“…Bruno et al confirmed this observation in a study of the total surfactant pool and also showed that reduced surfactant PC was not attributable to dehydration, and could not be prevented by supplementation with choline, despite some evidence of compromised choline status. 12 Calorie deprivation also resulted in a n altered quantity of pulmonary phospholipids. The content of all phospholipids of both the extracellular and intracellular surfactant fractions decreased after 4 8 hours on the diet, except for PG.…”