Neutrophil chemiluminescence in response to Fusobacterium nucleatum

“…The monosaccharide D-galactose, which is a significant component of erythrocyte glycoproteins (Emerson & Kornfeid 1976) and F. nucleatum lipopolysaccharide (Fredriksen & Hofstad 1978, Kristoffersen & Hofstad 1970, caused significant inhibition of PMN chemiluminescence in response to F. nucleatum. Inhibition of PMN chemiluminescence required a moderately large concentration of Dgalactose (100 mM) compared to alpha-methyl-D-mannoside inhibition of PMN chemiluminescence in response to Fscherichia fo/j (Mangan & Snyder 1979). The inhibition of chemiluminescenee by D-gaiactose appeared to be a reversible, non-covalent interaction since centrifugation washes were able to restore a normal PMN response to F. nucleatum.…”

A non‐lectin‐like mechanism by which Fusobacterium nucleatum 10953 adheres to and activates human lymphocytes

“…No fimbriae, pili, or flagella were seen on either strain when negatively stained and examined by transmission electron microscopy (not shown). In comparison, pili on Escherichia coti were readily visible using this procedure (11).…”

Opsonin-independent ligation of Fc gamma receptors. The 3G8-bearing receptors on neutrophils mediate the phagocytosis of concanavalin A-treated erythrocytes and nonopsonized Escherichia coli.

“…These growth conditions yield smooth E. coli that possess mannose-sensitive adhesions . Mannose binding capacity, shown by other investigators to be related to the presence oftype I pili, was demonstrated by the capacity of this E. coli isolate to agglutinate guinea pig erythrocytes (24,25) and saccharomyces cerevisiae yeast cells (10) as determined by the methods of Eden and Hansson (24) and Bar Shavit et al (10), respectively . This agglutination was specifically inhibited by 0.20 M a-methylmannoside .…”