Manantiales salinos: Inventarios de Diversidad Metabólica y filogenética de microorganismos de ambientes salinos

Díaz‐Cárdenas, Carolina; Baena, Sandra

doi:10.18257/raccefyn.199

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…The strain with the highest levels of cytotoxic activity was Labrenzia aggregata USBA 371 which was isolated from the Salado de Consotá salt spring (Díaz-Cárdenas and Baena 2015 ). Organisms of the Labrenzia genus isolated from marine sponges have presented antimicrobial activity against B. subtilis and MRSA (Graça et al 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…Both halophilic microorganisms isolated from Zipaquirá salt mine and other previously isolated strains were used for screening in this study. These previously isolated strains were obtained in a previous study from two saline springs in the Central Mountain Range of the Colombian Andes and are part of our collection of microorganisms (Díaz-Cárdenas and Baena 2015 ): Oceanibaculum indicum USBA 36, Caenispirillum bisanense USBA 85, Shewanella chilikensis USBA 344 and Labrenzia aggregata USBA 371. All of the strains evaluated for the cytotoxic screenings were deposited in the Colección de microorganismos de la Pontificia Universidad Javeriana (WDCM857).…”

Section: Methodsmentioning

confidence: 99%

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Microbial diversity of saline environments: searching for cytotoxic activities

et al. 2017

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In order to select halophilic microorganisms as a source of compounds with cytotoxic activities, a total of 135 bacterial strains were isolated from water and sediment samples collected from the Zipaquirá salt mine in the Colombian Andes. We determined the cytotoxic effects of 100 crude extracts from 54 selected organisms on the adherent murine mammary cell carcinoma 4T1 and human mammary adenocarcinoma MCF-7 cell lines. These extracts were obtained from strains of Isoptericola, Ornithinimicrobium, Janibacter, Nesterenkonia, Alkalibacterium, Bacillus, Halomonas, Chromohalobacter, Shewanella, Salipiger, Martellela, Oceanibaculum, Caenispirillum and Labrenzia. The extracts of 23 strains showed an IC50 of less than 100 μg mL−1. They were subsequently analyzed by LC/MS allowing dereplication of 20 compounds. The cytotoxic effect was related to a complex mixture of diketopiperazines present in many of the extracts analyzed. The greatest cytotoxic activity against both of the evaluated cell lines was obtained from the chloroform extract of Labrenzia aggregata USBA 371 which had an IC50 < 6 μg mL−1. Other extracts with high levels of cytotoxic activity were obtained from Bacillus sp. (IC50 < 50 μg mL−1) which contained several compounds such as macrolactin L and A, 7-O-succinoylmacrolactin F and iturin. Shewanella chilikensis USBA 344 also showed high levels of cytotoxic activity against both cell lines in the crude extract: an IC50 < 15 μg mL−1 against the 4T1 cell line and an IC50 < 68 μg mL−1 against the MCF-7 cell line. Nesterenkonia sandarakina CG 35, which has an IC50 of 118 µg mL−1 against 4T1, is a producer of diketopiperazines and 1-acetyl-β-carboline. Also, Ornithinimicrobium kibberense CG 24, which has IC50 < 50 μg mL−1, was a producer of diketopiperazines and lagunamycin. Our study demonstrates that these saline environments are habitats of halophilic and halotolerant bacteria that have previously unreported cytotoxic activity.Electronic supplementary materialThe online version of this article (10.1186/s13568-017-0527-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Microbial diversity of saline environments: searching for cytotoxic activities

et al. 2017

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Subsurface Stappia: Success Through Defence, Specialisation and Putative Pressure-Dependent Carbon Fixation

et al. 2019

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Draft genome and description of Consotaella salsifontis gen. nov. sp. nov., a halophilic, free-living, nitrogen-fixing alphaproteobacterium isolated from an ancient terrestrial saline spring

Díaz‐Cárdenas

Bernal

Caro‐Quintero

et al. 2017

International Journal of Systematic and Evolutionary Microbiology

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A free-living, nitrogen-fixing, mesophilic and facultative aerobe, designated strain USBA 369, was isolated from a terrestrial saline spring of the Colombian Andes. The non-sporulating rods (1.5×0.8 µm) with rounded ends stained Gram-negative and were motile by means of lophotrichous flagella. The strain grew optimally at 30 °C, at pH 6.9-7.5 and with 1.5 % (w/v) NaCl. The major fatty acids detected were C18 : 1ω7c and C19 : 0 cyclo ω8c, and the respiratory lipoquinone ubiquinone 10 (Q-10) was present. The genome consisted of 4.65 Mb with a DNA G+C content of 64.3 mol%. A total of 4371 genes were predicted and, of those, 4300 were protein coding genes and 71 were RNA genes. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain USBA 369 formed a different lineage within the class Alphaproteobacteria, order Rhizobiales, and DNA homology studies with the most closely related genera, Aurantimonas, Aureimonas and Rhizobium (95 % 16S rRNA gene sequence similarity), showed values of <15 %. The phylogenomic analysis provided evidence for clear phylogenetic divergence between strain USBA 369 and the closely related genera. On the basis of the phenotypic, chemotaxonomic and phylogenomic evidence, strain USBA 369 is considered to represent a novel genus and a novel species for which the name Consotaella salsifontis gen. nov., sp. nov. is proposed. The type strain is USBA 369 (=KCTC 22549=CMPUJ U369).

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Manantiales salinos: Inventarios de Diversidad Metabólica y filogenética de microorganismos de ambientes salinos

Cited by 3 publications

References 53 publications

Microbial diversity of saline environments: searching for cytotoxic activities

Microbial diversity of saline environments: searching for cytotoxic activities

Subsurface Stappia: Success Through Defence, Specialisation and Putative Pressure-Dependent Carbon Fixation

Draft genome and description of Consotaella salsifontis gen. nov. sp. nov., a halophilic, free-living, nitrogen-fixing alphaproteobacterium isolated from an ancient terrestrial saline spring

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