Magnoliae Flos Induces Apoptosis of RBL-2H3 Cells via Mitochondria and Caspase

Kim, Gyoo C.; Lee, Soon G.; Park, Bong S.; Kim, Jee Y.; Song, Yeon Suk; Kim, Jong Min; Yoo, Kisoo; Huh, Gi Y.; Jeong, Min Ho; Lim, Young Jin; Kim, Hyung M.; Yoo, Young Hyun

doi:10.1159/000070925

Cited by 21 publications

(15 citation statements)

References 19 publications

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“…The plants of the Magnoliaceae have been used in Korea as a medication for a variety of diseases, including allergic rhinitis, fever, headache, anxiety, diarrhea, stroke and asthma (Wang et al, 2000;Kim et al, 2003). Magnolia kobus is a medicinal plant of the Magnoliaceae and pharmacological activities of a flower bud of Magnolia kobus are well characterized.…”

Section: Discussionmentioning

confidence: 99%

“…Traditional Oriental medicine Magnoliae Flos, a dried flower bud of Magnolia kobus, Magnolia denudata or Magnolia fargesii, has long been used for the treatment of nasal congestion with headache, sinusitis and allergic rhinitis (Jung et al, 1998;Bae, 2000;Kim et al, 2003). Recent studies also revealed that it has antirheumatic and antiangiogenic activities (Kobayashi et al, 1996(Kobayashi et al, , 1998.…”

Section: Introductionmentioning

confidence: 99%

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Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus

Kang

Lee

Han

et al. 2008

Phytotherapy Research

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The present study reports the antiinflammatory activity of a methanol extract isolated from the stem bark of Magnolia kobus (MK). MK potently inhibited lipopolysaccharide (LPS)-induced production of nitric oxide and interleukin-1beta (IL-1beta) in RAW 264.7 cells, a murine macrophage-like cell line. The secretion of tumor necrosis factor-alpha (TNF-alpha) was also suppressed in LPS-stimulated RAW 264.7 cells although the magnitude of inhibition was weaker than that of nitric oxide and IL-1beta. The mRNA expressions of inducible nitric oxide synthase (iNOS), IL-1beta and TNF-alpha were also suppressed by MK in LPS-stimulated RAW 264.7 cells. Further study demonstrated that LPS-induced DNA binding of AP-1 and phosphorylation of c-jun N-terminal kinase (JNK) were inhibited by MK treatment in RAW 264.7 cells, whereas phosphorylation of p38 mitogen-activated protein kinase was unaffected. Moreover, topical application of MK suppressed ear swelling in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation model. Collectively, these results suggest that MK exerts antiinflammatory effects in vitro and in vivo and this might be mediated, at least in part, by blocking AP-1 and JNK activation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus

Kang

Lee

Han

et al. 2008

Phytotherapy Research

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“…As a matter of fact, the antiinflammatory or antiallergic effect of a certain drug was demonstrated to depend on its apoptosis-inducing effect (Fuller et al, 1995;Park et al, 2001;2002;Kim et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

zVAD-fmk, unlike BocD-fmk, does not inhibit caspase-6 acting on 14-3-3/Bad pathway in apoptosis of p815 mastocytoma cells

Yee

Baek²,

Park³

et al. 2006

Exp Mol Med

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In a preliminary study, we found that benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (zVADfmk), unlike Boc-aspartyl(OMe)-fluoromethylketone (BocD-fmk), at usual dosage could not prevent genistein-induced apoptosis of p815 mastocytoma cells. This study was undertaken to reveal the mechanism underlying the incapability of zVAD-fmk in preventing this type of apoptosis. We observed that 14-3-3 protein level was reduced in genistein-treated cells and that BocD-fmk but not zVAD-fmk prevented the reduction of 14-3-3 protein level and the release of Bad from 14-3-3. We also demonstrated that truncated Bad to Bcl-xL interaction in genisteintreated cells was prevented by BocD-fmk but not by zVAD-fmk treatment. Our data indicate that BocDfmk, compared to zVAD-fmk, has a certain preference for inhibiting 14-3-3/Bad signalling pathway. We also elucidated that this differential efficacy of BocD-fmk and zVAD-fmk resulted from the different effect in inhibiting caspase-6 and that co-treatment of zVAD-fmk and caspase-6 specific inhibitor substantially prevented genistein-induced apoptosis. Our data shows that caspase-6 plays a role on Bad/14-3-3 pathway in genistein-induced apoptosis of p815 cells, and that the usual dose of zVAD-fmk, in contrast to BocD-fmk, did not prevent caspase-6 acting on 14-3-3/Bad-mediated event.

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“…The herbal drug has been used for managing nasal conjestion with headache, sinusitis and allergic rhinitis. [1][2] It has also a wide range of pharmacological effects including antirheumatic, 3 antiangiogenic, 4 antiallergic, [5][6][7][8] antiinflammatory, [9][10] and anitmicrobial activities.…”

Section: Introductionmentioning

confidence: 99%

Pattern Recognition of the Herbal Drug, Magnoliae Flos According to their Essential Oil Components

Jeong¹,

Choi²,

Kim³

et al. 2009

Bulletin of the Korean Chemical Society

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This paper describes a pattern recognition method of Magnoliae flos based on a gas chromatographic/mass spectrometric (GC/MS) analysis of the essential oil components. The botanical drug is mainly comprised of the four magnolia species (M. denudata, M. biondii, M. kobus, and M. liliflora) in Korea, although some other species are also being dealt with the drug. The GC/MS separation of the volatile components, which was extracted by the simultaneous distillation and extraction (SDE), was performed on a carbowax column (supelcowax 10; 30 m × 0.25 mm × 0.25 µm) using temperature programming. Variance in the retention times for all peaks of interests was within RSD 2% for repeated analyses (n = 9). Of the 74 essential oil components identified from the magnolia species, approximately 10 major components, which is α-pinene, β-pinene, sabinene, myrcene, d-limonene, eucarlyptol (1,8-cineol), γ-terpinene, p-cymene, linalool, α-terpineol, were commonly present in the four species. For statistical analysis, the original dataset was reduced to the 13 variables by Fisher criterion and factor analysis (FA). The essential oil patterns were processed by means of the multivariate statistical analysis including hierarchical cluster analysis (HCA), principal component analysis (PCA) and discriminant analysis (DA). All samples were divided into four groups with three principal components by PCA and according to the plant origins by HCA. Thirty-three samples (23 training sets and 10 test samples to be assessed) were correctly classified into the four groups predicted by PCA. This method would provide a practical strategy for assessing the authenticity or quality of the well-known herbal drug, Magnoliae flos.

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Magnoliae Flos Induces Apoptosis of RBL-2H3 Cells via Mitochondria and Caspase

Cited by 21 publications

References 19 publications

Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus

Antiinflammatory activity of methanol extract isolated from stem bark of Magnolia kobus

zVAD-fmk, unlike BocD-fmk, does not inhibit caspase-6 acting on 14-3-3/Bad pathway in apoptosis of p815 mastocytoma cells

Pattern Recognition of the Herbal Drug, Magnoliae Flos According to their Essential Oil Components

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