Main viruses in sweet cherry plantations of Central-Western Spain

Sánchez, Rodrigo Espinoza; Corts, Remedios Morales; Benavides, Pablo García; Sánchez, María Luisa Zagalaz

doi:10.1590/0103-9016-2014-0140

Cited by 6 publications

(7 citation statements)

References 22 publications

(30 reference statements)

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“…Around 56.2% of collected samples with serologically proved infection of PDV showed symptoms, whereas 43.8% were without symptoms. Our results are in agreement with Yardimci and Culal-Klllc [18], who observed PDV infection to be commonly associated with symptomatic cherry trees, whereas Perez-S anchez et al [19] reported 65%-100% asymptomatic reaction to the virus. Several types of symptoms ( Figure 1) were observed: A/chlorosis in the base of central and secondary veins; B/chlorotic spots and rings; C/mottling and wrinkling of the leaves; D/small necrotic spots; E/ chlorotic and necrotic dark-brown spots and stripes and F/reddish necrosis and rupture.…”

Section: Field Surveys and Symptoms Evaluationsupporting

confidence: 93%

“…This infection rate is similar to the infection rate (15.8%) of PDV in cherry in Bulgaria reported previously [22]. In contrast, another study found 62% PDV infection rate of sweet cherry in Spain [19]. Extended field studies in the countries of the Mediterranean region have revealed a high incidence of Ilarvirus infection (23.5%), with a prevalence of PDV (35.4%) in cherry [4].…”

Section: Serological Evaluationsupporting

confidence: 86%

“…Several types of symptoms ( Figure 1) were observed: A/chlorosis in the base of central and secondary veins; B/chlorotic spots and rings; C/mottling and wrinkling of the leaves; D/small necrotic spots; E/ chlorotic and necrotic dark-brown spots and stripes and F/reddish necrosis and rupture. Such symptoms induced by PDV have been already described [19][20][21]. It should also be noted that the symptoms described above were clearer at the beginning of the vegetation period (April, May and early June) and some of them were masked with the progression of the season.…”

Section: Field Surveys and Symptoms Evaluationsupporting

confidence: 61%

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Incidence and genetic diversity of Prune dwarf virus in sweet and sour cherry in Bulgaria

Kamenova

Borisova²,

Popov³

2019

Biotechnology & Biotechnological Equipment

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Sweet cherry is an important fruit crop in Bulgaria. One of the most common viruses of sweet cherry and other stone fruits in the world is Prune dwarf virus (PDV). Field surveys were conducted in 43 commercial and 3 collection sweet and sour cherry orchards at 10 locations in six regions of Bulgaria. Leaf samples from 2090 trees showing and not showing virus-like symptoms were tested by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) for PDV infection. The average PDV infection level in sweet and sour cherry was 14.4%. In 92.4% of the trees, PDV caused single infection, whereas in 7.6%, it caused mixed infection with Prunus necrotic ringspot virus (PNRSV). Visual observations were made to connect the symptoms with PDV infection. There were PDV symptoms in 56.2% of infected trees whereas, 43.8% of the trees were asymptomatic. The coat protein (CP) sequences of 14 isolates from four regions were determined. The obtained sequences were 654 nucleotides (nts) in length, translated to 218 amino acids. Sequence comparisons showed 87%-100% to 93%-100% identity at nucleotide and amino acid level, respectively. Phylogenetic analyses using nucleotide and amino acid sequences of the CP genes of PDV isolates from Bulgaria and 38 cherry isolates retrieved from the GenBank database indicated the existence of three and two phylogenetic groups, respectively. No association between the phylogenetic grouping of the studied isolates and the symptoms of infected trees and/or the sampling-region was observed.

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“…It should be noted that chlorosis was the most common symptom among all surveyed trees. These symptoms are characteristic of PDV infection and have been repeatedly described by a number of authors (Massart et al, 2008;Smith et al, 1988;Sanchez et al, 2015;Kamenova et al, 2019).…”

Section: Resultsmentioning

confidence: 70%

Variability of some isolates of Prunus necrotic ringspot virus and Prune dwarf virus infecting sour and sweet cherry in Ukraine

Pavliuk¹,

Udovychenko

Riaba³

et al. 2021

Acta Agric. Slov.

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Prunus necrotic ringspot virus (PNRSV) andPrune dwarf virus (PDV) are the most common pathogens in stone crop orchards. These diseases are easily transmitted with pollen and hence rapidly spread in orchards leading to stunting of trees, their increased susceptibility to abiotic stress factors and, eventually, to significant yield losses. In Ukraine, only monitoring studies on the spread of these viruses were conducted until now. However, phylogenetic comparison of Ukrainian isolates was lacking. In this work, total RNA was isolated from plant samples tested positive for PNRSV and PDV by ELISA. The part of viral CP gene sequences were amplified and sequenced with their subsequent phylogenetic analysis. It was determined that PNRSV isolates from Ukraine analyzed in this study belong to different groups -PV-96 (MT828889) and PV-32 (MT892676) with a maximum identity level of 100 % with known isolates from NCBI GenBank. PDV isolates (MT828888 and MT828887) showed high identity with each other (99.6 %), and Slovakian isolate from sweet cherry was shown as the most related to them with identity of 95.3 %.

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“…The virus is seldom accompanied by evident deformation of the leaf blades (Fulton 1980). Twenty one percent of PNRSV infected sweet cherry trees showing chlorotic ringspots that evolved to dark brown necrotic areas in both secondary veins and interveinal regions of the leaf were reported by Sanchez et al, (2004). Smith et al, (2009) also observed chlorotic and necrotic spots on the leaves of sweet cherry trees infected by PNRSV but the centres of these necrotic spots often disappeared, affording a shothole effect.…”

Section: Plate3 Pnrsv Induced Ringspots and Chlorotic Spots On Applementioning

confidence: 86%

Ilarviruses and the Importance of Certified Elite Planting Material in Apple Production System - An Overview

Kapoor¹,

Sharma²,

Shylla³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Main viruses in sweet cherry plantations of Central-Western Spain

Cited by 6 publications

References 22 publications

Incidence and genetic diversity of Prune dwarf virus in sweet and sour cherry in Bulgaria

Variability of some isolates of Prunus necrotic ringspot virus and Prune dwarf virus infecting sour and sweet cherry in Ukraine

Ilarviruses and the Importance of Certified Elite Planting Material in Apple Production System - An Overview

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