Macro-morphological characterization and kinetics of Mortierella alpina colonies during batch cultivation

Abstract

An effective method for research of macro-morphological characterization and its kinetics was developed by studying the macro-morphological characteristics of Mortierella alpina, an oleaginous zygomycete widely used to produce lipids rich in PUFA, in function of culture medium composition and to link morphological features of fungus with the level of lipid production. A number of distinct morphological forms including hollow pellets, fluffy pellets and freely dispersed mycelia were obtained by changing the fermentation factors. By fitting a Logistic curve, the maximum specific growth rate (μmax)was obtained, which determined the final mycelia morphology. μmax of 0.6584 in three kind of morphological forms is the more appropriate. According to the Luedeking-Piret equation fitting, α≠0 and β≠0, lipid production was partially associated with the hyphal growth, fluffy pellets which turn glucose into lipidwas more effective than the other two kinds of morphological forms.

Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models

“…With the exception of maximum specific growth rate, the altered kinetic values (i.e., m S , k dD , and k dL ) found in the engineered A. oryzae strain when grown in different culture media (NaNO 3 -defined and CSH/ML media) indicated that the lipid metabolites are dynamic molecules in leveraging several cellular metabolisms during the cell growth, probably for maintaining cell homeostasis. It can be documented that the generated logistic equation could explained well the fungal growth, which was similar to the previous studies of other oleaginous fungi and yeast (Amirsadeghi et al, 2015;Fang et al, 2018;Xu et al, 2018). In addition, the Luedeking-Piret equation, which was modified by including the term of lipid degradation, could describe the phenomena of lipid production and degradation as well as DGLA production.…”

Optimization of the Fermentative Production of Rhizomucor miehei Lipase in Aspergillus oryzae by Controlling Morphology

“…In general, the morphology of filamentous fungi could be divided into three types: dispersed mycelia, clumps, and dense pellets [ 12 ]. Moreover, the final products can exhibit significant differences in the optimal morphology [ 13 , 14 ]. For example, pellets are considered to be the optimal form in the production of glucoamylase by A. niger .…”