Macromolecular Inhibitor of Lymphocyte Transformation in Serum From Patients With Chronic Renal Failure

Nelson, D. S.; Penrose, Jean M.

doi:10.1038/icb.1973.24

Cited by 19 publications

(9 citation statements)

References 3 publications

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“…For example, normal human lymphocytes respond less well to PHA [3, 51, mitogens [6] or allogeneic lymphocytes [7] in the presence of serum from patients with leprosy than in normal human serum. Uremic serum or plasma actively inhibits the response of normal human lymphocytes t o PHA [8,9]. It has been suggested [3] that such effects are due to overproduction of a factor, possibly a n "immunoregulatory a-globulin" [ 10, 1 I ] or "normal immunosuppressive protein" [ 121, whose normal function lies in the homeostasis of immunity, particularly cell-mediated immunity.…”

Section: Introductionmentioning

confidence: 99%

Effect of normal mouse serum on mouse lymphocyte transformation in vitro

Nelson

Shneider

1974

Eur J Immunol

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Mouse spleen cells were cultured in the presence or absence of one of the following mitogens: phytohemagglutinin (PHA), concanavalin A, allogeneic spleen cells or bacterial lipopolysaccharide. The addition of normal mouse serum (NMS) to the cultures usually depressed mitogen-induced lymphocyte transformation as measured by DNA, RNA o r protein synthesis, whether calculated as gross o r net synthesis. The degree of depression was increased as the concentration of NMS serum increased. DNA and RNA synthesis in unstimulated cultures were also usually depressed by NMS. NMS added 24 hours or more after the start of cultures with PHA o r allogeneic lymphocytes was less depressive than serum present from the start and in some cases, actually increased DNA synthesis. The presence of NMS in cultures containing supra-optimal doses of concanavalin A could also increase DNA synthesis. Cells incubated with NMS for 2 4 hours survived as well as cells incubated without NMS, but subsequently responded less well t o PHA. Cells passed through a column of glass wool had a lower baseline DNA synthesis, which was not inhibited by NMS; they responded less well to mitogens and this response was further depressed by NMS.It is suggested that NMS contains a factor which damps the proliferation of mouse T and B cells, without exerting an overt cytotoxic effect, and that it acts directly on lymphocytes.

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Section: Introductionmentioning

confidence: 99%

Effect of normal mouse serum on mouse lymphocyte transformation in vitro

Nelson

Shneider

1974

Eur J Immunol

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“…Several observations suggest that inhibitory factors in uremic serum are responsible for the immunosuppressive effect in chronic renal failure [9,15,19,20,35], The rapid normalization of immune competence after isogeneic renal transplantation supports the hypothesis that the factors are easily excretable. The composition of 'post uremia' rat urine is now studied in our laboratory in search for soluble factors with immunosuppressive ef fects.…”

Section: Discussionmentioning

confidence: 81%

“…Other authors have found a normal in vitro cellular reactivity [12,30] and even an increased PHA stimulation responsiveness of lympho cytes from uremic patients has been reported [7,26]. Humoral factors have been proposed as a cause of im paired immune response by some [10,12,20,22,30] but not all [11] authors.…”

Section: Discussionmentioning

confidence: 99%

The Immunosuppressive Effect of Experimentally Induced Uremia

Konrad

Husberg

1984

Nephron

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Isogeneic BN rats were made uremic by subtotal renal resections. After different periods of uremia the possibility of an immunodeficiency was evaluated by (WF×BN) F₁→BN heterotopic heart transplantations. A prolongation of transplant survival was found that was not related to the duration of uremia. In some rats the uremia was reversed by isogeneic BN→BN kidney transplantation before cardiac grafting. An immediate reversal of the immunodeficiency occurred. Thus an immunosuppressive effect by uremia can be documented, and to some extent quantitated, by heterotopic heart transplantation between rats of different isogeneic strains.

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“…As a result, uraemia may cause an impairment in the differentiation and maturation of thymus lymphocytes. However, there have been many reports on the suppressing activity of uraemic serum on mitogen-induced proliferation of human lymphocytes (Silk, 1967;Newberry & Sanford, 1971;Nelson & Penrose, 1973). Raskova & Morrison (1978) reported the inhibitory effect of sera from uraemic rats in the mixed lymphocyte response.…”

Section: Discussionmentioning

confidence: 99%

Thymus lymphocytes in uraemic rats and the effect of thymosin fraction 5 in vitro

Ikemoto

Kishimoto

Wada

et al. 1990

Clinical and Experimental Immunology

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SUMMARYCellular immunity of thymus lymphocytes in uraemic rats was studied. Severe and moderate uraemia was induced in rats, and sham-operated and normal rats were used as the controls. As a result, the response of thymus lymphocytes to concanavalin A (Con A) significantly decreased in severely uraemic rats, but did not change in moderately uraemic rats. However, when the thymus lymphocytes were pretreated with thymosin fraction 5, the response to Con A was ameliorated in severely uraemic rats. There was a significant correlation between the effect of thymosin fraction 5 on Con A response and Con A response of thymus lymphocytes. In addition, serum from severe uraemic rats suppressed the response of normal thymus lymphocytes to Con A. These results indicate that severe uraemia may cause an impairment in maturation of thymus lymphocytes, which can be improved by thymosin fraction 5 in vitro.Keywords experimentally induced uraemic rats cellular immunity thymus lymphocytes thymosin fraction 5

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Macromolecular Inhibitor of Lymphocyte Transformation in Serum From Patients With Chronic Renal Failure

Cited by 19 publications

References 3 publications

Effect of normal mouse serum on mouse lymphocyte transformation in vitro

Effect of normal mouse serum on mouse lymphocyte transformation in vitro

The Immunosuppressive Effect of Experimentally Induced Uremia

Thymus lymphocytes in uraemic rats and the effect of thymosin fraction 5 in vitro

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