Luminescent Sensing of Oxygen Using a Quenchable Probe and Upconverting Nanoparticles

Achatz, Daniela E.; Meier, Robert J.; Fischer, Lorenz H.; Wolfbeis, Otto S.

doi:10.1002/anie.201004902

Cited by 228 publications

(108 citation statements)

References 50 publications

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“…First, the sensor is self-referencing, as the ratio of the reference to the sensing dye represents a parameter that is independent of dye concentration or variations with instrumentation. Second, coupling the OSD and NQD mixture within a biologically inert and O 2 permeable polymer matrix improves luminescent stability and provides possibilities for alternative sensing modalities using multiphoton microcopy techniques (McLaurin et al 2009, Achatz et al 2011, Amelia et al 2011). Third, in contrast with polarographic techniques, our molecular sensor does not consume the O 2 it seeks to measure.…”

Section: Discussionmentioning

confidence: 99%

FRET excited ratiometric oxygen sensing in living tissue

Ingram

Zhang

2013

Journal of Neuroscience Methods

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Dynamic analysis of oxygen (O2) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glass substrates allowed the development of a series of optical sensors able to measure interstitial oxygen concentration [O2] with several hundred millisecond temporal resolution in varying biological microdomains of active brain tissue.

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Section: Discussionmentioning

confidence: 99%

FRET excited ratiometric oxygen sensing in living tissue

Ingram

Zhang

2013

Journal of Neuroscience Methods

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“…The use of UCNPs for gas molecules sensing has attracted extensive interest because of their outstanding properties 28, 94. The first sensor based on UCNPs of oxygen was presented by Wolfbeis group 94, in which UCNPs as nanolamps and iridium(III) complexes as quenchers were both embedded in a thin film of ethyl cellulose. The emission of the UCNPs overlaps with the absorbance of the iridium(III) complex (Figure 12A).…”

Section: Sensing Using Re-doped Upconversion Nanoparticlesmentioning

confidence: 99%

Sensing Using Rare-Earth-Doped Upconversion Nanoparticles

Hao¹,

Chen²,

Yang³

2013

Theranostics

177

123

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Optical sensing plays an important role in theranostics due to its capability to detect hint biochemical entities or molecular targets as well as to precisely monitor specific fundamental psychological processes. Rare-earth (RE) doped upconversion nanoparticles (UCNPs) are promising for these endeavors due to their unique frequency converting capability; they emit efficient and sharp visible or ultraviolet (UV) luminescence via use of ladder-like energy levels of RE ions when excited at near infrared (NIR) light that are silent to tissues. These features allow not only a high penetration depth in biological tissues but also a high detection sensitivity. Indeed, the energy transfer between UCNPs and biomolecular or chemical indicators provide opportunities for high-sensitive bio- and chemical-sensing. A temperature-sensitive change of the intensity ratio between two close UC bands promises them for use in temperature mapping of a single living cell. In this work, we review recent investigations on using UCNPs for the detection of biomolecules (avidin, ATP, etc.), ions (cyanide, mecury, etc.), small gas molecules (oxygen, carbon dioxide, ammonia, etc.), as well as for in vitro temperature sensing. We also briefly summarize chemical methods in synthesizing UCNPs of high efficiency that are important for the detection limit.

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“…The 2 nd decade of the 21 st century is expected to witness even greater success in exploring potential applications of f-UCNP, such as multimodal targeted imaging of various diseases/pathways/targets, sensitive detection of circulating tumor cells, stem cell labeling and in vivo tracking, non-invasive real time therapeutic effect monitoring, among others. With the already demonstrated use of f-UCNP as nano-sensors for sensing temperature in a single cell [134], surrounding oxygen concentration [135], intracellular mercury ions and glutathione [136, 137], and avidin [37, 138], it is expected that not only multifunctional but also “smart” nano-devices based on f-UCNP will emerge in the near future.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Functionalized Upconversion Nanoparticles: Versatile Nanoplatforms for Translational Research

Chen¹,

Bu²,

Cai³

et al. 2013

CMM

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The design, application, and translation of targeted multimodality molecular imaging probes based on nanotechnology has attracted increasing attentions during the last decade and will continue to play vital roles in cancer diagnosis and personalized medicine. With the growing awareness of drawbacks of traditional organic dyes and quantum dots, biocompatible lanthanide ion doped upconversion nanoparticles have emerged as promising candidates for clinically translatable imaging probes, owing to their unique features that are suitable for future targeted multimodal imaging in living subjects. In this review, we summarized the recent advances in the field of functionalized upconversion nanoparticles (f-UCNP) for biological imaging and therapy in vivo, and discussed the future research directions, obstacles ahead, and the potential use of f-UCNP in translational research.

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Luminescent Sensing of Oxygen Using a Quenchable Probe and Upconverting Nanoparticles

Cited by 228 publications

References 50 publications

FRET excited ratiometric oxygen sensing in living tissue

FRET excited ratiometric oxygen sensing in living tissue

Sensing Using Rare-Earth-Doped Upconversion Nanoparticles

Functionalized Upconversion Nanoparticles: Versatile Nanoplatforms for Translational Research

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