Malvidin-3-glucoside protects endothelial cells up-regulating endothelial NO synthase and inhibiting peroxynitrite-induced NF-kB activation

Paixão, Joana; Dinis, Teresa C.P.; Almeida, Leonor M.

doi:10.1016/j.cbi.2012.08.013

Cited by 72 publications

(46 citation statements)

References 45 publications

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“…In the last years, many studies have been carried out demonstrating the important role of anthocyanins in the prevention and treatment of chronic inflammatory diseases [5,7].…”

Section: Discussionmentioning

confidence: 99%

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Cyanidin-3-glucoside suppresses cytokine-induced inflammatory response in human intestinal cells: comparison with 5-aminosalicylic acid

Serra¹,

Paixão²,

Nunes³

et al. 2012

Free Radical Biology and Medicine

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The potential use of polyphenols in the prevention and treatment of chronic inflammatory diseases has been extensively investigated although the mechanisms involved in cellular signaling need to be further elucidated. Cyanidin-3-glucoside is a typical anthocyanin of many pigmented fruits and vegetables widespread in the human diet. In the present study, the protection afforded by cyanidin-3-glucoside against cytokine-triggered inflammatory response was evaluated in the human intestinal HT-29 cell line, in comparison with 5-aminosalicylic acid, a well-established anti-inflammatory drug, used in inflammatory bowel disease. For this purpose, some key inflammatory mediators and inflammatory enzymes were examined. Our data showed that cyanidin-3-glucoside reduced cytokine-induced inflammation in intestinal cells, in terms of NO, PGE 2 and IL-8 production and of iNOS and COX-2 expressions, at a much lower concentration than 5-aminosalicylic acid, suggesting a higher anti-inflammatory efficiency. Interestingly, cyanidin-3-glucoside and 5-aminosalicylic acid neither prevented IkB-a degradation nor the activation of NF-kB, but significantly reduced cytokine-induced levels of activated STAT1 accumulated in the cell nucleus. In addition, we established that phosphorylated p38 MAPK was not involved in the protective effect of cyanidin-3-glucoside or 5-aminosalicylic acid. Taking into account the high concentrations of dietary anthocyanins potentially reached in the gastrointestinal tract, cyanidin-3-glucoside may be envisaged as a promising nutraceutical giving complementary benefits in the context of inflammatory bowel disease.

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“…In the last years, many studies have been carried out demonstrating the important role of anthocyanins in the prevention and treatment of chronic inflammatory diseases [5,7].…”

Section: Discussionmentioning

confidence: 99%

“…Due to their relatively high consumption, the impact of anthocyanins on health promotion and disease prevention has been extensively investigated in the last decades [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Cyanidin-3-glucoside suppresses cytokine-induced inflammatory response in human intestinal cells: comparison with 5-aminosalicylic acid

Serra¹,

Paixão²,

Nunes³

et al. 2012

Free Radical Biology and Medicine

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show abstract

“…Bittar et al [105] showed that anthocyanin analogues had a catechol group, which is associated to metal-binding and antioxidant properties. A study reported that the anthocyanin, eggplant peels the delphinidin-3-( p -coumaroylrutinoside)-5-glucoside (nasunin), has antioxidant potent activity by chelating ferrous-dependent mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…Thereby, anthocyanins also act inhibiting NF-κB and NF-κB -dependent mediators. Paixão et al [105] showed that malvidin-3-glucoside could suppress pro-inflammatory mediators through of the NF-κB inhibition in bovine arterial endothelial cell. Another research showed that malvidin inhibited the TNF-α, and it blocked the MCP-1, ICAM-1 and VCAM-1 expression induced by TNF-α [122].…”

Section: Introductionmentioning

confidence: 99%

Action mechanism and cardiovascular effect of anthocyanins: a systematic review of animal and human studies

et al. 2016

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Cardiovascular diseases (CVD) are an important cause of death worldwide. Anthocyanins are a subgroup of flavonoids found in berries, flowers, fruits and leaves. In epidemiological and clinical studies, these polyphenols have been associated with improved cardiovascular risk profiles as well as decreased comorbidities. Human intervention studies using berries, vegetables, parts of plants and cereals (either fresh or as juice) or purified anthocyanin-rich extracts have demonstrated significant improvements in low density lipoproteins oxidation, lipid peroxidation, total plasma antioxidant capacity, and dyslipidemia as well as reduced levels of CVD molecular biomarkers. This review discusses the use of anthocyanins in animal models and their applications in human medicine, as dietary supplements or as new potent drugs against cardiovascular disease.

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“…Java plum (Syzygium cumini) restored the body weight, glucose, urea and creatinine levels of diabetic rats to normal levels. Amazon fruits, such as açaí and pequiá (Caryocar villosum) exerted in vivo antigenotoxic and antimutagenic effects (ALMEIDA et al, 2012;RIBEIRO et al, 2010).…”

Section: Fruit and Vegetable Intake And Guajiru (Chrysobalanus Icaco L)mentioning

confidence: 99%

Atividades in vitro e in vivo do fruto do guajiruzeiro (Chrysobalanus icaco L.) em biomarcadores de estresse oxidativo, danos ao DNA e inflamação e inflamação

Venâncio¹

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Guajiru (Chrysobalanus icaco L.) is a fruit rich in anthocyanins, which exert several beneficial effects on health. Although guajiru leaves are used in folk medicine as hypoglycemic and antioxidant, the fruit effects on health remain unknown. The aim of this study was to evaluate the effects of guajiru fruit against in vivo DNA damage and oxidative stress and in vivo/in vitro inflammation. Male Wistar rats (4-5 weeks old, 110 g) were divided into eight groups and treated for 14 days with water or lyophilized guajiru fruit (100, 200 or 400 mg/kg b.w.) by gavage. On the 14 th day, animals received physiologic solution or DXR (15 mg/kg b.w. i.p.) and were euthanized after 24 hours. Genotoxicity and antigenotoxicity were evaluated by comet assay in peripheral blood, liver, kidney, and heart. Mutagenicity and antimutagenicity of guajiru fruit were investigated by micronucleus test in peripheral blood and bone marrow. The oxidative burst was measured in peripheral blood neutrophils. Oxidative stress parameters involved the concentration of thiobarbituric acid reactive substances, reduced/oxidized glutathione ratio, and catalase activity in liver, kidney and heart. The expressions of DNA damage/repair genes Gadd45a (growth arrest and DNA damage-inducible alpha), Parp1 (Poly(ADP-ribose) polymerase 1), and Xrcc2 (X-Ray Repair complementing defective repair in Chinese hamster cells 2) and pro-inflammatory markers Il-1β (interleukin 1 beta), Il-6 (interleukin 6), Nf-κb (nuclear factor kappa B), and Tnf-α (tumor necrosis factor alpha) were evaluated by real-time quantitative PCR. Human colon cell lines CCD18Co (fibroblasts), and HT-29 (adenocarcinoma) were treated with guajiru anthocyanins (1.0 -20.0 mg/L gallic acid equivalents -GAE) and the expressions of IL-1β, IL-6, NF-κB and TNF-α were analyzed at mRNA and protein levels. TNF-α was used to induce inflammation in CCD-18Co cells. Guajiru fruit phytochemicals were quantified and characterized by chromatographic and spectrometric methods. The concentrations of 19 chemical elements were determined by inductively coupled plasma mass spectrometry (ICP-MS). Delphinidin, cyanidin, petunidin and peonidin were the major anthocyanins in this fruit. Significant amounts of phytochemicals, magnesium, and selenium were found in this fruit. Guajiru fruit displayed in vivo antioxidant activity in neutrophils, antigenotoxicity in peripheral blood and antimutagenicity in bone marrow and peripheral blood. Guajiru fruit decreased DNA damage in liver, kidney, and heart. This fruit decreased the expression of Gadd45a, Il-1β, and Tnf-α in tissues. Cell proliferation was suppressed in HT-29 cells, and this was accompanied by increased intracellular ROS production as well as decreased TNF-α, , and NF-κB expressions. There was no cytotoxic effect of guajiru fruit anthocyanins in CCD-18Co cells. IL-1β, IL-6, and TNF-α protein expressions were reduced in TNF-α-treated CCD-18Co cells by guajiru fruit anthocyanins. The findings from this investigation demonstrated that phytochemicals and chemical ele...

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Malvidin-3-glucoside protects endothelial cells up-regulating endothelial NO synthase and inhibiting peroxynitrite-induced NF-kB activation

Cited by 72 publications

References 45 publications

Cyanidin-3-glucoside suppresses cytokine-induced inflammatory response in human intestinal cells: comparison with 5-aminosalicylic acid

Cyanidin-3-glucoside suppresses cytokine-induced inflammatory response in human intestinal cells: comparison with 5-aminosalicylic acid

Action mechanism and cardiovascular effect of anthocyanins: a systematic review of animal and human studies

Atividades in vitro e in vivo do fruto do guajiruzeiro (Chrysobalanus icaco L.) em biomarcadores de estresse oxidativo, danos ao DNA e inflamação e inflamação

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