Malignancy and Tissue Metabolism

Shrivastava, Garima; Quastel, J. H.

doi:10.1038/196876a0

Cited by 22 publications

(8 citation statements)

References 13 publications

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“…This inference was further supported by a significant decrease in the 3 H-thymidine uptake as well as enzyme activity of LDH in combined phytochemicals supplemented benzo[a]pyrene group. The results are in sync with earlier reports [24]–[27].…”

Section: Discussionsupporting

confidence: 93%

Study to Evaluate Molecular Mechanics behind Synergistic Chemo-Preventive Effects of Curcumin and Resveratrol during Lung Carcinogenesis

2014

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BackgroundThe combination approach is the future of the war against cancer and the present study evaluated molecular mechanics behind the synergistic effects of curcumin and resveratrol during lung carcinogenesis.MethodsThe mice were segregated into five groups which included normal control, Benzo[a]pyrene[BP] treated, BP+curcumin treated, BP+resveratrol treated and BP+curcumin+resveratrol treated.ResultsThe morphological analyses of tumor nodules confirmed lung carcinogenesis in mice after 22 weeks of single intra-peritoneal[i.p] injection of BP at a dose of 100 mg/Kg body weight. The BP treatment resulted in a significant increase in the protein expressions of p53 in the BP treated mice. Also, a significant increase in the protein expression of phosphorylated p53[ser15] confirmed p53 hyper-phosphorylation in BP treated mice. On the other hand, enzyme activities of caspase 3 and caspase 9 were noticed to be significantly decreased following BP treatment. Further, radiorespirometric studies showed a significant increase in the 14C-glucose turnover as well as 14C-gulcose uptake in the lung slices of BP treated mice. Moreover, a significant rise in the cell proliferation was confirmed indirectly by enhanced uptake of 3H-thymidine in the lung slices of BP treated mice. Interestingly, combined treatment of curcumin and resveratrol to BP treated animals resulted in a significant decrease in p53 hyper-phosphorylation, 14C glucose uptakes/turnover and 3H-thymidine uptake in the BP treated mice. However, the enzyme activities of caspase 3 and caspase 9 showed a significant increase upon treatment with curcumin and resveratrol.ConclusionThe study, therefore, concludes that molecular mechanics behind chemo-preventive synergism involved modulation of p53 hyper-phosphorylation, regulation of caspases and cellular metabolism enzymes.

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Section: Discussionsupporting

confidence: 93%

Study to Evaluate Molecular Mechanics behind Synergistic Chemo-Preventive Effects of Curcumin and Resveratrol during Lung Carcinogenesis

2014

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“…The uptake rates of some amino acids (lysine, arginine, proline, glutamine, glycine, alanine, cystine, methionine and ammonia) were negative in normal tissue, and the serum concentration of asparagic acid could not be detected in more than 10 patients. 1 Refers to essential amino acids, 2 refers to non-essential amino acids, 3 refers to ketogenic and glucogenic amino acids, 4 refers to ketogenic amino acids. Relationships between the uptake rates of amino acids and the size of tumor mass or the Dukes stage are shown in Table 2 and Table 3.…”

Section: Resultsmentioning

confidence: 99%

“…Studies note the significance of amino acid metabolism in neoplasms, though there are lots of questions that remain to be answered [1][2][3][4][5] . It is helpful to improve the life quality if we take patients' nutrition into account according to their characteristic amino acid metabolism before we treat cancer patients.…”

Section: Introductionmentioning

confidence: 99%

Amino acid uptake in arterio-venous serum of normal and cancerous colon tissues

Wang¹,

Shen²,

Zhang³

et al. 2004

WJG

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“…The above mentioned increase in the 3 H-thymindine signifies increased DNA synthesis during rapid cell proliferation [41][42][43], which might be due to high activity of COX-2 and would have resulted in the slowdown of apoptotic machinery in BP treated mice. Also, increase in cell proliferations requires high amount of glucose to survive [44]. So, the observed increase in 14 Cglucose uptake is a result of increase in requirement of glucose by rapidly proliferating cells following BP treatment.…”

Section: Discussionmentioning

confidence: 98%

COX-2 as a Potential Target in Chemoprevention of Benzo(a)pyrene Induced Lung Carcinogenesis in Mice-combined Role of Curcumin and Quercetin

Nair¹,

Malhotra²,

Dhawan

2012

Am. J. Biomed. Sci.

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The present study was undertaken to assess the cumulative effects of curcumin and quercetin in inhibiting the activity of COX-2 alongwith other biophysical parameters such as 3 H thymidine uptake as well as uptake and turnover of 14 C-glucose during BP induced lung carcinogenesis in mice. The mice were segregated into five groups viz., normal control, BP treated, BP + curcumin treated, BP + quercetin treated and BP + curcumin+ quercetin treated. Lung carcinogenesis was induced by a single intra-peritoneal (IP) injection of BP (100mg/Kg body weight). Curcumin and quercetin were supplemented to mice at dose levels of 60mg/Kg body weight and 40mg/Kg body wt in drinking water, respectively. All the treatments were continued for a period of 22 weeks. BP treatment brought about a significant increase in the activities of COX-2. Supplementation of phytochemicals individually as well in combination to BP treated mice brought about moderation in the enzymatic activity which was restored to within normal limits. Further, BP treatment recorded increased 3 H thymidine uptake as well as enhanced 14 C-glucose uptake and its turnover which were reduced significantly following simultaneous treatment with phytochemicals. Therefore, cumulative treatment with curcumin and quercetin has the potential of providing protection against lung cancer by inhibiting the COX-2 activity and moderating the 3 H thymidine uptake as well as 14 C-glucose uptake and its turnover.

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Malignancy and Tissue Metabolism

Cited by 22 publications

References 13 publications

Study to Evaluate Molecular Mechanics behind Synergistic Chemo-Preventive Effects of Curcumin and Resveratrol during Lung Carcinogenesis

Study to Evaluate Molecular Mechanics behind Synergistic Chemo-Preventive Effects of Curcumin and Resveratrol during Lung Carcinogenesis

Amino acid uptake in arterio-venous serum of normal and cancerous colon tissues

COX-2 as a Potential Target in Chemoprevention of Benzo(a)pyrene Induced Lung Carcinogenesis in Mice-combined Role of Curcumin and Quercetin

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