Luminescent iridium(III) complexes as novel protein staining agents

Jia, Junli; Hao, Fei; Zhou, Ming

doi:10.1002/elps.201100693

Cited by 23 publications

(16 citation statements)

References 38 publications

(70 reference statements)

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“…Silver nitrate staining is also sensitive to a number of external factors such as the temperature and the development time making the Coomassie brilliant blue staining the preferred staining for proteomics. It is also possible to stain the proteins by using organic fluorescent dyes (such as Deep Purple TM , a fluorescent dye based upon the natural compound epicocconone, originally isolated from the fungus Epicoccum nigrum [ 30 ], Flamingo TM (Bio-Rad) and Krypton TM (Pierce) and metal complex or metal chelates dyes (such as SYPRO Red and Orange [ 31 ], the well-known being SYPRO Ruby [ 32 ], RuBPS [ 33 ], ASCQ_Ru [ 34 ]) and IrBPS [ 35 ]). This fluorescent staining is sensitive (ng to pg level), non-covalent (or reversible for epicocconone) and, consequently, compatible to MS.…”

Section: In-gel Quantification Of Proteinsmentioning

confidence: 99%

Comparative and Quantitative Global Proteomics Approaches: An Overview

et al. 2013

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Proteomics became a key tool for the study of biological systems. The comparison between two different physiological states allows unravelling the cellular and molecular mechanisms involved in a biological process. Proteomics can confirm the presence of proteins suggested by their mRNA content and provides a direct measure of the quantity present in a cell. Global and targeted proteomics strategies can be applied. Targeted proteomics strategies limit the number of features that will be monitored and then optimise the methods to obtain the highest sensitivity and throughput for a huge amount of samples. The advantage of global proteomics strategies is that no hypothesis is required, other than a measurable difference in one or more protein species between the samples. Global proteomics methods attempt to separate quantify and identify all the proteins from a given sample. This review highlights only the different techniques of separation and quantification of proteins and peptides, in view of a comparative and quantitative global proteomics analysis. The in-gel and off-gel quantification of proteins will be discussed as well as the corresponding mass spectrometry technology. The overview is focused on the widespread techniques while keeping in mind that each approach is modular and often recovers the other.

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Section: In-gel Quantification Of Proteinsmentioning

confidence: 99%

Comparative and Quantitative Global Proteomics Approaches: An Overview

et al. 2013

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“…3,4 The structure of RuBPS has inspired the successive development of BPS-based Ir(III) cyclometalated complexes with the general formula [Ir(C^N) 2 (BPS)], in which the peculiar and intense luminescent output of this class of metal complexes was combined with the "usual" protein recognition properties displayed by the sulfonate groups. 5 In these regards, it is worth noting that a recent report by Zhou and coworkers showed that also cationic and neutrally charged Ir(III) cyclometalated complexes devoid of any -SO 3 moieties could also be used as protein staining agents, pointing to the importance of other factors aside of electrostatic interactions in determining the performances of protein stains. 6 To the best of our knowledge, no examples of fac-triscarbonyl Re(I) diimines, one of the most extensively studied classes of luminescent d 6 metal complexes, have been considered for the specific purpose of protein staining.…”

Section: Introductionmentioning

confidence: 99%

Luminescent protein staining with Re(i) tetrazolato complexes

et al. 2018

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Within the general framework of our past and current studies dealing with the investigation of the photophysical properties and the biological behavior of the family of tetrazolato and tetrazole Re(i) complexes, we have endeavored to investigate their potential in the luminescent staining of proteins purified by acrylamide gel electrophoresis. With the aim to provide the first examples of luminescent Re(i) complexes to be exploited for this specific purpose, we have designed and prepared four new Re(i)-based species with the general formula fac-[Re(CO)3(N^N)(Tph)]2-/0, where Tph is the 5-(phenyl)tetrazolato anion and N^N is in turn represented by bathophenanthroline disulfonate (BPS), bathocuproine disulfonate (BCS) or by the SO3- free bathocuproine (BC). In this latter case, the neutral complex fac-[Re(CO)3(BC)(Tph)] served as a model species for the characterization of the former disulfonate complexes. Its cationic analogue fac-[Re(CO)3(BC)(Tph-Me)]+ was also prepared by a straightforward methylation reaction. All complexes displayed bright phosphorescence in organic media and, relative to their water solubility, the dianionic species fac-[Re(CO)3(BPS)(Tph)]2- and fac-[Re(CO)3(BCS)(Tph)]2- were also highly emissive in aqueous solution. The sulfonate groups played a key role in promoting and significantly enhancing the luminescent staining performances of both the Re(i) complexes fac-[Re(CO)3(BPS)(Tph)]2- and fac-[Re(CO)3(BCS)(Tph)]2- for proteins. Highlighting a response superior to that of Coomassie Blue and comparable to the one obtained by the well-known silver staining method, these dianionic Re(i)-complexes could efficiently detect up to 50 ng of pure Bovine Serum Albumin (BSA), as well as all proteins found in a Standard Protein Marker mix and from a total protein extract. A lower but still good response for luminescent protein staining was surprisingly obtained by employing the -SO3- free neutral and cationic complexes fac-[Re(CO)3(BC)(Tph)] and fac-[Re(CO)3(BC)(Tph-Me)]+, respectively. These preliminary results open up new possibilities for the further widening of the use of Re(i)-based complexes as luminescent protein staining agents.

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“…Following the publication of this work, 15,17 these and other bis-cyclometalated iridium complexes containing an ancillary BPS ligand ( Fig. 1a; 1-4) have been utilised for a variety of luminescence-based applications 5,12,[22][23][24] and they are now commercially available.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence detection with water-soluble iridium(iii) complexes containing a sulfonate-functionalised ancillary ligand

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Barbante

et al. 2014

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The chemiluminescence from four cyclometalated iridium(III) complexes containing an ancillary bathophenanthroline-disulfonate ligand exhibited a wide range of emission colours (green to red), and in some cases intensities that are far greater than the commonly employed benchmark reagent, [Ru(bpy)3](2+). A similar complex incorporating a sulfonated triazolylpyridine-based ligand enabled the emission to be shifted into the blue region of the spectrum, but the responses with this complex were relatively poor. DFT calculations of electronic structure and emission spectra support the experimental findings.

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Luminescent iridium(III) complexes as novel protein staining agents

Cited by 23 publications

References 38 publications

Comparative and Quantitative Global Proteomics Approaches: An Overview

Comparative and Quantitative Global Proteomics Approaches: An Overview

Luminescent protein staining with Re(i) tetrazolato complexes

Chemiluminescence detection with water-soluble iridium(iii) complexes containing a sulfonate-functionalised ancillary ligand

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