Lysosomal enzyme release in hypothermically perfused dog kidneys

Pavlock, Gail S.; Southard, James H.; Starling, James R.; Belzer, Folkert O.

doi:10.1016/0011-2240(84)90050-6

Cited by 26 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also observed good correlation between our DTIBC values and values calculated from the sum of serum iron and UIBC, but the calculated TIBC values were lower than the DTIBC-determined values and TIBC values obtained by an automated assay. Similar results have been reported in a comparison of a calculation method and a direct automated TIBC assay (16 ). In the present study, we reevaluated the relationships between TRF concentrations and TIBC values obtained by the DTIBC assay and by calculation methods in a large sample (n ϭ 188) and used…”

supporting

confidence: 77%

Use of Two Biomarkers of Renal Ischemia to Assess Machine-perfused Non-Heart-beating Donor Kidneys

et al. 2003

View full text Add to dashboard Cite

supporting

confidence: 77%

Use of Two Biomarkers of Renal Ischemia to Assess Machine-perfused Non-Heart-beating Donor Kidneys

et al. 2003

View full text Add to dashboard Cite

“…Thus, loss of microvillar enzymes äs well äs intracellular and extracellular release of lysosomal enzymes, both described in kidney cryopreservation (26,27), do not occur uniformly. However, the outcome of the transplantation does not seem to depend on intact brush-border membranes, äs suggested from alkaline phosphatase activities in proximal tubules of intraoperative biopsies A t , B t , and Dj.…”

Section: Discussionmentioning

confidence: 97%

Catalytic Activities of Alkaline Phosphatase and N-Acetyl-ß-D-Glucosaminidase in Human Cortical Nephron Segments: Heterogeneous Changes in Acute Renal Failure and Acute Rejection Following Kidney Allotransplantation

Schmid¹,

Mall²,

Bockhorn³

1986

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Summary:The catalytic activities of alkaline phosphatase and N-acetyl-ß-Z>-glucosaminidase, constituents of luminal bnish-border membranes and lysosomes of kidney tubular cells, were measured in human kidney allografts in the mäintenance and recovery phases of acute renal failure and in acute rejection crisis. The enzyme activities were fluorometrically determined in single microdissected cortical nephron Segments of biopsies from 4 kidney allografts taken intraoperatively and postoperatively at different periods, which exhibited either goöd function or dysfunction. For comparison, the unaffected part of a human kidney nephrectomized due to hypernephroma äs well äs a biopsy of a morphologically normal human kidney were examined.Both enzymes displayed highest activities in the proximal part of the human nephron. In some intraoperative and ppstoperätive biopsies with acute renal failure, alkaline phosphatase activity was reduced in proximal tubules, predominantly in the straight portion. This reduction could not be correlated with function. In acute rejection, very low alkaline phosphatase activities were uniformly found in proximal convoluted and straight tubules. Fürthermore, intraoperative biopsies and biopsies of the functioning allograft have only approximately 50% of normal N-acetyl-ß-D-glucosaminidase activity in proximal convoluted tubules, but generally normal values in the straight portion. However, in acute renal failure, this enzyme activity was several-fold enhanced along the whole nephron, when compared with intraoperative values. In acute rejection, N-acetyl-ß-jp-glucpsaminidase activity was slightly reduced in proximal convoluted tubules, when compared with biopsies showing gööd function.It is suggested that the decrease of proximal tubular enzyme activities is the consequence of increased enzymuria and inadequate enzyme regeneration. On the other hand, the overshoot of N-acetyl-ß-/)-glucosamißidase activity in the maintenance phase of acute renal failure appears to indicate increased degradative capacity, associated with cellular regeneration along the whole nephron. Schmid, Mall and Bockhorn: Enzyme activities in human kidney ailografts with impaired function Kataly tische Aktivitäten von alkalischer Phosphatase undN-Acetyl-ß-D-glucosaminidase in menschlichen corticalen Nephronsegmenten: Heterogene Veränderungen bei akutem Nierenversagen und akuter Abstoßung nach NierentransplantationZusammenfassung: Die katalytischen Aktivitäten von alkalischer Phosphatase und N-Acetyl-ß-Z>-glucosaminidase, Bestandteilen von luminalen Bürstensaum-Membranen und Lysosomen der Nierentubulus-Zellen, wurden in menschlichen Nierentransplantaten in Spät-und Erholungsphase akuten Nierenversagens und bei akuter Abstoßungskrise gemessen. Die Enzymaktivitäten wurden fluorometrisch in einzelne^mikrodissezier-ten corticalen Nephronsegmenten von Biopsien aus 4 Nierentransplantaten bestimmt, die intraoperativ und postoperativ zu verschiedenen Zeiten bei guter oder schlechter Funktion gewonnen wurden. Zum Vergleich wurden der n...

show abstract

“…LC3-positive signals, LER, and large vacuoles were accumulated in the cathepsin D-deficient renal tubular cells. As lysosomal proteases, cathepsin D, cathepsin B and cathepsin L are abundantly expressed in the kidneys [22,23,24]. Only cathepsin D is significantly increased after renal ischemia/reperfusion injury [14].…”

Section: Discussionmentioning

confidence: 99%

Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury

Suzuki

Tanida

Ohmuraya

et al. 2019

IJMS

View full text Add to dashboard Cite

Cathepsin D is one of the major lysosomal aspartic proteases that is essential for the normal functioning of the autophagy-lysosomal system. In the kidney, cathepsin D is enriched in renal proximal tubular epithelial cells, and its levels increase during acute kidney injury. To investigate how cathepsin D-deficiency impacts renal proximal tubular cells, we employed a conditional knockout CtsDflox/−; Spink3Cre mouse. Immunohistochemical analyses using anti-cathepsin D antibody revealed that cathepsin D was significantly decreased in tubular epithelial cells of the cortico-medullary region, mainly in renal proximal tubular cells of this mouse. Cathepsin D-deficient renal proximal tubular cells showed an increase of microtubule-associated protein light chain 3 (LC3; a marker for autophagosome/autolysosome)-signals and an accumulation of abnormal autophagic structures. Renal ischemia/reperfusion injury resulted in an increase of early kidney injury marker, Kidney injury molecule 1 (Kim-1), in the cathepsin D-deficient renal tubular epithelial cells of the CtsDflox/−; Spink3Cre mouse. Inflammation marker was also increased in the cortico-medullary region of the CtsDflox/−; Spink3Cre mouse. Our results indicated that lack of cathepsin D in the renal tubular epithelial cells led to an increase of sensitivity against ischemia/reperfusion injury.

show abstract

Lysosomal enzyme release in hypothermically perfused dog kidneys

Cited by 26 publications

References 13 publications

Use of Two Biomarkers of Renal Ischemia to Assess Machine-perfused Non-Heart-beating Donor Kidneys

Use of Two Biomarkers of Renal Ischemia to Assess Machine-perfused Non-Heart-beating Donor Kidneys

Catalytic Activities of Alkaline Phosphatase and N-Acetyl-ß-D-Glucosaminidase in Human Cortical Nephron Segments: Heterogeneous Changes in Acute Renal Failure and Acute Rejection Following Kidney Allotransplantation

Lack of Cathepsin D in the Renal Proximal Tubular Cells Resulted in Increased Sensitivity against Renal Ischemia/Reperfusion Injury

Contact Info

Product

Resources

About