Lymphocyte subpopulations in peripheral blood and malignant effusions of cancer patients

Robinson, Eliezer; Segal, Ricardo; Vesely, Z.; Mekori, T.

doi:10.1016/0277-5379(86)90029-5

Cited by 15 publications

(9 citation statements)

References 5 publications

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“…Malignant pleural fluid has been studied in the past as if it were a single entity, and the cellular and cytokine components have generally not been studied by grouping them according to tumor type. [27][28][29] For example, a recent study by Atanackovic et al examined adhesion molecules and chemokine receptors on lymphocytes from 16 malignant effusions due to eight different tumor types. 27 Here, we demonstrate that there are significant differences in the regulatory and activated lymphoid components of pleural effusions secondary to mesothelioma as compared to those secondary to NSCLC or breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Regulatory T cells and cytokines in malignant pleural effusions secondary to mesothelioma and carcinoma

DeLong

Carroll²,

Henry³

et al. 2005

Cancer Biology & Therapy

108

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Immunotherapy against a variety of malignancies, including pleural-based malignancies, has shown promise in animal models and early human clinical trials, but successful efforts will need to address immunosuppressive factors of the tumor and host, particularly certain cytokines and CD4(+) CD25(+) regulatory T cells (Treg). Here, we evaluated the cellular and cytokine components of malignant pleural effusions from 44 patients with previously diagnosed mesothelioma, non-small cell lung cancer (NSCLC), or breast cancer and found significant differences in the immune profile of pleural effusions secondary to mesothelioma vs. carcinoma. Although a high prevalence of functionally suppressive CD4(+) CD25(+) T cells was found in carcinomatous pleural effusions, mesothelioma pleural effusions contained significantly fewer CD4(+) CD25(+) T cells. Activated CD8(+) T cells in pleural fluid were significantly more prevalent in mesothelioma than carcinoma. However, there is clear patient-to-patient variability and occasional mesothelioma patients with high percentages of CD4(+) CD25(+) pleural effusion T cells and low percentages of CD8(+) CD25(+) pleural effusion T cells can be identified. Mesothelioma pleural effusions contained the highest concentrations of the immunosuppressive cytokine transforming growth factor (TGF)-beta. Thus, the contribution of cellular and cytokine components of immunosuppression associated with malignant pleural effusions varies by tumor histology and by the individual patient. These results have implications for the development of immunotherapy directed to the malignant pleural space, and suggest the need to tailor immunotherapy to overcome immunosuppressive mechanisms in tumor environments.

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Section: Discussionmentioning

confidence: 99%

Regulatory T cells and cytokines in malignant pleural effusions secondary to mesothelioma and carcinoma

DeLong

Carroll²,

Henry³

et al. 2005

Cancer Biology & Therapy

108

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“…The highly elevated pleural fluid slL-2R values in TB and malignant pleurisy seems to be due to a high percentage o f activated lymphocytes in these effu sions [19][20][21]. It may reflect the activation o f local im mune status.…”

Section: Discussionmentioning

confidence: 99%

Neopterin, Soluble lnterleukin-2 Receptor and Adenosine Deaminase Levels in Pleural Effusions

Chiang

Chiang²,

Lin³

et al. 1994

Respiration

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We evaluated soluble interleukin-2 receptors (sIL-2R), neopterin and adenosine deaminase (ADA) in pleural effusions from 93 patients with tuberculosis, malignancies, uremia, pneumonia and other kinds of pleurisy. There were significantly elevated ADA (102.7 ± 47 U/1) and sIL-2R (8,238 ± 4,117 U/ml) values in tuberculous (TB) pleural fluids as compared with other non-TB pleural fluids (p < 0.005). The neopterin levels in pleural fluid were significantly lower in the cancer group (17.3 ± 7.8 nmol/l; p < 0.005) and most strikingly elevated (309.4 ± 112.2 nmol/l; p < 0.0001) in patients with uremic pleural effusions. Using cut-off values of 60 U/l in ADA and 5,000 U/l in sIL-2R, 92.0 and 86.9% of pleural effusions were TB in origin. Eighty-four percent of patients with malignant pleural effusions had neopterin levels less than 25 nmol/l.

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“…The test was carried out as described by Reinherz et al 4 Mononuclear cells were obtained by the standard fractionation Hypaque gradient centrifugation technique. Helper and suppressor cells were identified by using three murine monoclonal antibodies (Ortho Pharmaceuticals, Raritan, NJ): CD3 for mature T lymphocytes; CD4 for helper inducer cells, and CD8 for suppressor cytotoxic (method described previously 5 ). The appropriate monoclonal antibody was added to aliquots of 1-2 ϫ 10 6 cells and the cells were stained by an immune fluorescent technique using a fluorescein-labeled goat antimouse: immunoglobulin (Ig)G fluorescein isothiocyanate-conjugated goat antimouse IgG antibody (Tago Inc., Burlingame, CA).…”

Section: Methodsmentioning

confidence: 99%

“…The appropriate monoclonal antibody was added to aliquots of 1-2 ϫ 10 6 cells and the cells were stained by an immune fluorescent technique using a fluorescein-labeled goat antimouse: immunoglobulin (Ig)G fluorescein isothiocyanate-conjugated goat antimouse IgG antibody (Tago Inc., Burlingame, CA). 5 T-cell subsets distribution was evaluated using the flow cytometer (FacScan, Becton-Dickinson, San Jose, CA).…”

Section: Methodsmentioning

confidence: 99%

Lymphocyte subpopulations in patients with multiple primary tumors

Robinson

Segal

Struminger

et al. 1999

Cancer

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Lymphocyte subpopulations in peripheral blood and malignant effusions of cancer patients

Cited by 15 publications

References 5 publications

Regulatory T cells and cytokines in malignant pleural effusions secondary to mesothelioma and carcinoma

Regulatory T cells and cytokines in malignant pleural effusions secondary to mesothelioma and carcinoma

Neopterin, Soluble lnterleukin-2 Receptor and Adenosine Deaminase Levels in Pleural Effusions

Lymphocyte subpopulations in patients with multiple primary tumors

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