Lysine and threonine plasma concentrations in Ivorian patients living with human immunodeficiency virus

Boyvin, Lydie; SÃ©ri, KiprÃ© Laurent; AkÃ, Jeanne Armande; Djaman, Joseph Allico

doi:10.5897/jahr2017.0438

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…In the present study, we have been applying different concentrations of Lys from 0.5 to 10 mM in both C2C12 myotubes and 3T3-L1 adipocytes. Physiologic concentration of Lys has been reported about 201 to 253 μM according to the type of food intake [34] or 92 ± 6 μM [35]. We used Lys above physiologic concentration and there was no toxicity against the studied cells.…”

Section: Discussionmentioning

confidence: 99%

L-lysine protects C2C12 myotubes and 3T3-L1 adipocytes against high glucose damages and stresses

et al. 2019

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Hyperglycemia is a hallmark of diabetes, which is associated with protein glycation and misfolding, impaired cell metabolism and altered signaling pathways result in endoplasmic reticulum stress (ERS). We previously showed that L-lysine (Lys) inhibits the nonenzymatic glycation of proteins, and protects diabetic rats and type 2 diabetic patients against diabetic complications. Here, we studied some molecular aspects of the Lys protective role in high glucose (HG)-induced toxicity in C2C12 myotubes and 3T3-L1 adipocytes. C2C12 and 3T3-L1 cell lines were differentiated into myotubes and adipocytes, respectively. Then, they were incubated with normal or high glucose (HG) concentrations in the absence/presence of Lys (1 mM). To investigate the role of HG and/or Lys on cell apoptosis, oxidative status, unfolded protein response (UPR) and autophagy, we used the MTT assay and flow cytometry, spectrophotometry and fluorometry, RT-PCR and Western blotting, respectively. In both cell lines, HG significantly reduced cell viability and induced apoptosis, accompanying with the significant increase in reactive oxygen species (ROS) and nitric oxide (NO). Furthermore, the spliced form of X-box binding protein 1 (XBP1), at both mRNA and protein levels, the phosphorylated eukaryotic translation initiation factor 2α (p-eIf2α), and the Light chain 3 (LC3)II/LC3I ratio was also significantly increased. Lys alone had no significant effects on most of these parameters; but, treatment with HG plus Lys returned them all to, or close to, the normal values. The results indicated the protective role of Lys against glucotoxicity induced by HG in C2C12 myotubes and 3T3-L1 adipocytes.

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Section: Discussionmentioning

confidence: 99%

L-lysine protects C2C12 myotubes and 3T3-L1 adipocytes against high glucose damages and stresses

et al. 2019

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The additive effect of type 2 diabetes on fibrinogen, von Willebrand factor, tryptophan and threonine in people living with HIV

et al. 2019

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Seasonal Variations in the Biological Value of Kyrgyz Donkey’s Milk Proteins

Turganbaeva,

Musulmanova,

Kydyraliev

2023

Food Processing: Techniques and Technology

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Due to its protein component, donkey’s milk is a multifunctional product with a wide range of beneficial physiological properties. It is known for its antioxidant, anti-inflammatory, rejuvenating, antimicrobial, and antitumor effects. However, the milk of Kyrgyz donkeys has not received proper scientific attention in this respect. This study analyzed the seasonal amino acid profile and biological value of milk obtained from Kyrgyz donkeys. The donkeys belonged to the Kyrgyz breed and inhabited the highlands of the Chui Region, Republic of Kyrgyzstan. The sampling took place in April, July, and September. The amino acid composition of milk proteins was determined by capillary electrophoresis. The biological value of proteins was assessed by amino acid score, index of essential amino acids (Auxerre index), utility ratio of essential amino acids, and utility ratio of amino acid composition. The analysis revealed 18 amino acids, including eight essential ones. The total content of amino acids in the spring samples was 1840.07 mg/100 g. In the summer samples, this value peaked at 2037 mg/100 g; in autumn, it dropped to 1264.35 mg/100 g. The changes could be explained by the seasonal differences in the diet. The ratio of essential to non-essential amino acids approached 1:1. The milk also proved to contain arginine, which is an essential amino acid for a juvenile organism. In the spring samples, its content was 2.5 times as big as in the autumn ones. Leucine and isoleucine were responsible for 1/3 of the essential amino acids (322.60 ± 19.35 mg/100 g). The summer samples demonstrated the maximal content of some other essential amino acids, except for valine and tryptophan, which appeared to be the limiting essential amino acids for donkey’s milk in the autumn period. Total essential amino acids in the spring, summer, and autumn samples amounted to 54.24, 59.26, and 34.77 g/100 g, respectively, which exceeded the value for the reference protein (27.46 g/100 g). Kyrgyz donkey’s milk proteins were of high biological value, regardless of the season, which means that donkey’s milk can become part of new functional products.

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Lysine and threonine plasma concentrations in Ivorian patients living with human immunodeficiency virus

Cited by 3 publications

References 28 publications

L-lysine protects C2C12 myotubes and 3T3-L1 adipocytes against high glucose damages and stresses

L-lysine protects C2C12 myotubes and 3T3-L1 adipocytes against high glucose damages and stresses

The additive effect of type 2 diabetes on fibrinogen, von Willebrand factor, tryptophan and threonine in people living with HIV

Seasonal Variations in the Biological Value of Kyrgyz Donkey’s Milk Proteins

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