M-cadherin and β-catenin participate in differentiation of rat satellite cells

Wróbel, Edyta; Brzóska, Edyta; Moraczewski, Jerzy

doi:10.1016/j.ejcb.2006.11.004

Cited by 39 publications

(34 citation statements)

References 56 publications

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“…The canonical pathway involves the stabilisation of β-catenin, which then translocates to the nucleus to control transcription by means of the T-cell factor (TCF)/lymphocyte enhancement factor (LEF) family of transcription factors (Willert and Jones, 2006). β-catenin is expressed by adult rat myogenic cells (Ishido et al, 2006;Wrobel et al, 2007) and by mouse C2 cells (Goichberg et al, 2001). Constitutive expression of β-catenin or inhibition of endogenous protein degradation both result in a greater proportion of Pax7-positive MyoD-negative satellite cells, and a decreased proportion of differentiating cells.…”

Section: Wnt Signalling In Controlling Satellite-cell Functionmentioning

confidence: 99%

All muscle satellite cells are equal, but are some more equal than others?

Zammit

2008

Journal of Cell Science

182

160

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Skeletal muscle is an accessible adult stem-cell model in which differentiated myofibres are maintained and repaired by a selfrenewing stem-cell compartment. These resident stem cells, which are known as satellite cells, lie on the surface of the muscle fibre, between the plasmalemma and overlying basal lamina. Although they are normally mitotically quiescent in adult muscle, satellite cells can be activated when needed to generate myoblasts, which eventually differentiate to provide new myonuclei for the homeostasis, hypertrophy and repair of muscle fibres, or fuse together to form new myofibres for regeneration. Satellite cells also self-renew in order to maintain a viable stem-cell pool that is able to respond to repeated demand. The study of the control of self-renewal has led to the idea that the satellite-cell pool might be heterogeneous: that is it might contain both self-renewing satellite 'stem' cells and myogenic precursors with limited replicative potential in the same anatomical location. The regulatory circuits that control satellite-cell self-renewal are beginning to be deciphered, with Pax7, and Notch and Wnt signalling being clearly implicated. This Commentary seeks to integrate these interesting new findings into the wider context of satellite-cell biology, and to highlight some of the many outstanding questions. SummaryAll muscle satellite cells are equal, but are some more equal than others? Peter S. ZammitKingʼs College London, Randall Division of Cell and Molecular Biophysics, New Huntʼs House, Guyʼs Campus, London SE1 1UL, UK Author for correspondence (e-mail: peter.zammit@kcl.ac.uk) Accepted 24 July 2008 Journal of Cell Science 121, 2975-2982 Published by The Company of Biologists 2008Biologists doi:10.1242 Journal of Cell Science 2976 DNA replication during S phase by using tritiated thymidine suggested that labelled satellite cells give rise to myonuclei following cell division (Moss and Leblond, 1970); this was later confirmed by many other studies. Importantly, the tracking of DNA replication also indicated that satellite-cell divisions could be asymmetric in growing muscle and give rise to both myonuclei and satellite cells, which suggests that satellite cells self-renew (Moss and Leblond, 1971). The satellite-cell population in growing muscle can be separated into two groups: a fast-dividing population that undergoes limited replication before differentiating, and a slow-dividing population that might return to G0 between cycles and give rise to the fast-dividing population (Schultz, 1996).Together, these data suggest that, during the early postnatal period, fast-dividing satellite cells initially undergo asymmetric divisions to produce both myonuclei for muscle growth and satellite cells, but later undergo symmetric divisions, so that few of the fastdividing cells remain as satellite cells in adult muscle. The bulk of the satellite cells that exist in adults, therefore, presumably derives from the slow-dividing population. This raises the question of whether most satellite ce...

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Section: Wnt Signalling In Controlling Satellite-cell Functionmentioning

confidence: 99%

All muscle satellite cells are equal, but are some more equal than others?

Zammit

2008

Journal of Cell Science

182

160

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“…One of the members of the cadherin family, M-cadherin, has been found during embryogenesis in myogenic cells of somitic origin and in adult muscles. We investigated the distribution and function of Mcadherin and β-catenin during differentiation of myoblasts in primary cultures of rat satellite cells (Wrobel et al, 2007). First, we noticed that M-cadherin with β-catenin localized between fusing myoblasts and that β-catenin colocalized with actin in pre-fusing myoblasts (Fig.…”

Section: D B Amentioning

confidence: 99%

From Planarians to Mammals - the many faces of regeneration

Moraczewski

Archacka²,

Brzóska³

et al. 2008

Int. J. Dev. Biol.

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This report presents the history of the involvement of the Department of Cytology in studies of different aspects of regeneration. It can be divided into two major phases; the first focused on the regeneration of Turbellarians and the second on the regeneration of rat skeletal muscles including the differentiation of satellite cells in vitro. Regeneration of Turbellarians was investigated both at the cellular and molecular levels including the role of the protein kinase C (PKC) in this process. Studies on skeletal muscle regeneration initially focused on factors involved in regulation of signal transduction pathways. Next, we explored the influence of growth factors on the modulation of the regeneration process. Another important aspect of our studies was investigating of the distribution and function of different proteins involved in adhesion and fusion of myoblasts. Finally, we are also conducting research on the role of stem cells from other tissues in the regeneration of skeletal muscle. KEY WORDS: regeneration, planaria, skeletal muscleIn this report we present the history of the involvement of the Department of Cytology team in the studies on regeneration dated from 1974. This research may be divided into two major phases. First, we concentrated our efforts on the regeneration of Turbellarians. Then, in 1987, we switched our research model to the regeneration of the rat skeletal muscle and the differentiation of satellite cells in vitro. In this article we demonstrate the main topics of the studies conducted solely by the members of our group or in collaboration with other laboratories. Planarian regenerationPlanarians serve as a good model for the study of cell differentiation. Due the presence of totipotent stem cells they are characterized by an unique ability to regenerate. These stem cells (neoblasts) are responsible for the regeneration and growth. Neoblasts are true totipotent stem cells, like embryonic stem cells in vertebrates. They can be involved in the differentiation of all planarian tissues. Turbellarian of the order Catenulida were cultured in our laboratory for 6 years and served those days as a subject of various studies (Fig. 1). Our first publication in that we focused at Catenula concerned its asexual reproduction and regeneration (Moraczewski, 1977). The examination of the ultrastructure of the myodermal body wall by (Moraczewski and Czubaj, 1974) and (Soltynska et al., 1976), as well as of the nervous system by (Moraczewski et al., 1977a, Moraczewski et Int. J. Dev. Biol. 52: 219-227 (2008) ., 1977b), made it possible to trace precisely the course of regeneration and paratomy i.e. transverse fission during which differentiation of new organs occurred prior to separation from the mother animal ( Fig. 1 A,B). These studies showed that fissioning in Catenula was a typical paratomy as defined by (Wagner, 1890). The Catenula species analyzed by us always divided into only two zooids, and fission always occurred behind the intestine. The opisthe i.e. he posterior daughter org...

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“…It has been described that Mcad interacts with Bcat (Kuch et al, 1997;Wróbel et al, 2007) so we decided to analyze potential correlations between the expression of Bcat, Mcad, Ki67 and dystrophin. We found that Bcat and Mcad always colocalize at the contact area between the myofiber and quiescent (Fig.…”

Section: Contact Zones Without Dystrophin Do Not Show Plasma Membranementioning

confidence: 99%

“…However, mice with a null mutation in Mcad show no muscle defects, probably because of compensation by the other cadherins (Hollnagel et al, 2002). It has been described that the cytoplasmatic tails of Mcad interacts with beta-catenin (Bcat), and that both proteins are essential for the differentiation of the fusing myoblasts (Kuch et al, 1997;Wróbel et al, 2007). Moreover, different studies suggest a role for Bcat in controlling the transcription of target genes in proliferating satellite cells, in addition to its role in myoblast fusion.…”

Section: Introductionmentioning

confidence: 99%

Mcad-mediated intercellular interactions activate satellite cell division

Martí

Montserrat

Pardo

et al. 2013

Journal of Cell Science

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SummaryAdult muscle stem cells and their committed myogenic precursors, commonly referred to as the satellite cell population, are involved in both muscle growth after birth and regeneration after damage. It has been previously proposed that, under these circumstances, satellite cells first become activated, divide and differentiate, and only later fuse to the existing myofiber through M-cadherin-mediated intercellular interactions. Our data show that satellite cells fuse with the myofiber concomitantly to cell division, and only when the nuclei of the daughter cells are inside the myofiber, do they complete the process of differentiation. Here we demonstrate that Mcadherin plays an important role in cell-to-cell recognition and fusion, and is crucial for cell division activation. Treatment of satellite cells with M-cadherin in vitro stimulates cell division, whereas addition of anti-M-cadherin antibodies reduces the cell division rate. Our results suggest an alternative model for the contribution of satellite cells to muscle development, which might be useful in understanding muscle regeneration, as well as muscle-related dystrophies.

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M-cadherin and β-catenin participate in differentiation of rat satellite cells

Cited by 39 publications

References 56 publications

All muscle satellite cells are equal, but are some more equal than others?

All muscle satellite cells are equal, but are some more equal than others?

From Planarians to Mammals - the many faces of regeneration

Mcad-mediated intercellular interactions activate satellite cell division

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