Lysosomal Labilization: Rapid, Target-Specific Effect of ACTH

Szego, Clara M.; Rakich, Douglas R.; Seeler, Barbara J.; Gross, Rosemarie Steadman

doi:10.1210/endo-95-3-863

Cited by 23 publications

(10 citation statements)

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“…When 'purified' nuclei were stripped of all superficial adherent material of cytoplasmic origin by subjecting them to the cold detergent procedure outlined above [29,30], the resultant 'ultrapurified' samples from hormone-pretreated animals still exhibit- in 'purified' nuclear preparations from preputial gland after estrogen pretreatment in vivo. Methods of preparation of the ovariectomized rats were as described in the text.…”

Section: Persistence Of Lysosomal Enzymes In ' Ultrapurified' Nucleimentioning

confidence: 99%

Intranuclear Concentration of Lysosomal Hydrolases in Steroid Target Cells

Szego¹,

Steadman²,

Seeler³

1974

European Journal of Biochemistry

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The present investigation was designed to determine whether the rapid intracellular redistribution of lysosomes to a perinuclear position in reproductive target cells after administration of specific gonadal hormones in vivo was accompanied by evidence of penetration of the nucleoplasm by lysosomal marker enzymes. Nuclei were prepared by conventional isolation and purification procedures from preputial glands or uteri that were excised from gonadectornized rats within 2-15 min after intravenous injection of physiological doses of estradiol-17/3, testosterone or saline control solutions. Control and experimental preparations were essentially "pure", as judged by routine phasecontrast microscopic criteria in general use. However, nuclei from target cells of animals given hormone contained substantial activities of representative lysosomal hydrolases, including acid phosphatase, /3-glucuronidase and acid ribonuclease 11. In contrast, the samples originating from the target tissue of saline-treated controls or from non-target tissues contained minimal concentrations of these enzyme activities. After further purification of the nuclei by removal of the outer nuclear membrane through brief exposure to very low concentrations of Triton X-100 in the cold, the resultant 'ultrapurified' nuclei retained significant concentrations of structurally latent lysosomal marker enzymes after hormonal pretreatment without evidence of appreciable contamination by enzymes of mitochondria1 origin. Similar results were obtained with nuclei isolated by non-aqueous procedures. These combined observations appear to exclude adsorption artifacts. The total activities of lysosomal enzymes in the unfractionated homogenates and the MgZ+-dependent ribonuclease activity indigenous to the nuclear compartment were unaffected by prior hormone treatment. These results are consistent with the hypothesis that invasion of the nucleoplasm of specific target cells by lysosomal products may serve as a mechanism for triggering genic derepression in steroid hormone-induced growth.

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Section: Persistence Of Lysosomal Enzymes In ' Ultrapurified' Nucleimentioning

confidence: 99%

Intranuclear Concentration of Lysosomal Hydrolases in Steroid Target Cells

Szego¹,

Steadman²,

Seeler³

1974

European Journal of Biochemistry

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“…Very recent work reveals a parallel lysosomal translocation phenomenon in vaginal cells of the estrogen-treated ovariectomized rat and also in oocytes within very short intervals of their treatment in vitro with low concentra tions of LH [41]. Similar observations are available for perinuclear lyso somal accumulation in adrenocortical cells of hypophysectomized animals [87]. In contrast, this phenomenon is absent in nontarget cells [84, 85.…”

Section: Figmentioning

confidence: 71%

“…4), is elicited by the specific tropic hormone in vivo [84,87,94] and in vitro [56]. These findings have a counterpart in the effect of vasopressin in epithelial cells isolated from amphibian blad der [54,55], thus suggesting rather broad application of this response in the propagation of the primary interaction of hormone at the cell mem brane.…”

Section: Function Of Lysosomal Cathepsin(s) In Transformation Of Intrmentioning

confidence: 80%

“…The entry of lysosomal marker enzymes into the nuclear compartment, even when the nuclei have been rendered devoid of all morphologic evidence of ad herent cytoplasmic elements by removal of the outer nuclear membrane ('ultrapurified'), is illustrated in figure 6 and more extensively elsewhere [S3, 96], Such penetration of the nucleus by lysosomal components, a rapid by product of site-specific lysosome activation by tropic hormone, is not con fined to the steroids. As seen in figure 7, invasion of the nuclear compart ment by lysosomal hydrolases and, concomitantly, presumably also by acidic matricular proteins of these organelles, is also elicited by ACTH in its adrenocortical target cells [87]. The substantial evidence suggesting that the action of peptidal hormones need not be confined exclusively to the plasmalemmal locus is presented in detail elsewhere [85].…”

Section: Figmentioning

confidence: 94%

“…cortex [87], and ovary ( fig. 4), is elicited by the specific tropic hormone in vivo [84,87,94] and in vitro [56].…”

Section: Function Of Lysosomal Cathepsin(s) In Transformation Of Intrmentioning

confidence: 99%

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Steroid-Protein Binding: from Circulating Blood to Target Cell Nucleus

Szego

1976

Gynecol Obstet Invest

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The concept of protein binding of steroid hormones is traced from its origins through the period of confirmation and initial extension. Further considerations include cell surface recognition sites, subcellular sources of binding protein, and the new evidence that lysosomes participate in the translocation and intranuclear penetration of the hormone in steroid target cells. This short review thus aims to provide a link between current investigations and the ideas upon which they have been based.

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Parallels in the Modes of Action of Peptide and Steroid Hormones: Membrane Effects and Cellular Entry

Szego

1978

Structure and Function of the Gonadotropins

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Lysosomal Labilization: Rapid, Target-Specific Effect of ACTH

Cited by 23 publications

References 0 publications

Intranuclear Concentration of Lysosomal Hydrolases in Steroid Target Cells

Intranuclear Concentration of Lysosomal Hydrolases in Steroid Target Cells

Steroid-Protein Binding: from Circulating Blood to Target Cell Nucleus

Parallels in the Modes of Action of Peptide and Steroid Hormones: Membrane Effects and Cellular Entry

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