Macrophages in degradation of calcium phosphate compound artificial bone: Anin vitro study

Abstract

The isolated mice peritoneal macrophages in degradation of calcium phosphate compound artificial bone--collagen/hydroxylapatite (CHA), hydroxylapatite (HA), beta-tricalcium phosphate (TCP) ceramics, have been studied by use of both Ca++, P concentration assay in cultured supernatant and scanning electron microscopy (SEM). The solubility of Ca++, composition of materials increased more significantly when macrophages were inoculated than when macrophages were not seeded (P < 0.001), and it was shown that the ground materials were wrapped and phagocytized or resorbed extracellularly by macrophages under SEM, suggesting that macrophages could mediate the degradation of calcium phosphate compound artificial bone by phagocytizing and/or degrading extracellularly.

Bioactive Composites Based on Calcium Phosphates for Bone Regeneration

“…In spite of their biocompatibility and bioactivity, CaP ceramics are able to induce an early nonspecific inflammatory reaction due to the presence of ceramic particles that interact with monocytes and macrophages trying to phagocyte them. In fact, macrophages are the major infiltrating cells when HA and TCP are implanted [44]. These cells secrete H+ ions generating an acidic medium that accelerates the degradation of the ceramics [44].…”

“…In fact, macrophages are the major infiltrating cells when HA and TCP are implanted [44]. These cells secrete H+ ions generating an acidic medium that accelerates the degradation of the ceramics [44]. Toxicity of ceramics also depends among other factors on their solubility and on the effect of their degradation by-products [45].…”