Novel magnetic ground states have been stabilized in two-dimensional (2D) magnets such as skyrmions, with the potential next-generation information technology. Here, we report the experimental observation of a Néel-type skyrmion lattice at room temperature in a single-phase, layered 2D magnet, specifically a 50% Co–doped Fe
5
GeTe
2
(FCGT) system. The thickness-dependent magnetic domain size follows Kittel’s law. The static spin textures and spin dynamics in FCGT nanoflakes were studied by Lorentz electron microscopy, variable-temperature magnetic force microscopy, micromagnetic simulations, and magnetotransport measurements. Current-induced skyrmion lattice motion was observed at room temperature, with a threshold current density,
j
th
= 1 × 10
6
A/cm
2
. This discovery of a skyrmion lattice at room temperature in a noncentrosymmetric material opens the way for layered device applications and provides an ideal platform for studies of topological and quantum effects in 2D.