m6A mRNA Modification as a New Layer of Gene Regulation in Plants

Kim, Jiwoo; Shim, Sangrea; Lee, Hong-Woo; Seo, Pil Joon

doi:10.1007/s12374-020-09239-5

Cited by 17 publications

(9 citation statements)

References 48 publications

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“…The transcriptional regulation of stress responses includes DNA methylation, as well as the involvement of multiple transcription factors (Sanchez and Paszkowski, 2014; Yang and Guo, 2018). The post‐transcriptional regulation of the stress response is mediated by diverse RNA‐binding proteins (Kang et al , 2013; Lee and Kang, 2016), and in this respect RNA methylation has been proposed as a new layer of post‐transcriptional gene regulation associated with the stress response in plants (Arribas‐Hernández and Brodersen, 2020; Hu et al , 2019; Kim et al , 2020; Miao et al , 2020; Yue et al , 2019; Zheng et al , 2021). A recent study has demonstrated differential mRNA m 6 A methylation in response to salt stress in Arabidopsis , which suggests a close correlation between m 6 A methylation and the salt stress response (Anderson et al , 2018).…”

Section: Introductionmentioning

confidence: 99%

N⁶‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

et al. 2021

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As the most abundant internal modification of mRNA, N 6 -methyladenosine (m 6 A) methylation of RNA is emerging as a new layer of epitranscriptomic gene regulation in cellular processes, including embryo development, flowering-time control, microspore generation and fruit ripening, in plants. However, the cellular role of m 6 A in plant responses to environmental stimuli remains largely unexplored. In this study, we show that m 6 A methylation plays an important role in salt stress tolerance in Arabidopsis. All mutants of m 6 A writer components, including MTA, MTB, VIRILIZER (VIR) and HAKAI, displayed salt-sensitive phenotypes in an m 6 A-dependent manner. The vir mutant, in which the level of m 6 A was most highly reduced, exhibited salt-hypersensitive phenotypes. Analysis of the m 6 A methylome in the vir mutant revealed a transcriptomewide loss of m 6 A modification in the 3ʹ untranslated region (3ʹ-UTR). We demonstrated further that VIR-mediated m 6 A methylation modulates reactive oxygen species homeostasis by negatively regulating the mRNA stability of several salt stress negative regulators, including ATAF1, GI and GSTU17, through affecting 3ʹ-UTR lengthening linked to alternative polyadenylation. Our results highlight the important role played by epitranscriptomic mRNA methylation in the salt stress response of Arabidopsis and indicate a strong link between m 6 A methylation and 3ʹ-UTR length and mRNA stability during stress adaptation.

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Section: Introductionmentioning

confidence: 99%

N⁶‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

et al. 2021

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“…This tool will help to uncover methylated RNA transcripts at the whole-cell level (Figure 4). CRISPR-based m 6Amodification tools enable epitranscriptome editing (Kim et al, 2020) and are designed to read (m 6Abinding proteins), write (methyltransferase complex), and erase (demethylases) the m 6A at targeted sites for crop quality improvement (Zheng et al, 2020). Overall, the emergence of such novel CRISPR-based RNA editing tools will allow researchers to edit specific RNAs, which will help in the study of uncharted RNA biology.…”

Section: Crispr-based Rna Editingmentioning

confidence: 99%

CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

et al. 2021

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The central dogma (CD) of molecular biology is the transfer of genetic information from DNA to RNA to protein. Major CD processes governing genetic flow include the cell cycle, DNA replication, chromosome packaging, epigenetic changes, transcription, posttranscriptional alterations, translation, and posttranslational modifications. The CD processes are tightly regulated in plants to maintain genetic integrity throughout the life cycle and to pass genetic materials to next generation. Engineering of various CD processes involved in gene regulation will accelerate crop improvement to feed the growing world population. CRISPR technology enables programmable editing of CD processes to alter DNA, RNA, or protein, which would have been impossible in the past. Here, an overview of recent advancements in CRISPR tool development and CRISPR-based CD modulations that expedite basic and applied plant research is provided. Furthermore, CRISPR applications in major thriving areas of research, such as gene discovery (allele mining and cryptic gene activation), introgression (de novo domestication and haploid induction), and application of desired traits beneficial to farmers or consumers (biotic/abiotic stress-resilient crops, plant cell factories, and delayed senescence), are described. Finally, the global regulatory policies, challenges, and prospects for CRISPR-mediated crop improvement are discussed.

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“…Posttranscriptional regulation of RNA metabolism, which includes intron splicing, precursor‐RNA processing, RNA turnover, and translational control, is a pivotal process of stress responses, and it is mediated by diverse RNA‐binding proteins (Kang et al, 2013; Lee & Kang, 2016; Marondedze et al, 2019). In recent years, RNA methylation is emerging as a new layer in posttranscriptional gene regulation associated with plant stress responses (Anderson et al, 2018; Arribas‐Hernández & Brodersen, 2020; Hu et al, 2019; Huong et al, 2020; Kim et al, 2020; Miao et al, 2020; Yue et al, 2019; Zheng et al, 2021). However, the cellular role of m 6 A writers and erasers in plant stress responses and the molecular mechanisms underlying m 6 A‐mediated stress responses are largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Alpha‐ketoglutarate‐dependent dioxygenase homolog 10B, an N⁶‐methyladenosine mRNA demethylase, plays a role in salt stress and abscisic acid responses in Arabidopsis thaliana

Shoaib

Manduzio

et al. 2021

Physiologia Plantarum

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N 6 -methyladenosine (m 6 A) is an abundant methylation mark in eukaryotic mRNAs. It is installed and removed by methyltransferases ("writers") and demethylases ("erasers"), respectively. A recent study has demonstrated that alpha-ketoglutaratedependent dioxygenase homolog 10B (ALKBH10B) is an mRNA m 6 A eraser affecting floral transition in Arabidopsis thaliana. However, the roles of m 6 A eraser proteins, including ALKHB10B, in plant adaptation to abiotic stresses are largely unknown. In this study, we aimed to determine the role of ALKBH10B in the response of A. thaliana to abiotic stresses and abscisic acid (ABA). The m 6 A level increased in response to salt stress, and m 6 A levels in alkbh10b mutants were higher than those in the wild-type under both normal and salt stress conditions. Germination of alkbh10b mutant seeds was markedly delayed under salt stress but not under dehydration, cold, or ABA conditions. Seedling growth and survival rate of alkbh10b mutants were enhanced under salt stress. Notably, salt-tolerant phenotypes of alkbh10b mutants were correlated with decreased levels of several m 6 A-modified genes, including ATAF1, BGLU22, and MYB73, which are negative effectors of salt stress tolerance. In response to ABA, both seedling and root growth of alkbh10b mutants were inhibited via upregulating ABA signaling-related genes, including ABI3 and ABI4. Collectively, these findings indicate that ALKBH10B-mediated m 6 A demethylation affects the transcript levels of stress-responsive genes, which are important for seed germination, seedling growth, and survival of Arabidopsis thaliana in response to salt stress or ABA.

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m6A mRNA Modification as a New Layer of Gene Regulation in Plants

Cited by 17 publications

References 48 publications

N⁶‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

N⁶‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

Alpha‐ketoglutarate‐dependent dioxygenase homolog 10B, an N⁶‐methyladenosine mRNA demethylase, plays a role in salt stress and abscisic acid responses in Arabidopsis thaliana

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m6A mRNA Modification as a New Layer of Gene Regulation in Plants

Cited by 17 publications

References 48 publications

N6‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

N6‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

Alpha‐ketoglutarate‐dependent dioxygenase homolog 10B, an N6‐methyladenosine mRNA demethylase, plays a role in salt stress and abscisic acid responses in Arabidopsis thaliana

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N⁶‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

N⁶‐Methyladenosine mRNA methylation is important for salt stress tolerance in Arabidopsis

Alpha‐ketoglutarate‐dependent dioxygenase homolog 10B, an N⁶‐methyladenosine mRNA demethylase, plays a role in salt stress and abscisic acid responses in Arabidopsis thaliana