Macroporous and nanometre scale fibrous PLA and PLA–HA composite scaffolds fabricated by a bio safe strategy

Salerno, Aurelio; Fernández-Gutiérrez, Mar; Barrio, Julio San Román del; Domingo, Concepción

doi:10.1039/c4ra07732f

Cited by 23 publications

(22 citation statements)

References 29 publications

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“…To overcome this limitation, NaCl particles were used in this work as additional porogen during TIPS (Figure 4). The reasons of choosing of NaCl, instead of other more biocompatible material [5,32], are two-fold. First, these particles are insoluble in EL and do not experienced significant chemical and morphological alterations when processed by means of the TIPS technique [44].…”

Section: Discussionmentioning

confidence: 99%

“…The interconnected porosity of the scaffolds was assessed by measuring the volume of water entrapped in the pores of the scaffold using the following equation [31,32]: (1) where m W and m D are the wet (water) and dry masses of the scaffold, ρ W is the density of the water used as intrusion medium and V s is the volume of the scaffold determined by a geometrical calculation using a caliper. The weight measurements were carried out on a high precision balance This effect is particularly evident in the case of neat PLC copolymer, while for the PLC-90 and PLC-50 samples the melting peak area corresponding to the PLA decreased down to 13.7 and 3.5 J/g, respectively, according to the decrease of the PLA fraction into the blends [35,36].…”

Section: Scanning Electron Microscopy (Sem Quanta 200f Feg-esem Feimentioning

confidence: 99%

“…The possibility to use a novel bio-safe TIPS process for preparing nanostructured PCL particles [34] and porous PLA scaffolds [32] by using ethyl lactate (EL) as green biocompatible solvent has been recently explored in our lab. As a further development, the aims of this work are (i) to blend PLA and PLC polymers to control the nano-size scale fibrous structures of porous scaffolds prepared by means of the TIPS; (ii) to combine TIPS and NaCl leaching techniques for achieving a pre-defined network of interconnected large pores; and (iii) to characterize the morphological, structural and in vitro biocompatibility properties of designed scaffolds.…”

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confidence: 99%

“…Biodegradable polyesters, such as PLA, PCL and their copolymers polylactic-co-caprolactone (PLC) are widely used for the fabrication of TE scaffolds with desired morphology and tailored mechanical functionality [3,5,12,[31][32][33]. Furthermore, scaffolds prepared starting from these synthetic materials have good in vitro biocompatibility [5,31,32] and may degrade into non-toxic components with a controllable degradation rate during in vivo implantation [33].…”

mentioning

confidence: 99%

“…Furthermore, scaffolds prepared starting from these synthetic materials have good in vitro biocompatibility [5,31,32] and may degrade into non-toxic components with a controllable degradation rate during in vivo implantation [33].…”

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confidence: 99%

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Bio-safe processing of polylactic-co-caprolactone and polylactic acid blends to fabricate fibrous porous scaffolds for in vitro mesenchymal stem cells adhesion and proliferation

Salerno

Guarino

Oliviero

et al. 2016

Materials Science and Engineering: C

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In this study, the design and fabrication of porous scaffolds, made of blends of polylactic-cocaprolactone (PLC) and polylactic acid (PLA) polymers, for tissue engineering applications is reported. The scaffolds are prepared by means of a bio-safe thermally induced phase separation approach with or without the addition of NaCl particles used as particulate porogen. The scaffolds are characterized to assess their crystalline structure, morphology and mechanical properties, and the texture of the pores and the pore size distribution. Moreover, in vitro human mesenchymal stem cells (hMSCs) culture tests have been carried out to demonstrate the biocompatibility of the scaffolds. The results of this study demonstrate that the nano and macro-structural properties of the scaffolds strongly depend on the polymer crystallization, which, in turn, is affected by the blend composition. Indeed, neat PLC and neat PLA crystallize into globular and randomly arranged nanosize scale fibrous conformations, respectively, thus addressing their pore structure features and mechanical response. Concomitantly, the addition of NaCl particles during the fabrication route allows for the creation of an interconnected network of large pores inside the primary structure.Finally, the results of cell culture tests demonstrate that both the nano and macro-structure of the scaffold affect the in vitro hMSCs adhesion and proliferation.

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Section: Discussionmentioning

confidence: 99%

Section: Scanning Electron Microscopy (Sem Quanta 200f Feg-esem Feimentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Bio-safe processing of polylactic-co-caprolactone and polylactic acid blends to fabricate fibrous porous scaffolds for in vitro mesenchymal stem cells adhesion and proliferation

Salerno

Guarino

Oliviero

et al. 2016

Materials Science and Engineering: C

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The Effect of PLA/HA Coating Thickness on Crack Formation and Corrosion Performance

Hassan

Zamri

Daud

et al. 2022

Advanced Structured Materials

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A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

Hokmabad

Davaran

Aghazadeh

et al. 2018

Tissue Eng Regen Med

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BACKGROUND: The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(e-caprolactone)poly(ethylene glycol)-poly(e-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica (n-SiO 2) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering. METHODS: We evaluated the effect of n-HA and n-SiO 2 incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay. RESULTS: All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with n-SiO 2. While the hydrophilicity of n-SiO 2 incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to n-SiO 2 incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and n-SiO 2. CONCLUSION: Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.

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Macroporous and nanometre scale fibrous PLA and PLA–HA composite scaffolds fabricated by a bio safe strategy

Cited by 23 publications

References 29 publications

Bio-safe processing of polylactic-co-caprolactone and polylactic acid blends to fabricate fibrous porous scaffolds for in vitro mesenchymal stem cells adhesion and proliferation

Bio-safe processing of polylactic-co-caprolactone and polylactic acid blends to fabricate fibrous porous scaffolds for in vitro mesenchymal stem cells adhesion and proliferation

The Effect of PLA/HA Coating Thickness on Crack Formation and Corrosion Performance

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly(ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering

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