2017
DOI: 10.1155/2017/9850398
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Abstract: Before this study, there had been no research evaluating the relationship between a lysosomal exoglycosidase profile and secretory function in the salivary glands of rats with streptozotocin- (STZ-) induced type 1 diabetes. In our work, rats were divided into 4 groups of 8 animals each: control groups (C2, C4) and diabetic groups (STZ2, STZ4). The secretory function of salivary glands—nonstimulated and stimulated salivary flow, α-amylase, total protein—and salivary exoglycosidase activities—N-acetyl-β-hexosami… Show more

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Cited by 27 publications
(39 citation statements)
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“…It is well known that α-amylase is one of the most important salivary proteins. It is also a recognized biomarker of salivary gland damage [35,67]. During stimulation, over 60% of saliva is produced by parotid glands [33,59].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…It is well known that α-amylase is one of the most important salivary proteins. It is also a recognized biomarker of salivary gland damage [35,67]. During stimulation, over 60% of saliva is produced by parotid glands [33,59].…”
Section: Discussionmentioning
confidence: 99%
“…To assess the salivary gland function, the activity of salivary amylase was also determined. A colorimetric method with 3,5-dinitrosalicylic acid was used and absorbance was measured at 540 nm [35]. The activity of salivary amylase was performed in triplicates.…”
Section: Saliva Collectionmentioning
confidence: 99%
“…Mitochondrial isolation buffer (250 mmol/L sucrose, 5 mmol/L Tris-HCl, 2 mmol/L ethylene glycol bis(2-aminoethyl)tetraacetic acid (EGTA), pH 7.4) was prepared in ultra-pure water (Sigma-Aldrich, Germany) immediately before homogenization [3,4]. To prevent sample proteolysis, protease inhibitors were added (Complete Mini, Roche, Mannheim, Germany; 1 tablet/10 mL of the isolation buffer) [22]. Homogenates were centrifuged at 500× g (10 min, 4 • C) and the resulting supernatant was centrifuged twice at 8000× g (10 min, 4 • C).…”
Section: Mitochondria Isolationmentioning
confidence: 99%
“…Homogenates were centrifuged at 500× g (10 min, 4 • C) and the resulting supernatant was centrifuged twice at 8000× g (10 min, 4 • C). The mitochondria pellet was resuspended in ice-cold mitochondria isolation buffer and used immediately [18,22,23]. The purity of the mitochondrial fraction was assessed by Western blotting.…”
Section: Mitochondria Isolationmentioning
confidence: 99%
“…Considering that oxidative stress plays a key role in HF pathogenesis [5][6][7], secretory dysfunction of salivary glands may also occur in HF patients, similarly to other diseases with proven etiology of oxidative stress. Indeed, a relationship between oxidative stress and impaired salivary gland function has been demonstrated in obesity [23,24], insulin resistance [25,26], type 1 diabetes [27], and psoriasis [28]. Therefore, it is necessary to assess the salivary redox parameters in patients with HF, as well as the secretory function of the salivary glands.…”
Section: Introductionmentioning
confidence: 99%