Macrophage Migration Inhibitory Factor Is Up-Regulated in Human First-Trimester Placenta Stimulated by Soluble Antigen of Toxoplasma gondii, Resulting in Increased Monocyte Adhesion on Villous Explants

Ferro, Eloísa Amália Vieira; Mineo, José Roberto; Ietta, Francesca; Bechi, Nicoletta; Romagnoli, Roberta; Silva, Deise Aparecida Oliveira; Sorda, Giuseppina; Bevilacqua, Estela; Paulesu, Luana

doi:10.2353/ajpath.2008.070432

Cited by 58 publications

(64 citation statements)

References 40 publications

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“…A previous study demonstrated that MIF secretion by first-trimester placental explants is stimulated by soluble antigen of T. gondii alone or with addition of IFN-␥. 25 In the present study, using a model of T. gondii infection, it was demonstrated that MIF discharge was up-regulated in the presence of regulatory cytokines such as IL-10 or TGF-␤1 associated with infection. Accordingly, MIF protein is a unique regulatory mediator with ability to sustain the inflammatory response in the presence of endogenous or exogenous anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 49%

“…A previous study 25 provided evidence that T. gondiisoluble antigen induces production and secretion of MIF by human first-trimester villus explants. The objective of the present study was to investigate the effect of MIF on susceptibility to T. gondii infection in first-and third-trimester human placental explants.…”

mentioning

confidence: 97%

“…MIF has a multifaceted role in immune responses such as phagocytosis, inflammation, and inhibition of neutrophil apoptosis. 19,20 In addition, it is an important factor in determining the outcome of infections caused by protozoan parasites such as Plasmodium chabaudi, 21 P. falciparum, 22 Leishmania major, 23 Trypanosoma cruzi, 24 and T. gondii, 25,26,27 and in helminth (Taenia crassiceps) 28 and bacterial (Salmonella typhimurium) 29 infections.…”

mentioning

confidence: 99%

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Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Gomes¹,

Silva²,

Silva³

et al. 2011

The American Journal of Pathology

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Because macrophage migration inhibitory factor (MIF) is a key cytokine in pregnancy and has a role in inflammatory response and pathogen defense, the objective of the present study was to investigate the effects of MIF in first-and third-trimester human placental explants infected with Toxoplasma gondii. Explants were treated with recombinant MIF, IL-12, interferon-␥, transforming growth factor-␤1, or IL-10, followed by infection with T. gondii RH strain tachyzoites. Supernatants of cultured explants were assessed for MIF production. Explants were processed for morphologic analysis, immunohistochemistry, and real-time PCR analysis. Comparison of infected and stimulated explants versus noninfected control explants demonstrated a significant increase in MIF release in first-trimester but not third-trimester explants. Tissue parasitism was higher in third-than in first-trimester explants. Moreover, T. gondii DNA content was lower in first-trimester explants treated with MIF compared with untreated explants. However, in third-trimester explants, MIF stimulus decreased T. gondii DNA content only at the highest concentration of the cytokine. In addition, high expression of MIF receptor was observed in first-trimester placental explants, whereas MIF receptor expression was low in third-trimester explants. In conclusion, MIF was up-regulated and demonstrated to be important for control of T. gondii infection in first-trimester explants, whereas lack of MIF up-regulation in third-trimester placentas may be involved in higher susceptibility to infection at this gestational age.

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Section: Discussionmentioning

confidence: 49%

mentioning

confidence: 97%

mentioning

confidence: 99%

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Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Gomes¹,

Silva²,

Silva³

et al. 2011

The American Journal of Pathology

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“…6,48 Treatment of human placenta villous explants with soluble antigen from T. gondii plus IFN-g significantly induces parasitized white blood cell adhesion to placental villous cells, augmenting in this way vertical transmission probability. 49,50 Pretreatment of syncytiotrophoblast with the inflammatory cytokines IFN-g, tumor necrosis factor-a (TNF-a) and IL-1a increases the number of monocytes bound to the surface by upregulation of intercellular adhesion molecule 1 (ICAM-1). 49,50 An interesting finding indicates that the parasite may directly adhere to the syncytiotrophoblast through the adhesin called microneme 2 (MIC2), which binds to human ICAM-1.…”

Section: Immune Response In Acquired Toxoplasmosismentioning

confidence: 99%

“…49,50 Pretreatment of syncytiotrophoblast with the inflammatory cytokines IFN-g, tumor necrosis factor-a (TNF-a) and IL-1a increases the number of monocytes bound to the surface by upregulation of intercellular adhesion molecule 1 (ICAM-1). 49,50 An interesting finding indicates that the parasite may directly adhere to the syncytiotrophoblast through the adhesin called microneme 2 (MIC2), which binds to human ICAM-1. 51 Another molecule implicated in this phenomenon is macrophage migration inhibitory factor (MIF), which is produced by first-trimester placental trophoblasts, decidua and cytotrophoblasts, and is implicated in angiogenesis, cell proliferation, and thus in implantation and early embryonic development.…”

Section: Immune Response In Acquired Toxoplasmosismentioning

confidence: 99%

Congenital toxoplasmosis: candidate host immune genes relevant for vertical transmission and pathogenesis

et al. 2010

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Is Toxoplasma gondii type related to clinical outcome in human congenital infection? Systematic and critical review

Rico-Torres

Vargas-Villavicencio

Correa

2016

Eur J Clin Microbiol Infect Dis

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In human congenital toxoplasmosis the effects of parasite burden and pregnancy time at infection on clinical outcome are well known, but there is controversy regarding the role of Toxoplasma gondii type. Through a systematic review of the literature, we aimed to discern if T. gondii type has a role on clinical outcome in human congenital toxoplasmosis. We built up a database of congenital toxoplasmosis from reports of cases, case series and screening-based cohorts, which had information about parasite type, gestation time at maternal infection and/or clinical outcome in the product. Then, we obtained frequencies for loci used to genotype geographical origin of cases and types found. Also, odds ratios were calculated for association between time of maternal infection or parasite type on outcome. Type II parasites were the most common in Europe, Asia and Africa, while in America there were mainly atypical strains. More newborns with clinical problems were born from mothers infected during the first half of gestation than from those acquiring the parasite after week 24, regardless of parasite genotype (92.9 vs. 16.1 %, OR = 67.9, CI95 25.4-181.6). Type I and atypical parasites were associated with clinical problems as opposed to types II and III, regardless of pregnancy period at infection (86.9 vs. 72.9 %, OR = 2.47, CI95 1.1-5.4). A significant and remarkable tendency of type I parasites to be present during early pregnancy was also observed (94.4 vs. 5.6 %, P < 0.009). In addition to parasite burden and period of gestation, T. gondii genotype seems involved in CT clinical outcome.

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Macrophage Migration Inhibitory Factor Is Up-Regulated in Human First-Trimester Placenta Stimulated by Soluble Antigen of Toxoplasma gondii, Resulting in Increased Monocyte Adhesion on Villous Explants

Cited by 58 publications

References 40 publications

Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Effect of Macrophage Migration Inhibitory Factor (MIF) in Human Placental Explants Infected with Toxoplasma gondii Depends on Gestational Age

Congenital toxoplasmosis: candidate host immune genes relevant for vertical transmission and pathogenesis

Is Toxoplasma gondii type related to clinical outcome in human congenital infection? Systematic and critical review

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