Macrophage suspensions prepared from a blood unit for treatment of refractory human ulcers

Zuloff-Shani, Adi; Kachel, Erez; Frenkel, Or; Orenstein, Arie; Shinar, Eilat; Danon, D.

doi:10.1016/j.transci.2003.11.007

Cited by 38 publications

(33 citation statements)

References 29 publications

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“…11,12,14 In brief, a wholeblood unit donated routinely by healthy young donors (age range between 18 to 30 years) was collected into a triple blood-bag system. The unit was then separated into packed red cells, white blood cells (buffy coat), and plasma.…”

Section: Preparation Of Amsmentioning

confidence: 99%

“…19 In addition, in elderly and sick patients with impaired resident macrophage function, injection of allogeneic AMS promotes wound healing and confirms the therapeutic effect of donor AMS. 11,12 Finally, the beneficial effect of allogeneic cells can be explained by the modulation of local immune reactions in response to apoptosis of the transplanted cells. 7 It has been suggested that apoptotic cell ingestion by macrophages induces expression of anti-inflammatory cytokines that could suppress the damage of excessive inflammatory response.…”

Section: Limitationsmentioning

confidence: 99%

“…9,10 In the past few years we have treated Ͼ1500 elderly and sick patients with unhealed ulcers and wounds by local injections of human activated macrophage suspension (AMS). [11][12][13] Based on our favorable clinical experience, we hypothesized that the unique wound healing properties of macrophages could be exploited to promote healing and repair of the infarcted myocardium. Thus, the aim of conducting the present study was to test this hypothesis in a rat model of extensive MI.…”

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confidence: 99%

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Ex Vivo Activated Human Macrophages Improve Healing, Remodeling, and Function of the Infarcted Heart

et al. 2006

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Background-Activated macrophages have a significant role in wound healing and damaged tissue repair. We sought to explore the ability of ex vivo activated macrophages to promote healing and repair of the infarcted myocardium. Methods and Results-Human activated macrophage suspension (AMS) was prepared from a whole blood unit obtained from young donors in a closed sterile system and was activated by a novel method of hypo-osmotic shock. The AMS (Ϸ4ϫ10 5 cells) included up to 43% CD14-positive cells and was injected into the ischemic myocardium of rats (nϭ8) immediately after coronary artery ligation. The control group (nϭ9) was treated with saline injection. The human cells existed in the infarcted heart 4 to 7 days after injection, as indicated by histology, human growth hormone-specific polymerase chain reaction, and magnetic resonance imaging (MRI) tracking of iron oxide-nanoparticle-labeled cells.

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Section: Preparation Of Amsmentioning

confidence: 99%

Section: Limitationsmentioning

confidence: 99%

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confidence: 99%

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Ex Vivo Activated Human Macrophages Improve Healing, Remodeling, and Function of the Infarcted Heart

et al. 2006

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“…Macrophages play an important role in wound healing by producing humoral factors and by increasing fibroplasia, fibrogenesis, and angiogenesis in wounded tissue (10)(11)(12). The decrease in the function of macrophages has been shown to impair wound healing (11).…”

Section: Introductionmentioning

confidence: 99%

“…The decrease in the function of macrophages has been shown to impair wound healing (11). Similarly, enhanced macrophage function and injection of macrophages to the wound have been demonstrated to accelerate wound healing (11,13).…”

Section: Introductionmentioning

confidence: 99%