Macrostructure of the Tomato Telomeres

Ganal, Martin W.; Lapitan, Nora L. V.; Tanksley, Steven D.

doi:10.2307/3869202

Cited by 19 publications

(21 citation statements)

References 11 publications

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“…FISH showed that this repeat is present in the heterochromatin of all pericentromeres, but not in the nucleolar organizer regions (NORs) (Zhong et al 1996). Three major genomic repeats, TGRI, TGRII and TGRIII, were described by Schweizer et al (1988), Ganal et al (1988Ganal et al ( , 1991 and Lapitan et al (1989Lapitan et al ( , 1991. It was shown that TGRI is located in the distal regions, and that TGRII and TGRIII are located in the pericentromere heterochromatin regions of the chromosomes.…”

Section: Introductionmentioning

confidence: 99%

FISH mapping and molecular organization of the major repetitive sequences of tomato

et al. 2008

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This paper presents a bird_s-eye view of the major repeats and chromatin types of tomato. Using fluorescence insitu hybridization (FISH) with Cot-1, Cot-10 and Cot-100 DNA as probes we mapped repetitive sequences of different complexity on pachytene complements. Cot-100 was found to cover all heterochromatin regions, and could be used to identify repeat-rich clones in BAC filter hybridization. Next we established the chromosomal locations of the tandem and dispersed repeats with respect to euchromatin, nucleolar organizer regions (NORs), heterochromatin, and centromeres. The tomato genomic repeats TGRII and TGRIII appeared to be major components of the pericentromeres, whereas the newly discovered TGRIV repeat was found mainly in the structural centromeres. The highly methylated NOR of chromosome 2 is rich in [GACA] 4 , a microsatellite that also forms part of the pericentromeres, together with [GA] 8 , [GATA] 4 and Ty1-copia. Based on the morphology of pachytene chromosomes and the distribution of repeats studied so far, we now propose six different chromatin classes for tomato: (1) euchromatin, (2) chromomeres, (3) distal heterochromatin and interstitial heterochromatic knobs, (4) pericentromere heterochromatin, (5) functional centromere heterochromatin and (6)

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Section: Introductionmentioning

confidence: 99%

FISH mapping and molecular organization of the major repetitive sequences of tomato

et al. 2008

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“…In tomato, for example, the telomeric repeats are associated with the TGRI satellite repeat at 20 of the 24 chromosome ends, and the distance between the two repeated sequences ranges from a few bp to 150 kb of spacer DNA [1]. Our recent PFGE study on tobacco chromosomes [4] has shown a similar long-range organization of their termini.…”

Section: Introductionmentioning

confidence: 99%

“…The ends of eukaryotic chromosomes consist of many tandemly repeated copies of a DNA sequence (T/A)t.4G ~ 8 [1]. In plants, telomeric sequences were first cloned and sequenced in Arabidopsis thaliana [2].…”

Section: Introductionmentioning

confidence: 99%

The telomeric sequence is directly attached to the HRS60 subtelomeric tandem repeat in tobacco chromosomes

et al. 1995

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PCR and primers derived from the telomeric repeat (CCCTAAA)n and from the tobacco subtelomeric tandemly repetitive sequence HRS60 (EMBL X12489) were used to amplify the region linking the two loci. A 131 bp PCR product was obtained both from total tobacco DNA and from the DNA fraction enriched for telomeres. Its sequence only consists of the telomeric primer and the attached region of the HRS60 repetitive unit up to the end of the sequence complementary to the HRS60 primer. The site of direct continuity between the two sequences is formed by a (dA)7 tract.

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“…The human-type telomere repeat (TTAGGG) n is common in vertebrates (Meyne et al 1989), and it has also been reported in some protozoa and fungi (Blackburn and Gall, 1978, Blackburn and Challoner, 1984, Schechtman, 1990. In plants, the telomere repeat (TTTAGGG) n was isolated in Arabidopsis Ausubel 1988, Richards et al 1992), and this Arabidopsis-type repeat has been reported in many angiosperms and gymnosperms (Ganal et al 1991, Broun et al 1992, Cox et al 1993, Biessmann and Mason 1994, Fuchs et al 1995, Wellinger and Sen 1997, Hizume et al 1998. Allium cepa and A. fistulosum possess unique terminal repetitive sequences and/or rDNA sequences in place of the typical telomere sequence (Pich et al 1996, Pich andSchubert 1998), and the absence of Arabidopsis-type telomere repeats were reported in other Asparagales (Adams et al 2000(Adams et al , 2001.…”

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confidence: 99%

Survey of Arabidopsis- and Human-type Telomere Repeats in Plants Using Fluorescence in situ Hybridisation

Shibata

Hizume

2011

cytologia

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SummaryThe nucleotide sequences of telomere repeats have been identified in many eukaryotes since Blackburn and Gall (1978), and these sequences are specific to the genus or higher taxonomic group. Wide vision telomere sequences are variable across eukaryotes. In plants, Arabidopsis-type telomere repeats (TTTAGGG) n are dominant, although human-type telomere repeats (TTAGGG) n have been reported in a few taxa. Recently, the evolution of these telomere repeats in plants has become the focus of many studies. In this report, the Arabidopsis-type telomere repeat and human-type telomere repeat were surveyed in 87 species of gymnosperms and angiosperms. In 1 gymnosperm species and 62 angiosperm species, fluorescence in situ hybridisation signals of both Arabidopsistype and human-type telomere repeats were detected. Three gymnosperm species and 12 angiosperm species showed only signals of Arabidopsis-type telomere repeats. In 5 angiosperm species, only human-type telomere repeat signals were detected. The co-localisation of Arabidopsis-and humantype telomere repeats on chromosome ends in a wide range of plant species is a novel discovery that may elucidate the evolution of telomere repeats in plants.

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Macrostructure of the Tomato Telomeres

Cited by 19 publications

References 11 publications

FISH mapping and molecular organization of the major repetitive sequences of tomato

FISH mapping and molecular organization of the major repetitive sequences of tomato

The telomeric sequence is directly attached to the HRS60 subtelomeric tandem repeat in tobacco chromosomes

Survey of Arabidopsis- and Human-type Telomere Repeats in Plants Using Fluorescence in situ Hybridisation

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