Major seminal plasma proteome of rabbits and associations with sperm quality

Bezerra, Maria Júlia Barbosa; Arruda-Alencar, J; Martins, Jorge André Matias; Viana, Arabela Guedes de Azevedo; Neto, Aderson Martins Viana; Rêgo, João Paulo Arcelino do; Oliveira, Rodrigo Vasconcelos de; Lobo, Marina Duarte Pinto; Moreira, Ariele C.; Moreira, Renato A.; Moura, Arlindo A.

doi:10.1016/j.theriogenology.2019.01.013

Cited by 23 publications

(19 citation statements)

References 77 publications

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“…It would be possible to improve the sperm freezability and resolve the individual or breed differences by complemental supplementations according to the seminal plasma trait. In the rabbit, it was revealed that genotype, i.e., breed, affects the abundance of some seminal plasma proteins [ 103 ], which are associated with sperm quality [ 104 ]. Furthermore, there is no information about the association between sperm freezability and seminal plasma traits in rabbits, and further studies are needed.…”

Section: Discussionmentioning

confidence: 99%

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Nishijima

Kitajima

Matsuhisa

et al. 2021

Animals

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The rabbit is a valuable animal for both the economy and biomedical sciences. Sperm cryopreservation is one of the most efficient ways to preserve rabbit strains because it is easy to collect ejaculate repeatedly from a single male and inseminate artificially into multiple females. During the cooling, freezing and thawing process of sperms, the plasma membrane, cytoplasm and genome structures could be damaged by osmotic stress, cold shock, intracellular ice crystal formation, and excessive production of reactive oxygen species. In this review, we will discuss the progress made during the past years regarding efforts to minimize the cell damage in rabbit sperms, including freezing extender, cryoprotectants, supplements, and procedures.

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Section: Discussionmentioning

confidence: 99%

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Nishijima

Kitajima

Matsuhisa

et al. 2021

Animals

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“…In addition to the bovine, BSPs and their isoforms are present in the seminal plasma of other ruminants such as buffalos 34 , rams 35 , 36 and goats 37 . Horses 38 , rabbits 39 and humans 12 also express BSPs in semen, but in lower amounts than ruminants. Most studies about BSPs have been conducted in bulls and it is believed that BSPs interact with sperm at the moment of ejaculation, participate in capacitation 12 , mediate the interaction between spermatozoa and oviduct epithelia 40 and affect fertilization as well 19 .…”

Section: Discussionmentioning

confidence: 99%

Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability

Gomes

Park

Viana

et al. 2020

Sci Rep

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The present study investigated the seminal plasma proteome of Holstein bulls with low (LF; n = 6) and high (HF; n = 8) sperm freezability. The percentage of viable frozen-thawed sperm (%ViableSperm) determined by flow cytometry varied from-2.2 in LF to + 7.8 in HF bulls, as compared to the average %ViableSperm (54.7%) measured in an 860-sire population. Seminal proteins were analyzed by label free mass spectrometry, with the support of statistical and bioinformatics analyses. this approach identified 1,445 proteins, associated with protein folding, cell-cell adhesion, NADH dehydrogenase activity, ATP-binding, proteasome complex, among other processes. There were 338 seminal proteins differentially expressed (p < 0.05) in LF and HF bulls. Based on multivariate analysis, BSP5 and seminal ribonuclease defined the HF phenotype, while spermadhesin-1, gelsolin, tubulins, glyceraldehyde-3-phosphate dehydrogenase, calmodulin, ATP synthase, sperm equatorial segment protein 1, peroxiredoxin-5, secretoglobin family 1D and glucose-6-phosphate isomerase characterized the LF phenotype. Regression models indicated that %ViableSperm of bulls was related to seminal plasma peroxiredoxin-5, spermadhesin-1 and the spermadhesin-1 × BSP5 interaction (R 2 = 0.84 and 0.79; p < 0.05). This report is the largest dataset of bovine seminal plasma proteins. Specific proteins of the non-cellular microenvironment of semen are potential markers of sperm cryotolerance. Sperm cryopreservation, artificial insemination (AI) and in vitro fertilization followed by embryo transfer are among the most used assisted reproductive technologies (ARTs), allowing genetic selection of farm animals 1 , conservation of wild and endangered species 2 , successful pregnancies for infertile couples 3 and preservation of fertility in cancer patients 4. Successful implementation of ARTs depends on cost-effective and efficient cryopreservation of sperm cells. Cryopreservation protocols include the dilution of fresh semen with extender containing cryoprotectants, buffers and antibiotics, followed by freezing in liquid nitrogen. Freezing and thawing semen interferes with the structure of the sperm membranes, alters functions of membrane proteins and ion channels, causes premature capacitation and acrosome reaction and yields excessive reactive oxygen species 5. Additionally, cryopreservation reduces both sperm metabolism and mitochondrial activity and alters sperm chromatin structure 6. All these effects result in lower motility and fertilizing capacity of frozen-thawed sperm when compared to untreated cells. Despite substantial improvements of protocols for cryopreservation of sperm and development of new extenders, it is currently accepted that 40 to 50% of sperm are incapable of proper fertilization after freezing and thawing 1. Studies indicate that certain males with nearly identical sperm parameters measured in fresh ejaculates present contrasting sperm viability after cryopreservation 7 and some bulls with high reproductive performance in natural mating have poor ...

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“…The in vitro incubation of rabbit sperm with exogenous human β-NGF (100 ng/ml) maintained a high motility rate and track speed (17). Consistently, Bezerra et al (18) reported a positive association between the β-NGF protein expression in seminal plasma and rabbit sperm motility.…”

Section: Introductionmentioning

confidence: 60%

Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling

et al. 2021

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To provide new insights into the mechanisms through which seminal plasma proteins can protect sperm from damage caused during refrigeration, we evaluate the possibility that β-NGF can contribute to the improvement of sperm quality after cooling. First, β-NGF was detected in refrigerated sperm and compared with unrefrigerated sperm by western blotting of the proteins adsorbed by sperm, showing that native β-NGF is still present even 24 h after cooling only as an active form. Then, the effect of exogenous β-NGF on sperm quality after cooling was evaluated. A total of 12 ejaculates from male llamas (three ejaculates per male), were obtained by electro-ejaculation, diluted 4:1 with buffer Hepes-balanced salt solution and centrifuged at 800 × g for 8 min to remove the seminal plasma. Sperm were suspended in Tris-citrate-fructose-egg yolk diluent for a final concentration of 30 ×106/ml and cooled at 5°C for 24 h. After refrigeration, the extended sperm were equilibrated for 5 min at 37°C and divided into the following subgroups: sperm samples without treatment (control) and sperm samples supplemented with exogenous human β-NGF (10, 100, and 500 ng/ml). At 5, 30, and 60 min of incubation sperm were evaluated for sperm viability (using eosin/nigrosin stain), sperm motility and vigor (observed under light microscopy), and mitochondrial activity (using the JC-1 fluorescent marker). Vigor data were analyzed with the nonparametric Kruskal-Wallis test. The rest of the variables were analyzed with a mixed models approach. Mean comparisons were performed using Fisher's LSD test with a confidence level of 95%. A principal components analysis was performed to analyze the relationships between variables. Treatment of 24 h cooled sperm with 10 or 100 ng/ml of human β-NGF increased the percentage of total motility and vigor (p < 0.05). Besides, an incubation time of 60 min would be adequate to improve sperm quality, since all variables are positively related. The significant improvement observed in the motility and vigor of post-refrigerated sperm suggests that supplementation with exogenous β-NGF may be profitable for the improvement of cooled llama sperm.

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Major seminal plasma proteome of rabbits and associations with sperm quality

Cited by 23 publications

References 77 publications

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability

Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling

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