Magnetite-Loaded Carrier Erythrocytes as Contrast Agents for Magnetic Resonance Imaging

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) are commonly used in magnetic resonance imaging (MRI), but their fast phagocytosis makes them less than ideal for this application. To circumvent the lymphocyte-macrophage system, we encapsulated SPIONs into red blood cells (RBCs). For loading, the RBC's membrane was opened by swelling under hypoosmotic conditions and subsequently resealed. In this work, we demonstrate that SPIONs can be loaded into RBCs in a concentration sufficient to obtain strong contrast enhancement in MRI.

Green synthesis of soya bean sprouts-mediated superparamagnetic Fe3O4 nanoparticles

“…Magnetite (Fe 3 O 4 ), as a significant member of magnetic nanomaterials, has recently been subject to extensive research for many practical applications such as catalysis, magnetic storage media, magnetic field assisted separations and analyses, targeted drug delivery, and contrast agents in magnetic resonance imaging (MRI) [1][2][3][4][5][6]. Conventional chemical synthesis by precipitation, high-temperature reactions, sol-gel reactions, decomposition of organometallic precursors, polyol methods, etc.…”

Development of antibody-labelled superparamagnetic nanoparticles for the visualisation of benzo[a]pyrene in porous media with magnetic resonance imaging

“…One possible type of MRI-active NPs, iron oxide NPs, has been used for hyperthermia [7], drug delivery [8], cell separation [9], immunoassays [10,11], bioweapon detection [12] and MRI. Here, iron oxide NPs are used as contrast agents [13] and nanosensors. [14] used iron oxide NPs for the detection of bacteria in milk or blood through MRI.…”

Synthesis of biocompatible hybrid magnetic hollow spheres based on encapsulation strategy

“…Due to their advantages, such as magnetic properties, chemical stability, biocompatibility and low toxicity, magnetic nanocrystals have been intensively studied not only for fundamental scientific interest but also potential applications in biomedical fields, especially in the field of targeted drug delivery (Bráhler et al, 2006;Cao, Zhu, Ma, Li, & Zhang, 2008;Gupta & Gupta, 2005;Hogemann, Ntziachristos, Josephson, & Weissleder, 2002;Hu et al, 2006;Willner & Katz, 2003;Zhao, Kircher, Josephson, & Weissleder, 2002). Pure magnetic nanoparticles are not very useful in practical applications since they are prone to aggregate and rapidly biodegradate when they are directly exposed to a biological system.…”