Magnetic nanoparticles as a drug delivery system that enhance fungicidal activity of polyene antibiotics

Niemirowicz, Katarzyna; Durnaś, Bonita; Tokajuk, Grażyna; Głuszek, Katarzyna; Wilczewska, Agnieszka Z.; Misztalewska, Iwona; Mystkowska, Joanna; Michalak, Grzegorz; Sodo, Anna; Wątek, Marzena; Kiziewicz, Bożena; Góźdż, Stanisław; Głuszek, Stanisław; Bucki, Robert

doi:10.1016/j.nano.2016.07.006

Cited by 68 publications

(51 citation statements)

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“…Ahmad et al (2016) produced a conjugate system with AmB and metal nanoparticles that displayed synergistic antifungal activity due to the antimicrobial property of silver against C. albicans and C. tropicalis . Niemirowicz et al (2016) reported synergistic activity for the combination of polyenes and magnetic nanoparticles. This bio-active nano-sized formulation exhibited enhanced efficiency against two clinical isolates of Candida species in planktonic and biofilm states.…”

Section: New Antifungal Formulations and New Antifungal Drug Structurmentioning

confidence: 99%

Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis

et al. 2017

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The high rates of morbidity and mortality caused by fungal infections are associated with the current limited antifungal arsenal and the high toxicity of the compounds. Additionally, identifying novel drug targets is challenging because there are many similarities between fungal and human cells. The most common antifungal targets include fungal RNA synthesis and cell wall and membrane components, though new antifungal targets are being investigated. Nonetheless, fungi have developed resistance mechanisms, such as overexpression of eﬄux pump proteins and biofilm formation, emphasizing the importance of understanding these mechanisms. To address these problems, different approaches to preventing and treating fungal diseases are described in this review, with a focus on the resistance mechanisms of fungi, with the goal of developing efficient strategies to overcoming and preventing resistance as well as new advances in antifungal therapy. Due to the limited antifungal arsenal, researchers have sought to improve treatment via different approaches, and the synergistic effect obtained by the combination of antifungals contributes to reducing toxicity and could be an alternative for treatment. Another important issue is the development of new formulations for antifungal agents, and interest in nanoparticles as new types of carriers of antifungal drugs has increased. In addition, modifications to the chemical structures of traditional antifungals have improved their activity and pharmacokinetic parameters. Moreover, a different approach to preventing and treating fungal diseases is immunotherapy, which involves different mechanisms, such as vaccines, activation of the immune response and inducing the production of host antimicrobial molecules. Finally, the use of a mini-host has been encouraging for in vivo testing because these animal models demonstrate a good correlation with the mammalian model; they also increase the speediness of as well as facilitate the preliminary testing of new antifungal agents. In general, many years are required from discovery of a new antifungal to clinical use. However, the development of new antifungal strategies will reduce the therapeutic time and/or increase the quality of life of patients.

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Section: New Antifungal Formulations and New Antifungal Drug Structurmentioning

confidence: 99%

Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis

et al. 2017

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“…22 In addition, recent work suggests that the application of nanosystems composed of MNPs and polyene antibiotic may be used for the treatment of infections caused by polyeneresistant fungal strain. 29 In the present study, we evaluate the activity of magnetic nanosystems functionalized with chlorhexidine against clinical isolates of pathogens from different groups including representatives of Gram-positive and Gram-negative bacteria and fungal cells. We demonstrate that covalent attachment of CHX on the magnetic carrier improves their antimicrobial activity compared to a nonimmobilized agent in conditions that mimic the oral cavity environment.…”

mentioning

confidence: 99%

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Tokajuk

Niemirowicz

Deptuła

et al. 2017

IJN

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Nanotechnology offers new tools for developing therapies to prevent and treat oral infections, particularly biofilm-dependent disorders, such as dental plaques and endodontic and periodontal diseases. Chlorhexidine (CHX) is a well-characterized antiseptic agent used in dentistry with broad spectrum activity. However, its application is limited due to inactivation in body fluid and cytotoxicity toward human cells, particularly at high concentrations. To overcome these limitations, we synthesized nanosystems composed of aminosilane-coated magnetic nanoparticles functionalized with chlorhexidine (MNP@CHX). In the presence of human saliva, MNPs@CHX displayed significantly greater bactericidal and fungicidal activity against planktonic and biofilm-forming microorganisms than free CHX. In addition, CHX attached to MNPs has an increased ability to restrict the growth of mixed-species biofilms compared to free CHX. The observed depolarization of mitochondria in fungal cells treated with MNP@CHX suggests that induction of oxidative stress and oxidation of fungal structures may be a part of the mechanism responsible for pathogen killing. Nanoparticles functionalized by CHX did not affect host cell proliferation or their ability to release the proinflammatory cytokine, IL-8. The use of MNPs as a carrier of CHX has great potential for the development of antiseptic nanosystems.

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“…Both nanosystems displayed stronger fungicidal activity than unbound drugs against Candida spp. (Niemirowicz et al, 2016). Nanosystems were more potent than free agents when tested against Candida strains and were able to prevent Candida biofilm formation more effectively with lower hemolytic capacity.…”

Section: Nanopreparations For Polyenesmentioning

confidence: 99%

“…The liposomal system was administered via carotid artery route in SD rats and could improve drug concentration and enhance magnetic targeting to brain tissue in the presence of a magnetic field. Amphotericin B and nystatin magnetic nanoparticles were also designed by Niemirowicz et al (2016). Both nanosystems displayed stronger fungicidal activity than unbound drugs against Candida spp.…”

Section: Nanopreparations For Polyenesmentioning

confidence: 99%

Antifungal Therapy for Systemic Mycosis and the Nanobiotechnology Era: Improving Efficacy, Biodistribution and Toxicity

Souza

Amaral

2017

Front. Microbiol.

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Fungal diseases have been emerging as an important public health problem worldwide with the increase in host predisposition factors due to immunological dysregulations, immunosuppressive and/or anticancer therapy. Antifungal therapy for systemic mycosis is limited, most of times expensive and causes important toxic effects. Nanotechnology has become an interesting strategy to improve efficacy of traditional antifungal drugs, which allows lower toxicity, better biodistribution, and drug targeting, with promising results in vitro and in vivo. In this review, we provide a discussion about conventional antifungal and nanoantifungal therapies for systemic mycosis.

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Magnetic nanoparticles as a drug delivery system that enhance fungicidal activity of polyene antibiotics

Cited by 68 publications

References 50 publications

Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis

Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Antifungal Therapy for Systemic Mycosis and the Nanobiotechnology Era: Improving Efficacy, Biodistribution and Toxicity

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