Lymphocytes modulate peritoneal leukocyte recruitment in peritonitis

Kipari, Tiina; Watson, Sandra; Houlberg, Kris; Lepage, Stephanie E.; Hughes, Jeremy; Cailhier, Jean‐François

doi:10.1007/s00011-009-0019-5

Cited by 12 publications

(10 citation statements)

References 19 publications

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“…While the initial cells mediating the inflammation over the first 24 h consist primarily of neutrophils, macrophages predominate the peritoneal infiltrate after 3 days [25]. At later time points, T-cells and B-cells play important roles in the immune response, but are detected at lower levels [26]. Here, we used this model of thioglycollate-induced inflammation to evaluate the influence of NEC in suppressing a macrophage-mediated inflammatory response.…”

Section: Resultsmentioning

confidence: 99%

Oral Administration of Nano-Emulsion Curcumin in Mice Suppresses Inflammatory-Induced NFκB Signaling and Macrophage Migration

Young¹,

Bruss²,

Gardner³

et al. 2014

PLoS ONE

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Despite the widespread use of curcumin for centuries in Eastern medicine as an anti-inflammatory agent, its molecular actions and therapeutic viability have only recently been explored. While curcumin does have potential therapeutic efficacy, both solubility and bioavailability must be improved before it can be more successfully translated to clinical care. We have previously reported a novel formulation of nano-emulsion curcumin (NEC) that achieves significantly greater plasma concentrations in mice after oral administration. Here, we confirm the immunosuppressive effects of NEC in vivo and further examine its molecular mechanisms to better understand therapeutic potential. Using transgenic mice harboring an NFκB-luciferase reporter gene, we demonstrate a novel application of this in vivo inflammatory model to test the efficacy of NEC administration by bioluminescent imaging and show that LPS-induced NFκB activity was suppressed with NEC compared to an equivalent amount of curcumin in aqueous suspension. Administration of NEC by oral gavage resulted in a reduction of blood monocytes, decreased levels of both TLR4 and RAGE expression, and inhibited secretion of MCP-1. Mechanistically, curcumin blocked LPS-induced phosphorylation of the p65 subunit of NFκB and IκBα in murine macrophages. In a mouse model of peritonitis, NEC significantly reduced macrophage recruitment, but not T-cell or B-cell levels. In addition, curcumin treatment of monocyte derived cell lines and primary human macrophages in vitro significantly inhibited cell migration. These data demonstrate that curcumin can suppress inflammation by inhibiting macrophage migration via NFκB and MCP-1 inhibition and establish that NEC is an effective therapeutic formulation to increase the bioavailability of curcumin in order to facilitate this response.

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Section: Resultsmentioning

confidence: 99%

Oral Administration of Nano-Emulsion Curcumin in Mice Suppresses Inflammatory-Induced NFκB Signaling and Macrophage Migration

Young¹,

Bruss²,

Gardner³

et al. 2014

PLoS ONE

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“…In this study, we observed the influence of manual acupuncture on peritonitis. Peritonitis is defined as inflammation of the peritoneum, from any cause, that spreads throughout the abdomen and might involve many types of cells, including resident macrophages, which play a critically important role in the orchestration of neutrophil recruitment to the peritoneal cavity [9, 10]. Thus, for this purpose, we evaluated the effect of SP6 acupuncture on an experimental animal model of carrageenan-induced peritonitis and also investigated mechanisms responsible for its anti-inflammatory effects.…”

Section: Introductionmentioning

confidence: 99%

Involvement of Interleukin‐10 in the Anti‐Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

Silva

Guginski

Werner

et al. 2011

Evidence-Based Complementary and Alternative Medicine

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In this study, we determined the anti-inflammatory effect of manual acupuncture at the Sanyinjiao or Spleen 6 (SP6) point on carrageenan-induced peritonitis in mice and investigated mechanisms that may underlie this effect. In the first set of experiments, male Swiss mice were allocated into five groups: the control (sterile saline), dexamethasone (DEXA), invasive sham-acupuncture (non-acupoint), SP6 acupuncture and carrageenan-treated groups. Ten minutes after needle retention or 30 min after DEXA treatment, mice received an intraperitoneal injection of carrageenan (750 μg/mouse). After 4 h, total leukocyte and differential cell counts (neutrophils and mononuclear), myeloperoxidase (MPO) activity, vascular permeability and cytokine levels were evaluated. In another set of experiments, adrenalectomized (ADX) mice were used to study the involvement of the adrenal gland on the therapeutic effects of acupuncture. Mice were allocated into two groups: the ADX and sham-operated animals (Sham ADX) that were subdivided into four subgroups each: the control (sterile saline), DEXA, SP6 acupuncture and carrageenan-treated groups. The SP6 and DEXA treatments inhibited the inflammatory cell infiltration, vascular permeability and MPO activity in carrageenan-injected mice. In addition, the SP6 treatment also increased interleukin (IL)-10 levels. In contrast, when the animals were adrenalectomized, the SP6 treatment failed to reduce total leukocyte and the plasma extravasation. In conclusion, this study clearly demonstrates the anti-inflammatory effect of SP6 acupuncture in a model of carrageenan-induced peritonitis. Our results demonstrated that SP6 acupuncture depends of the adrenal glands and increased IL-10 levels to produce its anti-inflammatory action.

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“…T cells would act to limit leukocyte recruitment with an absence of T cells facilitating increased PMN and monocyte/MΦ recruitment. B cells would play a role in promoting MΦ recruitment in the later phase of inflammation as both RAG-1 KO mice and µMT mice exhibited reduced monocyte/macrophage (MΦ) recruitment at late time points [14]. …”

Section: Lymphocytesmentioning

confidence: 99%

Regulation of Experimental Peritonitis: A Complex Orchestration

Laurin

Brissette

Lepage

et al. 2012

Nephron Exp Nephrol

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Experimental peritonitis is a frequently used inflammatory model to evaluate leukocyte recruitment. By the intrinsic characteristics of the peritoneal cavity, the various resident cell populations have a role to play in the initiation, the modulation and the resolution of peritoneal inflammation. Through various manipulations of these cell populations, we gained important knowledge on their respective roles in peritoneal inflammation. In this brief review, we will focus on the cellular regulation of leukocyte recruitment in experimental peritonitis.

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Lymphocytes modulate peritoneal leukocyte recruitment in peritonitis

Abstract: These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.

Cited by 12 publications

References 19 publications

Oral Administration of Nano-Emulsion Curcumin in Mice Suppresses Inflammatory-Induced NFκB Signaling and Macrophage Migration

Oral Administration of Nano-Emulsion Curcumin in Mice Suppresses Inflammatory-Induced NFκB Signaling and Macrophage Migration

Involvement of Interleukin‐10 in the Anti‐Inflammatory Effect of Sanyinjiao (SP6) Acupuncture in a Mouse Model of Peritonitis

Regulation of Experimental Peritonitis: A Complex Orchestration

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