Luminal Leptin Enhances CD147/MCT-1-mediated Uptake of Butyrate in the Human Intestinal Cell Line Caco2-BBE

Buyse, Marion; Sitaraman, Shanthi V.; Liu, Xia; Bado, André; Merlin, Didier

doi:10.1074/jbc.m203281200

Cited by 108 publications

(85 citation statements)

References 47 publications

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“…Very little is also known about the regulation of MCT genes. Leptin has been shown to upregulate MCT1 mediated butyrate uptake in colonocytes (Buyse, Sitaraman, Liu, Bado, & Merlin, 2002) and erythropoietin increases the amount of MCT1 protein in erythrocyte membranes (Connes, Caillaud, Mercier, Bouix, & Casties, 2004).…”

Section: Monocarboxylate Transportersmentioning

confidence: 99%

Carbohydrate metabolism in meat animals

2005

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Oxidative energy production is by far dominant in living animal muscles, with the exception the short periods of severe stress, where the aerobic capacity is exceeded, and formation of large amounts of lactic acid will take place. Energy consumption in muscle cells continues post mortem with formation of large amounts of lactate and formation of protons, because the aerobic processes for energy production are not available. Post mortem, the fall in pH is delayed only by buffering capacity of the muscle fibres. In living animals, in addition to buffering capacity, both respiration and transport of lactate and protons out of the muscle fibres by monocarboxylate transporters participate in the regulation of muscle fibre pH which never falls as low as the ultimate pH of the meat. Understanding the regulation of pH in muscle is important both for the welfare of living animals and from the technological point of view as a factor influencing meat quality.

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Section: Monocarboxylate Transportersmentioning

confidence: 99%

Carbohydrate metabolism in meat animals

2005

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“…This was accomplished by studying the effect of Wnt11 on a well established culture model of intestinal epithelial differentiation, Caco2-BBE (45,52,53). These cells spontaneously undergo enterocyte differentiation upon confluency-mediated cell cycle arrest.…”

Section: Identification and Localization Of Wnt11 In Mousementioning

confidence: 99%

Wnt11 Signaling Promotes Proliferation, Transformation, and Migration of IEC6 Intestinal Epithelial Cells

Ouko

Ziegler

et al. 2004

Journal of Biological Chemistry

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“…The human enterocyte-like Caco2-BBE cell line [16][17][18][19] were grown in high glucose Dulbecco's Vogt-modified Eagle's media (DMEM, Invitrogen) supplemented with 14 mmol/l NaHCO 3 , 10% (v/v) heat-inactivated FBS and 1.5 mg/ml plasmocin. Cells were kept at 371C in 5% CO 2 and 90% humidity, and the medium was changed every other day.…”

Section: Cell Culturementioning

confidence: 99%

hPepT1 mediates bacterial tripeptide fMLP uptake in human monocytes

Charrier

Driss

Yan

et al. 2006

Laboratory Investigation

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Here, we examined hPepT1 expression in the monocytic cell line, KG-1. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that hPepT1 is expressed in KG-1 cells, while cDNA cloning and direct sequencing confirmed the sequence of KG-1 hPepT1 (accession number, AY634368). Immunoblotting of cell lysates from KG-1 cells or macrophages isolated from human peripheral blood revealed a B100 kDa immunoreactive band mainly present in the membrane fraction. Uptake experiments showed that the transport of 20 lM radiolabeled Gly-Sarcosine ([ 14 C]Gly-Sar) in KG-1 cells was Na þ , Cl À dependent and disodium 4,4 0 -diisothiocyanatostilbene-2,2 0 -disulfonate (DIDS)-sensitive. In addition, hPepT1 activity was likely to be coupled to a Na þ /H þ exchanger, as evidenced by the fact that [ 14 C]Gly-Sar uptake was not affected by the absence of Na þ when cells were incubated at low pH (5.2). Interestingly, hPepT1-mediated transport was reduced in KG-1 cells incubated at low pH as it was also observed in nonpolarized Caco2-BBE cells. This pattern of pH-dependence is due to a disruption of the driving force of hPepT1-mediated transport events. This was supported by our finding that nonpolarized cells, Caco2-BBE cells and KG-1 cells, have an increased permeability to H þ when compared to polarized Caco2-BBE cells. Finally, we showed that hPepT1 is responsible for transporting fMLP into undifferentiated and differentiated (macrophage-like) KG-1 cells. Together, these results show that hPepT1 is expressed in nonpolarized immune cells, such as macrophages, where the transporter functions best at the physiological pH 7.2. Furthermore, we provide evidence for hPepT1-mediated fMLP transport, which might constitute a novel immune cell activation pathway during intestinal inflammation.

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Luminal Leptin Enhances CD147/MCT-1-mediated Uptake of Butyrate in the Human Intestinal Cell Line Caco2-BBE

Cited by 108 publications

References 47 publications

Carbohydrate metabolism in meat animals

Carbohydrate metabolism in meat animals

Wnt11 Signaling Promotes Proliferation, Transformation, and Migration of IEC6 Intestinal Epithelial Cells

hPepT1 mediates bacterial tripeptide fMLP uptake in human monocytes

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