Manganese enhances the expression of the manganese superoxide dismutase in cultured primary chick embryonic myocardial cells

Abstract

In the present study, the effect of manganese (Mn) on antioxidant status and the expression of the manganese superoxide dismutase (MnSOD) gene in cultured primary myocardial cells collected from the chick embryos was investigated. The hypothesis that Mn supplementation would enhance the expression of MnSOD in cultured primary myocardial cells of chick embryos was tested. Eggs collected from Mn-depleted Arbor Acres laying breeder hens were incubated for 10 days and then myocardial cells were isolated and cultivated for 8 days. The embryonic myocardial cells on day 6 were treated with Mn in the cell culture medium at different time points when the proportion of cells showing spontaneous contraction was over 95% after the 3-day primary culture. A completely randomized design involving a 3 Mn levels (0, 0.5 and 1.0 mmol L-1)×3 incubation time points (12, 24 and 48 h) factorial arrangement of treatments (n=6) was used in the current experiment. The results showed that MnSOD activity and mRNA expression level were induced by Mn and increased with incubation time, which supported the hypothesis that Mn would enhance the expression of the MnSOD gene, and thus might protect myocardial cells from oxidative stress during the chick embryonic development.

Effects of Manganese Hydroxychloride on Growth Performance, Antioxidant Capacity, Tibia Parameters and Manganese Deposition of Broilers

“…As a component of MnSOD, Mn can improve broiler’s antioxidant ability by catalyzing the reduction of superoxide anion to hydrogen peroxide [ 35 ]. An in vitro study indicated that MnSOD activity and mRNA expression level in chick embryonic myocardial cells were improved by 1.0 mmol/L of Mn as MnCl 2 treatment [ 36 ]. Studies also have shown that dietary inclusion of Mn as MnSO 4 , Mn methionine, or Mn oxide improved MnSOD, CAT, and GSH-Px activities and T-AOC, while reducing MDA level in serum, liver, and leg muscle of broilers [ 25 , 37 ].…”

Metabolic utilization of intravenously injected iron from different iron sources in target tissues of broiler chickens

“…Then they were centrifuged for 4 min (12,000 × g , 4 °C), and the supernatants were subjected to western-blot analysis ( Zhang et al., 2017 ). The GAPDH protein was used to normalize the expression levels of SDH, CAT, COX or FTH protein ( Qin et al., 2017 ).…”

Organic iron absorption and expression of related transporters in the small intestine of broilers

“…The primary antibodies and dilution rates were as follows: DMT1, bs-3577R, 1:500 (Bioss, Beijing, China); FPN1, bs-4906R, 1:500 (Bioss); and GAPDH, ab22555, 1:5,000 (Abcam, Cambridge, MA). The GAPDH protein was used to normalize the expression levels of the target protein ( Qin et al., 2017 ).…”