Luteolin enhances cholinergic activities in PC12 cells through ERK1/2 and PI3K/Akt pathways

Omri, Abdelfatteh El; Han, Jianzhong; Kawada, Kunio; Abdrabbah, Manef Ben; Isoda, Hiroko

doi:10.1016/j.brainres.2011.12.019

Cited by 38 publications

(25 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistently, the present study also showed that PACA might induce NGF expression in the entire midbrain cell population including astrocytes. On the other hand, NGF induced sustained activation of ERK to drive neurite outgrowth [46]. NGF activated the Akt and ERK pathways in a timingdependent manner to support the initiation and extension of neurites [14].…”

Section: Discussionmentioning

confidence: 99%

N-Propargyl Caffeamide (PACA) Ameliorates Dopaminergic Neuronal Loss and Motor Dysfunctions in MPTP Mouse Model of Parkinson’s Disease and in MPP+-Induced Neurons via Promoting the Conversion of proNGF to NGF

et al. 2017

View full text Add to dashboard Cite

Insufficient production of nerve growth factor (NGF) is implicated in Parkinson's disease (PD). We recently discovered that caffeic acid derivative N-propargyl caffeamide (PACA) not only potentiated NGF-induced neurite outgrowth but also attenuated 6-hydroxydopamine neurotoxicity in neuronal culture. The aim of the present study was to investigate whether PACA could increase NGF levels against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) neurotoxicity in a mouse PD model. We induced parkinsonism in mice by intraperitoneal injection of MPTP for seven consecutive days. Animal motor functions were assessed by rotarod test and pole test. Our results showed that PACA ameliorated motor impairments in MPTP-challenged mice. Based on Western blot analysis and/or immunofluorescence staining of NGF and tyrosine hydroxylase (TH), PACA preserved TH levels in the midbrain substantia nigra pars compacta. PACA also increased NGF expression while it decreased proNGF accumulation. Interestingly, NGF was widely induced in the midbrains including astrocytes. To elucidate the mechanisms by which PACA induces NGF, we focused on the effects of PACA on two neurotrophic signaling pathways, the PI3K and MEK pathways. We found that PACA induced the phosphorylation of Akt, ERK, and CREB against MPTP-mediated alterations. Importantly, PACA increased NGF levels and subsequently induced TrkA activation in MPTP-treated mice. Consistently, PACA also increased NGF levels in dopaminergic PC12 cells and primary rat midbrain neurons against N-methyl-4-phenylpyridinium iodide (MPP) toxicity. ERK and PI3K inhibitors attenuated the effects of PACA on NGF levels. Collectively, our results suggest that PACA may rescue NGF insufficiency via sequential activation of PI3K/Akt, ERK1/2, and CREB signaling pathways. Graphical Abstract ᅟ.

show abstract

Section: Discussionmentioning

confidence: 99%

N-Propargyl Caffeamide (PACA) Ameliorates Dopaminergic Neuronal Loss and Motor Dysfunctions in MPTP Mouse Model of Parkinson’s Disease and in MPP+-Induced Neurons via Promoting the Conversion of proNGF to NGF

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Addition of U0126 significantly abolished the curcumin-induced neuritogenesis in PC-12 cells; the percentage of neurite bearing cells was reduced from 21.6 ± 2.4% to 4.1 ± 1.6%, moreover, the phosphorylation of ERK1/2 (Thr202/Tyr204) was also inhibited [47]. A study by El Omri et al (2012) [68] showed that U0126 and LY294002 attenuated luteolin- (a neuroactive compound isolated from Rosmarinus officinalis ) induced acetylcholinesterase (neuronal differentiation marker) and neuritogenic activity in PC-12 cells. El Omri et al (2012) [68] concluded that the activation of ERK1/2 and AKT signaling were involved in the neuritogenic and cholinergic activities of luteolin.…”

Section: Discussionmentioning

confidence: 99%

6-shogaol, a neuroactive compound of ginger (jahe gajah) induced neuritogenic activity via NGF responsive pathways in PC-12 cells

Seow

Hong

Lee

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

Background: Ginger is a popular spice and food preservative. The rhizomes of the common ginger have been used as traditional medicine to treat various ailments. 6-Shogaol, a pungent compound isolated from the rhizomes of jahe gajah (Zingiber officinale var officinale) has shown numerous pharmacological activities, including neuroprotective and anti-neuroinflammatory activities. The aim of this study was to investigate the potential of 6-shogaol to mimic the neuritogenic activity of nerve growth factor (NGF) in rat pheochromocytoma (PC-12) cells.

show abstract

“…Luteolin is the most abundant flavonoid in plants such as rosemary, celery, and parsley (Chowdhury et al, 2002; El Omri et al, 2012). It has been shown that luteolin has several pharmacological properties including a protective role of DNA against hydrogen peroxide-induced toxicity and anti-inflammatory actions (Cheng et al, 2010; El Omri et al, 2012).…”

Section: Flavonoidsmentioning

confidence: 99%

“…It has been shown that luteolin has several pharmacological properties including a protective role of DNA against hydrogen peroxide-induced toxicity and anti-inflammatory actions (Cheng et al, 2010; El Omri et al, 2012). …”

Section: Flavonoidsmentioning

confidence: 99%

Nutraceuticals in cognitive impairment and Alzheimerâ€™s disease

et al. 2014

View full text Add to dashboard Cite

Several chemical substances belonging to classes of natural dietary origin display protective properties against some age-related diseases including neurodegenerative ones, particularly Alzheimer’s disease (AD). These compounds, known as nutraceuticals, differ structurally, act therefore at different biochemical and metabolic levels and have shown different types of neuroprotective properties. The aim of this review is to summarize data from observational studies, clinical trials, and randomized clinical trials (RCTs) in humans on the effects of selected nutraceuticals against age-related cognitive impairment and dementia. We report results from studies on flavonoids, some vitamins and other natural substances that have been studied in AD and that might be beneficial for the maintenance of a good cognitive performance. Due to the substantial lack of high-level evidence studies there is no possibility for recommendation of nutraceuticals in dementia-related therapeutic guidelines. Nevertheless, the strong potential for their neuroprotective action warrants further studies in the field.

show abstract

Luteolin enhances cholinergic activities in PC12 cells through ERK1/2 and PI3K/Akt pathways

Cited by 38 publications

References 37 publications

N-Propargyl Caffeamide (PACA) Ameliorates Dopaminergic Neuronal Loss and Motor Dysfunctions in MPTP Mouse Model of Parkinson’s Disease and in MPP+-Induced Neurons via Promoting the Conversion of proNGF to NGF

N-Propargyl Caffeamide (PACA) Ameliorates Dopaminergic Neuronal Loss and Motor Dysfunctions in MPTP Mouse Model of Parkinson’s Disease and in MPP+-Induced Neurons via Promoting the Conversion of proNGF to NGF

6-shogaol, a neuroactive compound of ginger (jahe gajah) induced neuritogenic activity via NGF responsive pathways in PC-12 cells

Nutraceuticals in cognitive impairment and Alzheimerâ€™s disease

Contact Info

Product

Resources

About